Development of solid-phase e traction tips for analysis of drugs and poisons and its forensic application

• Project/Area Number: 19590679
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Showa University
• Principal Investigator: KUMAZAWA Takeshi Showa University, 医学部, 教授 (00186470)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 固相抽出 / 固相抽出チップ / 薬毒物分析 / GC法 / GC / MS法

Research Abstract

人体試料中覚せい剤(メタンフェタミン、アンフェタミン)、麻薬(コデイン、ジヒドロコデイン)、向精神薬(三環系、フェノチアジン系、ブチロフェノン系、ベンゾジアゼピン系)及び農薬(有機リン系、カーバメート系)について、固相抽出チップを用いた抽出法とガスクロマトグラフィー/質量分析計による分離・検出法を組み合わせた新しい薬毒物分析法の開発を試みた。その結果、抽出に使用する人体試料と溶媒の微量化が達成され、分析対象物質の回収率及び分離性が良く、定量性にも優れた、簡便で迅速性のある分析システムを構築することができた。また、本システムは実際に薬物を服用した人体試料あるいは法医実際例において、その有用性を確認することができた。

Research Products

• [Journal Article] Automated on-line in-tube solid-phase microextraction coupled with HPLC/MS/MS for the determination of butyrophenone derivatives in human plasma (2009)
  • Author(s): Kumazawa T., Saeki, K., Yanagisawa I., Uchigasaki S., Hasegawa C., Seno H., Suzuki O., Sato K.
  • Journal Title: Anal. Biochem. Chem. (印刷中)
  • Peer Reviewed
• [Journal Article] High-throughput determination of theophylline and caffeine in human serum by conventional liquid chromatography-mass spectrometry (2009)
  • Author(s): Arinobu T., Hattori H., Kumazawa T., Lee X.-P., Mizutani Y., Katase T., Kojima S., Omori T., Kaneko R., Ishii A., Seno H.
  • Journal Title: Forensic Toxicol. 27 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Simultaneous determination of some phenothiazine derivatives in human blood by headspace solid-phase microextraction and gas chromatography with nitrogen- phosphorus detection (2008)
  • Author(s): Shinmen N., Lee X.-P., Kumazawa T., Hasegawa C., Ishiwata Y., Sato K., Seno H., Suzuki O.
  • Journal Title: J. AOAC Int. 91 Pages: 1354-1362
  • Peer Reviewed
• [Journal Article] Utility of disk solid-phase extraction for whole blood samples: analysis of some tetracyclic antidepressants by gas chromatography with nitrogen- phosphorus detection (2008)
  • Author(s): Marumo A., Kumazawa T., Lee X.-P., Hasegawa C., Suzuki O., Sato K.
  • Journal Title: Forensic Toxicol. 26 Pages: 16-18
  • Peer Reviewed
• [Journal Article] Determination of diazepam and its metabolites in human urine by liquid chromatography/tandem mass spectro- metry using a hydrophilic polymer column. Rapid Commun. (2008)
  • Author(s): Umezawa H., Lee X.-P., Arima Y., Hasegawa C., Marumo A., Kumazawa T., Sato K.
  • Journal Title: Mass Spectrom. 22 Pages: 2333-2341
  • Peer Reviewed
• [Journal Article] Determination of tricyclic anti- depressants in human plasma using pipette tip solid-phase extraction and gas chromatography-mass spectrometry (2008)
  • Author(s): Lee X.-P., Hasegawa C., Kumazawa T., Shinmen N., Shoji U., Seno H., Sato K.
  • Journal Title: J. Sep. Sci. 31 Pages: 2265-2271
  • Peer Reviewed
• [Journal Article] Determination of tricyclic antidepressants in human plasma using pipette tipsolid-phase extraction and gas chromatography-mass spectrometry. (2008)
  • Author(s): Xiao-Pen Lee
  • Journal Title: J. Sep. Sci. 31 Pages: 2265-2271
  • Peer Reviewed
• [Journal Article] Simultaneous determination of some phenothiazine derivatives in human blood by headspace solid-phase microextraction and gas chromatography with nitrogen-phosphorus detection. (2008)
  • Author(s): Natsuko Shinmen
  • Journal Title: J. AOAC Int. 91 Pages: 1354-1362
  • Peer Reviewed
• [Journal Article] Pipette tip solid-phase extraction and gas chromatography?mass spectrometry for the determination of methamphetamine and amphetamine in human whole blood (2007)
  • Author(s): Hasegawa C., Kumazawa T., Lee X.-P., Shinmen N., Marumo A., Seno H., Sato K.
  • Journal Title: Anal. Bioanal. Chem. 389 Pages: 563-570
  • Peer Reviewed
• [Journal Article] An automated on-line method for simultaneous analysis of phenothiazines in human serum by high-performance liquid chromatography/ sonic spray ionization mass spectrometry using backflash column switching (2007)
