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Promotion of rapid and high-throughput SNP typing for human identification in Forensic practices

Research Project

Project/Area Number 19590667
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionTohoku University

Principal Investigator

HASHIYADA Masaki  Tohoku University, 大学院・医学系研究科, 技術専門員 (40374938)

Co-Investigator(Kenkyū-buntansha) SAKAI Jun  東北大学, 大学院医学系研究科, 助教 (20431504)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
KeywordsDNA多型医学 / SNP / 社会医学 / 核酸 / ゲノム / 個人 / 暗号・認証等 / 個人識別
Research Abstract

次のsingle nucleotide polymorphism(SNP)データベースを構築し, その個人識別有用性を確認した. 常染色体上SNP150座位(100名),Y染色体上SNP24座位(243名), およびミトコンドリアDNA上のSNP24座位(215名)である. さらに, 通常は約45分かかるFast TaqMan法の反応時間を検討し,DNAの抽出からタイピング結果を得るまで,わずか30分しか要さない反応系を確立した. また, Microsoft Excelによるスプレッドシートを新たに作製することで, 一度に最大96SNPの解析を可能にし, 識別精度の向上が図られた.

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (26 results)

All 2009 2008 2007

All Journal Article (7 results) (of which Peer Reviewed: 6 results) Presentation (13 results) Book (6 results)

  • [Journal Article] Polymorphism of eight X-chromosomal STRs in a Japanese population2008

    • Author(s)
      Masaki Hashiyada, Yukio Itakura, Masato Funayama
    • Journal Title

      Forensic Sci. Int. Genetics Supplement Series 1

      Pages: 150-152

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Population genetics of 17 Y-chromosomal STR loci in Japanese2008

    • Author(s)
      Masaki Hashiyada, Kazuo Umetsu, Isao Yuasa, Akiyoshi Tamura, Aya Matsusue, Koichi Suzuki, Seiichi Kashimura, and Masato Funayama
    • Journal Title

      Forensic Sci. Int. Genetics Vol.2

      Pages: 69-70

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Population genetics of 17 Y-chromosomal STR loci in Japanese2008

    • Author(s)
      Masaki Hashiyada, et.al.
    • Journal Title

      Forensic Science International Genetics 2

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Polymorphism of eight X-chromosomal STRs in a Japanese population2008

    • Author(s)
      Masaki Hashiyada, et.al.
    • Journal Title

      Forensic Science International Genetics Supplement Series 1

      Pages: 150-152

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Paradox in the Biometric Personal Authentication System Using STR Polymorphism2007

    • Author(s)
      Hashiyada M, Itakura Y, Funayama M.
    • Journal Title

      - Practical Matching Probabilities Evaluate the DNA personal ID System -. Res Pract Forens Med 50

      Pages: 235-239

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Birthday Paradox in the Biometric Personal Authentication System using STR Polymorphism -Personal Matching Probabilities Evaluate the DNA Personal ID System-2007

    • Author(s)
      Masaki Hashiyada, Jun Sakai, Toshio Nagashima, et. al.
    • Journal Title

      Res Pract Forens Med 50

      Pages: 235-239

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SNPデータベースの構築と解析の高速化2007

    • Author(s)
      橋谷田真樹, 板倉征男, 長嶋登志男他
    • Journal Title

      DNA多型 15

      Pages: 293-295

    • Related Report
      2007 Annual Research Report
  • [Presentation] 新たな一塩基多型(SNP)情報による個人識別精度の向上2009

    • Author(s)
      橋谷田真樹, 板倉征男, 舟山眞人
    • Organizer
      2009年暗号と情報セキュリティシンポジウム
    • Place of Presentation
      大津.大津プリンスホテル
    • Related Report
      2008 Final Research Report
  • [Presentation] 新たな-塩基多型(SNP)情報による個人識別精度の向上2009

    • Author(s)
      橋谷田真樹, 板倉征男, 舟山眞人
    • Organizer
      2009年暗号と情報セキュリティシンポジウム
    • Place of Presentation
      大津プリンスホテル, 大津
    • Related Report
      2008 Annual Research Report
  • [Presentation] 日本人におけるSNPsデータベースの充実と識別精度向上の検討2008

    • Author(s)
      橋谷田真樹, 板倉征男, 舟山眞人
    • Organizer
      日本DNA多型学会第17回学術集会
    • Place of Presentation
      東京.私学会館
    • Related Report
      2008 Final Research Report
  • [Presentation] Haplogroup determination of mitochondrial DNA in Japanese using single nucleotide polymorphisms analysis2008

    • Author(s)
      Masaki Hashiyada, Yukio Itakura, Masato Funayama
    • Organizer
      The 19th International Symposium on Human Identification
    • Place of Presentation
      Identification. Hollywood, California, USA.
    • Related Report
      2008 Final Research Report
  • [Presentation] Development of a spreadsheet for SNPs typing using MicrosoftR EXCEL2008

