Investigation of combined effects of food factors for cancer prevention

• Project/Area Number: 19590593
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hygiene
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: SOWA Yoshihiro Kyoto Prefectural University of Medicine, 医学研究科, 准教授 (70315935)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 予防医学 / 食品成分 / diallyl trisulfide / reactive oxygen species / 併用 / 酪酸 / CDK / p21 / sulforaphane / dially trisulfide

Research Abstract

食品成分の併用による発癌予防効果の増強を検討する為に、まず単独での癌細胞増殖抑制を検討した。その結果、ニンニクに含まれるdiallyl trisulfideに増殖抑制効果があることを見いだした。次に増殖抑制成分が、単独では抑制効果を示さない濃度を設定し、同じく増殖抑成分である酪酸との併用効果を評価した。約30種類の各有効成分との併用を評価したが、充分な相加・相乗効果は見出せなかった。

Research Products

• [Journal Article] Insight into estrogenicity of phytoestrogens using in silico simulation (2009)
  • Author(s): Sugiyama H, Kumamoto T, Suganami A, Nakanishi W, Sowa Y, Takiguchi M, Ishikawa T, Tamura Y
  • Journal Title: Biochemical and Biophysical Research Communications 379(1) Pages: 139-14
  • Peer Reviewed
• [Journal Article] Insightinto estrogenicity of phytoestrogens using in silico simulation (2009)
  • Author(s): Sugiyama H., et al.
  • Journal Title: Biochemical and Biophysical Research Communications 379(1) Pages: 139-144
  • Peer Reviewed
• [Journal Article] Smenospongine, a sesquiterpene aminoquinone from a marine sponge, induces G1 arrest or apoptosis in different leulemia cells (2008)
  • Author(s): Kong D, Aoki S, Sowa Y, Sakai T, Kobayashi M
  • Journal Title: Marine Drugs 6(3) Pages: 480-488
  • Peer Reviewed
• [Journal Article] CDK inhibitor enhances the sensitivity to 5-fluorouracil in colorectal cancer cells. (2008)
  • Author(s): Takagi K, Sowa Y, Cevik OM, Nakanishi R, Sakai T
  • Journal Title: International Journal of Oncology 32(3) Pages: 1105-1111
  • Peer Reviewed
• [Journal Article] 発癌の分子機構に基づいた癌の"分子標的予防"研究 (2008)
  • Author(s): 曽和義広、酒井敏行
  • Journal Title: 医学のあゆみ 227(2) Pages: 147-148
• [Journal Article] Smenospongine, a sesquiterpene aminoquinone from a marine sponge, induces G1 arrest or apoptosis in different leulemia cells. (2008)
  • Author(s): Kong D., et al.
  • Journal Title: Marine Drugs 6(3) Pages: 480-488
  • Peer Reviewed
• [Journal Article] CDK inhibitor enhances the sensitivity to 5-fluorouracil in colorectal cancer cells. (2008)
  • Author(s): Takagi T., et al.
  • Journal Title: International Journal of Oncology 32(3) Pages: 1105-1110
  • Peer Reviewed
• [Journal Article] 発癌の分子機構に基づいた癌の“分子標的予防"研究 (2008)
  • Author(s): 曽和義広, 他
  • Journal Title: 医学のあゆみ 227(2) Pages: 147-148
• [Journal Article] Sulforaphane induces cell cycle arrest and apoptosis in murine osteosarcoma cells in vitro and inhibits tumor growth in vivo. (2007)
  • Author(s): Matsui TA, Murata H, Sakabe T, Sowa Y, Horie N, Nakanishi R, Sakai T, Kubo T
  • Journal Title: Oncology Reports 18(5) Pages: 1263-1268
  • Peer Reviewed
• [Journal Article] The plant alkaloid cryptolepine induces p21^<WAF1/CIP1> and cell cycle arrest in a human osteosarcoma cell line. (2007)
  • Author(s): Matsui TA, Sowa Y, Murata H, Takagi K, Nakanishi R, Aoki S, Yoshikawa M, Kobayashi M, Sakabe T, Kubo T, Sakai T