  • Author(s): Aoki R., Arinobu T., Kumazawa T., Hattori H., Noguchi H.
  • Journal Title: Forensic Toxicol. 25 Pages: 8-15
  • Peer Reviewed
• [Journal Article] Simultaneous determination of methamphetamine and amphetamine in human urine using pipette tip solid-phase extraction and gas chromatography-mass spectrometry (2007)
  • Author(s): Kumazawa T., Hasegawa C., Lee X.-P., Hara K, Seno H., Suzuki O., Sato K.
  • Journal Title: J. Pharm. Biomed. Anal. 44 Pages: 602-607
  • Peer Reviewed
• [Journal Article] Simultaneous determination of methamphetamine and amphetamine in human urineusing pipette tip solid-phase extraction and gas chromatography-masss pectrometry (2007)
  • Author(s): Takeshi Kumazawa
  • Journal Title: J.Pharm.Biomed.Anal. 44・2 Pages: 602-607
  • Peer Reviewed
• [Journal Article] Pipette tip solid-phase extraction and gas chromatography-mass spectrometry forthe determination of methamphetamine and amphetamine in human whole blood (2007)
  • Author(s): Chika Hasegawa
  • Journal Title: Anal.Bioanal.Chem. 389・2 Pages: 563-570
  • Peer Reviewed
• [Presentation] 生体試料中薬毒物のGC/MS分析における固相抽出チップの開発と応用 (2008)
  • Author(s): 長谷川智華, 熊澤武志, 佐藤啓造
  • Organizer: 第33回日本医用マススペクトル学会年会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-26
• [Presentation] ダイレクト注入HPLC-MS/MS法の生体試料中薬物及び代謝物分析への応用 (2008)
  • Author(s): 李暁鵬, 熊澤武志, 佐藤啓造
  • Organizer: 第33回日本医用マススペクトル学会年会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-26
• [Presentation] 生体試料中薬毒物のGC/MS分析における固相抽出チップの開発と応用 (2008)
  • Author(s): 長谷川 智華
  • Organizer: 第33回日本医用マススペクトル学会年会
  • Place of Presentation: 東京大学医学部鉄門記念講堂
  • Year and Date: 2008-09-26
• [Presentation] 新規固相抽出技術の法中毒学的応用に関する研究 (2008)
  • Author(s): 熊澤 武志
  • Organizer: 日本法中毒学会第27年会
  • Place of Presentation: 昭和大学上條講堂
  • Year and Date: 2008-06-13
• [Presentation] 新規固相抽出技術の法中毒学的応用に関する研究 (2008)
  • Author(s): 熊澤 武志
  • Organizer: 日本法中毒学会第27年会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-01
• [Presentation] HPLC-MS/MSによる尿中ジアゼパム及び関連代謝物の簡易分析法 (2008)
  • Author(s): 梅澤宏亘, 李暁鵬, 有馬由子, 長谷川智華, 丸茂明美, 熊澤武志, 佐藤啓造
  • Organizer: 第92次日本法医学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2008-04-02
• [Presentation] 直接注入HPLC-MS/MS法による尿中ジアゼパムおよび代謝物の一斉分析 (2007)
  • Author(s): 梅澤宏亘, 有馬由子, 李暁鵬, 熊澤武志, 佐藤啓造
  • Organizer: 第76回日本法医学会関東地方会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-20
• [Presentation] オンライン/オフライン試料前処理法とLC/MS/MS法による生体試料中の薬毒物分析 (2007)
  • Author(s): 熊澤武志, 李暁鵬, 佐藤啓造, 鈴木修
  • Organizer: 第32回日本医用マススペクトル学会年会
  • Place of Presentation: 京都
  • Year and Date: 2007-09-28
• [Presentation] 固相抽出チップを用いた尿中覚せい剤の分析 (2007)
  • Author(s): 熊澤武志, 長谷川智華, 李暁鵬, 宮崎将太, 原健二, 妹尾洋, 鈴木修, 佐藤啓造
  • Organizer: 日本法中毒学会第26年会
  • Place of Presentation: 宮崎
  • Year and Date: 2007-06-08
• [Presentation] 固相抽出チップを用いた尿中覚せい剤の分析 (2007)
  • Author(s): 熊澤 武志
  • Organizer: 日本法中毒学会第26年会
  • Place of Presentation: 九州保健福祉大学(宮崎)
  • Year and Date: 2007-06-08
• [Presentation] 直接注入HPLC/MS/MSによるヒト血漿中β遮断薬の簡易分析法 (2007)
  • Author(s): 梅澤宏亘, 李暁鵬, 熊澤武志, 有馬由子, 丸茂明美, 長谷川智華, 新免奈津子, 妹尾洋, 佐藤啓造
  • Organizer: 第91次日本法医学会総会
  • Place of Presentation: 秋田
  • Year and Date: 2007-05-18
• [Presentation] Determination of phenothiazines in human serum by HPLC/SSI-MS using backflush column-switching (2007)
  • Author(s): Arinobu T., Aoki R., Kumazawa T., Lee X.-P., Ishii A., Kaneko R., Kojima S., Noguchi H., Suzuki O., Hattori H., Seno H.
  • Organizer: 12th International meeting on recent developments in pharmaceutical analysis
  • Place of Presentation: Elba, Italy
• [Presentation] Analysis of psychotropic drugs in human body fluids using disk solid-phase extraction. T-2007-A Joint Meeting of The International Association of Forensic Toxicologists (TIAFT) and the International Council on Alcohol, Drugs and Traffic Safety (ICADTS), and featuring the 2008 (2007)
  • Author(s): Marumo A., Kumazawa T., Lee X.-P., Hasegawa C., Suzuki O., Sato K.
  • Organizer: 8th Ignition Interlock Symposium (IIS)
  • Place of Presentation: Seattle, USA.