    • Author(s)
      Masaki Hashiyada, Yukio Itakura, Shirushi Takahashi, Jun Sakai, Masato Funayama
    • Organizer
      The 7th International Symposium Advances in Legal Medicine
    • Place of Presentation
      Osaka, JAPAN
    • Related Report
      2008 Final Research Report
  • [Presentation] Development of a spreadsheet for SNPs typing using Microsoft^R EXCEL2008

    • Author(s)
      Masaki Hashiyada, Yukio Itakura, Masato Funayama, et.al.
    • Organizer
      The 7th International Symposium Advances in Legal Medicine
    • Place of Presentation
      Osaka, Japan
    • Related Report
      2008 Annual Research Report
  • [Presentation] Haplogroup determination of mitochondrial DNA in Japanese using single nucleotide polymorphisms analysis2008

    • Author(s)
      Masaki Hashiyada, Yukio Itakura, Masato Funayama
    • Organizer
      The 19th International Symposium on Human Identification
    • Place of Presentation
      Hollywood, California, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] 日本人におけるSNPsデータベースの充実と識別精度向上の検討2008

    • Author(s)
      橋谷田真樹, 板倉征男, 舟山眞人
    • Organizer
      日本DNA多型学会第17回学術集会
    • Place of Presentation
      私学会館, 東京
    • Related Report
      2008 Annual Research Report
  • [Presentation] Fast TaqMan法を用いた日本人におけるY-SNP解析2007

    • Author(s)
      橋谷田真樹, 板倉征男, 舟山眞人
    • Organizer
      日本DNA多型学会第16回学術集会
    • Place of Presentation
      大阪
    • Related Report
      2008 Final Research Report
  • [Presentation] Population genetics of eight X-chromosomal STRs in a Japanese population2007

    • Author(s)
      Hashiyada M, Itakura Y, Funayama M.
    • Organizer
      The 18th International Symposium on Human Identification
    • Place of Presentation
      Hollywood, California, USA.
    • Related Report
      2008 Final Research Report
  • [Presentation] Polymorphism of eight X-chromosomal STRs in a Japanese population2007

    • Author(s)
      Hashiyada M, Itakura Y, Funayama M.
    • Organizer
      22nd Congress of the International Society for Forensic Genetics
    • Place of Presentation
      Copenhagen, Denmark
    • Related Report
      2008 Final Research Report
  • [Presentation] Studies on differentially methylated parental allele in imprinted genes2007

    • Author(s)
      Nakayashiki N, Takamiya M, Shimamoto K, Aoki Y, Hashiyada M.
    • Organizer
      22nd Congress of the International Society for Forensic Genetics
    • Place of Presentation
      Copenhagen, Denmark
    • Related Report
      2008 Final Research Report
  • [Presentation] 複数の人間をSNPで検出するには何座位必要か?2007

    • Author(s)
      境純, 橋谷田真樹, 高橋識志, 金涌佳雅, 金武潤, 舟山眞人
    • Organizer
      第91次日本法医学会総会
    • Place of Presentation
      秋田
    • Related Report
      2008 Final Research Report
  • [Book] Fast TaqMan法を用いた日本人におけるY-SNP解析. DNA多型162008

    • Author(s)
      橋谷田真樹, 板倉征男, 舟山眞人
    • Publisher
      東京 : 東洋書籍
    • Related Report
      2008 Final Research Report
  • [Book] DNA多型Vol.162008

    • Author(s)
      橋谷田真樹, 板倉征男, 舟山眞人
    • Publisher
      Fast TaqMan法を用いた日本人におけるY-SNP解析
    • Related Report
      2008 Annual Research Report
  • [Book] SNPデータベースの構築と解析の高速化. DNA多型152007

    • Author(s)
      橋谷田真樹、板倉征男、長嶋登志男、境純、舟山眞人
    • Publisher
      東京 : 東洋書籍
    • Related Report
      2008 Final Research Report
  • [Book] 擬父と擬父の祖父のいずれかが生物学的な父親かを問う場合の父権肯定確率計算法. DNA多型15.2007

    • Author(s)
      境純、橋谷田真樹、金武 潤、舟山眞人
    • Publisher
      東京 : 東洋書籍
    • Related Report
      2008 Final Research Report
  • [Book] DMPA(differentiallymethylated parental allele)検出法に関する検討III.DNA多型15.2007

    • Author(s)
      中屋敷徳, 高宮正隆, 熊谷章子, 青木康博, 橋谷田真樹
    • Publisher
      東京 : 東洋書籍
    • Related Report
      2008 Final Research Report
  • [Book] 2007年 暗号と情報セキュリティシンポジウム(SCIS2007)予稿集2007

    • Author(s)
      橋谷田真樹, 斉藤 直, 板倉征男, 舟山眞人
    • Publisher
      一塩基多型(SNP)による個人識別の実験的検証と解析の高速化(CD-ROM)
    • Related Report
      2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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