  • Journal Title: International Journal of Oncology 31(4) Pages: 915-922
  • Peer Reviewed
• [Journal Article] Sulforaphane induces cell cycle arrest and apoptosis in murine osteosarcoma cells in vitro and inhibits tumor growth in vivo. (2007)
  • Author(s): Matsui TA., et. al.
  • Journal Title: Oncology Reports 18(5) Pages: 1263-1268
  • Peer Reviewed
• [Journal Article] The plant alkaloid cryptolepine induces p21^<WAF1/CIP1> and cell cycle arrest in a human osteosarcoma cell line. (2007)
  • Author(s): Matsui TA., et. al.
  • Journal Title: International Journal of Oncology 31(4) Pages: 915-922
  • Peer Reviewed
• [Presentation] ニンニク成分diallyl trisulfideによるROSを介したヒト大腸癌細胞増殖抑制機構の解析 (2009)
  • Author(s): 曽和義広, 他
  • Organizer: 分子予防環境医学研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-01-23
• [Presentation] ニンニク成分diallyl trisulfide によるROSを介したヒト大腸癌細胞増殖抑制機構の解析 (2009)
  • Author(s): 曽和義広, 他
  • Organizer: 分子予防環境医学研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-01-23
• [Presentation] ニンニク成分diallyl trisulfideによるROSを介したヒト大腸癌細胞増殖抑制機構の解析 (2008)
  • Author(s): 曽和義広, 他
  • Organizer: 日本フードファクター学会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-17
• [Presentation] ニンニク成分diallyl trisulfide によるROSを介したヒト大腸癌細胞増殖抑制機構の解析 (2008)
  • Author(s): 曽和義広, 他
  • Organizer: 日本フードファクター学会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-17
• [Presentation] Identification of p15^<INK4b>-inductive compound, as a novel MEK 1/2 inhibitor (2008)
  • Author(s): Sowa Y., et.al.
  • Organizer: 日本癌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] Identification of p15^<INK4b> -inductive compound, as a novel MEK 1/2 inhibitor (2008)
  • Author(s): Sowa Y., et al.
  • Organizer: 日本癌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] CDK阻害因子p15^<INK4b> 誘導化合物の探索と新規MEK阻害剤としての同定 (2008)
  • Author(s): 曽和義広, 他
  • Organizer: がん分子標的治療研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-26
• [Presentation] CDK阻害剤による5-FU感受性増強効果に関する検討 (2008)
  • Author(s): 高木浩一, 他
  • Organizer: がん分子標的治療研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-26
• [Presentation] CDK阻害因子p15^<INK4b>誘導化合物の探索と新規MEK阻害剤としての同定 (2008)
  • Author(s): 曽和義広, 他
  • Organizer: がん分子標的治療研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-26
• [Presentation] ニンニク成分diallyl trisulfideによるROSを介した癌細胞増殖抑制機構の解析 (2008)
  • Author(s): 曽和義広, 他
  • Organizer: がん予防大会
  • Place of Presentation: 福岡
  • Year and Date: 2008-05-23
• [Presentation] ニンニク成分diallyl trisulfide によるROSを介しか癌細胞増殖抑制機構の解析 (2008)
  • Author(s): 曽和義広, 他
  • Organizer: がん予防大会
  • Place of Presentation: 福岡
  • Year and Date: 2008-05-23
• [Presentation] ニンニク成分diallyl trisulfideによるROSを介した癌細胞増殖抑制機構の解析 (2008)
  • Author(s): 曽和義広, 他
  • Organizer: 日本衛生学会
  • Place of Presentation: 熊本
  • Year and Date: 2008-03-30
• [Book] バイオ医薬の開発技術とシーズ (2009)
  • Author(s): 曽和義広
  • Publisher: シーエムシー出版
• [Book] バイオ医薬の開発技術とシース (2009)
  • Author(s): 曽和義広
  • Publisher: シーェムシー出版
• [Book] アンチエイジング・ヘルスフード (2008)
  • Author(s): 曽和義広, 他
  • Publisher: サイエンスフォーラム
• [Remarks]
  • URL: http://www2.kpu-m.ac.jp/~pubmed/
• [Remarks]
  • URL: http://www2.kpu-m.ac.jp/~pubmed/
• [Remarks]
  • URL: http://www2.kpu-m.ac.jp/~pubmed/