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The effect of exposure to chemical substance with bacterial toxin on the development of Th1/Th2 balance in infant mice.

Research Project

Project/Area Number 19590612
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hygiene
Research InstitutionNational Institute for Environmental Studies

Principal Investigator

YAMAMOTO Shoji  National Institute for Environmental Studies, 環境リスク研究センター, 主任研究員 (40230552)

Co-Investigator(Kenkyū-buntansha) FUJIMAKI Hidekazu  独立行政法人国立環境研究所, 環境リスク研究センター, 室長 (00124355)
HIYOSHI Kyoko  静岡県立大学, 看護学部, 講師 (90405580)
FUKUSHIMA Atsushi  独立行政法人国立環境研究所, 環境リスク研究センター, NIESポスドクフェロー (10442716)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords環境生理 / 小児健康影響 / アレルギー / 感染症 / 細菌 / 免疫学 / 有害化学物質
Research Abstract

The aims of the present study were to clarify whether the respiratory tract infection and stimulation can influence development of Th1 immune response and allergic reaction or not, and to investigate the effects of co-stimulation of the respiratory tract infectious agent with toluene which is one of the causative factors for chemical sensitivity and sick house syndrome can affect Th1/Th2 balance in infant mice using an allergic mouse model. Our findings indicate that (1) respiratory tract infection and stimulation with cell wall component of gram positive bacteria, peptidoglycan (PGN) does not affect development of Th1 immune response and allergic reaction in infant mice with ovalbumin (OVA) immunization ; (2) subcutaneous inoculation with BCG vaccine enhanced Th1 response via increased plasma IgG2a antibody and IFN-γexpression in lung and spleen of OVA immunized mice (this finding clearly shows that although BCG inoculation did not induce respiratory tract stimulation, it can be used for induction of Th1 response) ; (3) low level toluene exposure only from fetal to neonatal stage may enhance the systemic Th2 function in infant mice, however, the co-exposure of low level toluene with PGN shifted Th1/Th2 immunity to suppressive direction ; (4) in addition, the co-exposure of low level toluene with PGN in infant stage altered both Th1 and Th2 dependent blood Ig antibody level in 3 week-old mice.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (38 results)

All 2010 2009 2008 2007 Other

All Journal Article (19 results) (of which Peer Reviewed: 3 results) Presentation (16 results) Remarks (3 results)

  • [Journal Article] Up-regulation of neurotrophin-related gene expression in mouse hippocampus following low-level toluene exposure2010

    • Author(s)
      in-Shwe, T.T., Tsukahara, S., Yamamoto, S., Fukushima, A., Kunugita, N., Arashidani, K., Fujimaki, H.
    • Journal Title

      Neuro. Toxicol. 31(1)

      Pages: 85-93

    • Related Report
      2009 Final Research Report
  • [Journal Article] 揮発性有機化合物の胎仔期曝露による仔マウスのTh1, Th2型免疫応答のかく乱2010

    • Author(s)
      山元昭二・Tin-Tin-Win-Shwe・藤巻秀和
    • Journal Title

      岡山実験動物研究会報誌 26

      Pages: 45-49

    • Related Report
      2009 Final Research Report
  • [Journal Article] 揮発性有機化合物の胎仔期曝露による仔マウスのTh1, Th2型免疫応答のかく乱2010

    • Author(s)
      山元昭二・Tin-Tin Win-Shwe・藤巻秀和
    • Journal Title

      岡山実験動物研究会報誌 26

      Pages: 45-49

    • Related Report
      2009 Annual Research Report
  • [Journal Article] The expression of nerve growth factor in mice lung following low-level toluene exposure2009

    • Author(s)
      Fujimaki, H., Win-Shwe, T.T., Yamamoto, S., Nakajima, D., Goto, S.
    • Journal Title

      Toxicol. Lett. 191

      Pages: 240-245

    • Related Report
      2009 Final Research Report
  • [Journal Article] Suppression of Th1-and Th2-type immune responses in infant mouse spleen after prenatal and postnatal exposure to low-level toluene and peptidoglycan2009

    • Author(s)
      Yamamoto, S., Win-Shwe, T.T., Yoshida, Y., Kunugita, N., Arashidani, K., Fujimaki, H.
    • Journal Title

      Inhal. Toxicol. 21(9)

      Pages: 793-802

    • Related Report
      2009 Final Research Report
  • [Journal Article] Children's immunology, what can we learn from animal studies (2): Modulation of systemic Th1/Th2 immune response in infant mice after prenatal exposure to low-level toluene and toll-like receptor (TLR) 2 ligand2009

    • Author(s)
      Yamamoto, S., Win-Shwe, T.T., Yoshida, Y., Kunugita, N., Arashidani, K., Fujimaki, H.
    • Journal Title

      J. Toxicol. Sci. 34

    • Related Report
      2009 Final Research Report
  • [Journal Article] Modulation of systemic Th1/Th2 immune response in infant mice after prenatal exposure to low-level toluene and toll-like receptor(TLR)2 ligand2009

    • Author(s)
      Yamamoto, S., Win-Shwe, T.T., Yoshida, Y., Kunugita, N., Arashidani, K., Fujimaki, H.
    • Journal Title

      J.Toxicol.Sci. 34

      Pages: 341-348

    • NAID

      110007337702

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Suppression of Th1- and Th2- type immune responses in infant mouse spleen after prenatal and postnatal exposure to low-level toluene and peptidoglycan2009

    • Author(s)
      Yamamoto, S., Win-Shwe, T.T., Yoshida, Y., Kunugita, N., Arashidani, K., Fujimaki, H.
    • Journal Title

      Inhal.Toxicol. 21

      Pages: 793-802

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 時間的感受性要因に注目した化学物質の健康影響評価-マウス胎児期・授乳期における免疫・感染指標の検討-2008

    • Author(s)
      山元昭二
    • Journal Title

      国立環境研究所特別研究報告書「環境リスク研究プログラム(中間報告)

      Pages: 18-19

    • Related Report
      2009 Final Research Report
  • [Journal Article] Establishment of a Mouse Model to Asses Brain Neurotransmitter Level and Leaning Performance Simultaneously Following Toxic Chemical Exposure: Using in vivo Microdialysis and Schedule-Controlled Operant Behavior2008

    • Author(s)
      Win-Shwe, T.T., Hojo, R., Mitsushima, D., Nakajima, D., Yamamoto, S., Fujimaki, H.
    • Journal Title

      J. UOEH. 31(1)

      Pages: 1-11

    • Related Report
      2009 Final Research Report
  • [Journal Article] Strain Differences in Extracellular Amino Acid Neurotransmitter Levels in the Hippocampus of Major Histocompatibility Complex Congenic Mice in Response to Toluene Exposure2008

    • Author(s)
      Win-Shwe, T.T., Mitsushima, D., Yamamoto, S., Funabashi, T., Fujimaki, H.
    • Journal Title

      Neuroimmunomodulation 16(3)

      Pages: 185-190

    • Related Report
      2009 Final Research Report
  • [Journal Article] Role of CD4 (+) T cells in the modulation of neurotrophin production in mice exposed to low-level toluene2008

    • Author(s)
      Fujimaki, H., Win-Shwe, T.T., Yamamoto, S., Nakajima, D., Goto, S.
    • Journal Title

      Immunopharmacol. Immunotoxicol. 31(1)

      Pages: 146-149

    • Related Report
      2009 Final Research Report
  • [Journal Article] Role of CD4(+) T cells in the modulation of neurotrophin production in mice exposed to low-level toluene2008

    • Author(s)
      Fujimaki, H., Win Shwe, T. T., Yamamoto, S., Nakajima, D., Goto, S.
    • Journal Title

      Immunopharmacol. Immunotoxicol. 31

      Pages: 146-149

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Toluene induces rapid and reverse of hippocampal glutamate and taurine neurotransmitter levels in mice2007

    • Author(s)
      Win-Shwe, T.T., Mitsushima, D., Nakajima, D., Ahmed, S., Yamamoto, S., Tsukahara, S., Kakeyama, M., Goto, S., Fujimaki, H.
    • Journal Title

      Toxicol. Lett. 168

      Pages: 76-82

    • Related Report
      2009 Final Research Report
  • [Journal Article] Effect of long-term exposure to low-level toluene on airway inflammatory response in mice2007

    • Author(s)
      Fujimaki, H., Yamamoto, S., Win-Shwe, T.T., Hojo, R., Sato, F., Kunugita, N., Arashidani, K.
    • Journal Title

      Toxicol. Lett. 168

      Pages: 132-139

    • Related Report
      2009 Final Research Report
  • [Journal Article] Effects of low-level formaldehyde exposure on synaptic plasticity related gene expression in the hippocampus of immunized mice2007

    • Author(s)
      Ahmed, S., Tsukahara, S., Win-Shwe, T.T., Yamamoto, S., Kunugita, N., Arashidani, K., Fujimaki, H.
    • Journal Title

      J. Neuroimmunol. 186

      Pages: 104-111

    • Related Report
      2009 Final Research Report
  • [Journal Article] Modulation of neurological related allergic reaction in mice exposed to low-level toluene2007

    • Author(s)
      Win Shwe, T.T., Yamamoto, S., Nakajima, D., Furuyama, A., Fukushima, A., Ahmed, S., Goto, S., Fujimaki, H.
    • Journal Title

      Toxicol. Appl. Pharmacol. 222

      Pages: 17-24

    • Related Report
      2009 Final Research Report
  • [Journal Article] Athymic nude mice are insensitive to low-level toluene-induced up-regulation of memory-related gene expressions in the hippocampus2007

    • Author(s)
      Win-Shwe, T.T., Tsukahara, S., Ahmed, S., Fukushima, A., Yamamoto, S., Kakeyama, M., Nakajima, D., Goto, S., Kobayashi, T., Fujimaki, H.
    • Journal Title

      Neurotoxicology 28

      Pages: 957-964

    • Related Report
      2009 Final Research Report
  • [Journal Article] Increased hippocampal mRNA expression of neuronal synaptic plasticity related genes in mice chronically exposed to toluene at a low-level human occupational-exposure2007

    • Author(s)
      Ahmed, S., Win-Shwe, T.T., Yamamoto, S., Tsukahara, S., Kunugita, N., Arashidani, K., Fujimaki, H.
    • Journal Title

      Neurotoxicology 28

      Pages: 168-174

    • Related Report
      2009 Final Research Report
  • [Presentation] マウス胎仔期・新生仔期・乳仔期における低濃度トルエン曝露が免疫系の発達に及ぼす影響2009

    • Author(s)
      山元昭二, Tin-Tin-Win-Shwe, 欅田尚樹, 吉田安宏, 嵐谷奎一, 藤巻秀和
    • Organizer
      第16回日本免疫毒性学会学術大会
    • Place of Presentation
      旭川市民文化会館(北海道)
    • Year and Date
      2009-08-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] A potential role of hippocampal memory function-related gene expression in developmental neurotoxicity of toluene2009

    • Author(s)
      Win-Shwe, T.T., Kunugita, N., Yamamoto, S., Arashidani, K., Fujimaki, H.
    • Organizer
      13th Congr. Eur. Fed. Neurol. Soc.
    • Place of Presentation
      Florence
    • Related Report
      2009 Final Research Report
  • [Presentation] Strain differences influence synaptic plasticity-related gene expression in olfactory bulb of a mouse model of allergy following toluene exposure2009

    • Author(s)
      Fujimaki, H., Win-Shwe T.T., Kunugita, N., Yamamoto, S., Arashidani, K.
    • Organizer
      13th Congr. Eur. Fed. Neurol. Soc.
    • Place of Presentation
      Florence
    • Related Report
      2009 Final Research Report
  • [Presentation] マウス胎仔期・新生仔期・乳仔期における低濃度トルエン曝露が免疫系の発達に及ぼす影響2009

    • Author(s)
      山元昭二・Tin-Tin-Win-Shwe・欅田尚樹・吉田安宏・嵐谷奎一・藤巻秀和
    • Organizer
      第16回日本免疫毒性学会学術大会
    • Place of Presentation
      旭川
    • Related Report
      2009 Final Research Report
  • [Presentation] Effect of perinatal toluene exposure at different developmental phases on memory function-related gene expressions in mouse brain2009

    • Author(s)
      Tin-Tin-Win-Shwe, Kunugita, N., Yamamoto, S., Arashidani, K., Fujimaki, H.
    • Organizer
      50th Annu. Meet. Jpn. Soc. Atomos. Environ.
    • Place of Presentation
      Yokohama
    • Related Report
      2009 Final Research Report
  • [Presentation] マウス系統差で探るトルエン曝露に対する免疫系感受性要因2009

    • Author(s)
      欅田尚樹・Tin-Tin-Win-Shwe・山元昭二・吉田安宏・嵐谷奎一・藤巻秀和
    • Organizer
      第50回大気環境学会年会
    • Place of Presentation
      横浜
    • Related Report
      2009 Final Research Report
  • [Presentation] Effects of toluene exposure on developmental immunotoxicity in infant mice brain2009

    • Author(s)
      Tin-Tin-Win-Shwe, Kunugita, N., Yamamoto, S., Arashidani, K., Fujimaki, H.
    • Organizer
      16th Annu. Meet. Jpn. Soc. Immunotoxicol.
    • Place of Presentation
      Asahikawa
    • Related Report
      2009 Final Research Report
  • [Presentation] アスベスト肺に対する常在細菌による急性曝露の影響2009

    • Author(s)
      日吉孝子・山元昭二・井上健一郎・市瀬孝道
    • Organizer
      第16回日本免疫毒性学会学術大会
    • Place of Presentation
      旭川
    • Related Report
      2009 Final Research Report
  • [Presentation] トルエンの発達期影響-感染免疫-2009

    • Author(s)
      山元昭二
    • Organizer
      第24回全国環境研究所交流シンポジウム「揮発性有機化合物(VOC)の動態と健康影響」
    • Place of Presentation
      つくば
    • Related Report
      2009 Final Research Report
  • [Presentation] VOCと免疫毒性2009

    • Author(s)
      欅田尚樹・吉田安宏・嵐谷奎一・Tin-Tin-Win-Shwe・山元昭二・藤巻秀和
    • Organizer
      第24回全国環境研究所交流シンポジウム「揮発性有機化合物(VOC)の動態と健康影響」
    • Place of Presentation
      つくば
    • Related Report
      2009 Final Research Report
  • [Presentation] Modulation of T cell development by biological and chemical agents neonatal mice2008

    • Author(s)
      Fujimaki, H., Win-Shwe, T.T., Yamamoto. S.
    • Organizer
      7th Annu. Eur. Life Sci. Org. Meet.
    • Place of Presentation
      Nice
    • Related Report
      2009 Final Research Report
  • [Presentation] トルエン曝露によるMHC-congenicマウス海馬における神経伝達物質レベルの変化2008

    • Author(s)
      Tin-Tin-Win-Shwe・美津島大・山元昭二・藤巻秀和
    • Organizer
      第15回日本免疫毒性学会学術大会
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] マウス乳児期におけるトルエン曝露とBCG刺激がTh1/Th2バランスの発達に及ぼす影響2008

    • Author(s)
      山元昭二・Tin-Tin Win-Shwe・藤巻秀和
    • Organizer
      第15回日本免疫毒性学会学術大会
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] 低濃度トルエン曝露が発達期のマウス免疫系に及ぼす影響2008

    • Author(s)
      山元昭二・藤巻秀和
    • Organizer
      第35回日本トキシコロジー学会学術年会-こどもシンポジウム5-
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] マウス胎生期および新生児期における低濃度トルエン曝露とペプチドグリカン刺激が全身性のTh1/Th2免疫バランスに及ぼす影響2007

    • Author(s)
      山元昭二・福島篤・Tin-Tin-Win-Shwe・北條理恵子・藤巻秀和
    • Organizer
      第14回日本免疫毒性学会学術大会
    • Place of Presentation
      神戸
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Presentation] トルエン曝露によるマウス海馬における記憶関連遺伝子発現増強へのT細胞の役割2007

    • Author(s)
      Tin-Tin-Win-Shwe・山元昭二・藤巻秀和
    • Organizer
      第14回日本免疫毒性学会学術大会
    • Place of Presentation
      神戸
    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.nies.go.jp/risk/members/yamamoto/yamamotoj.htmlhttp://www.nies.go.jp/risk/mei/mei003_4.html

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.nies.go.jp/risk/mei/mei003_4.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.nies.go.jp/risk/members/yamamoto/yamamotoj.html

    • Related Report
      2009 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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