Risk analysis for gastric cancer before and after Helicobacter pylori eradication therapy by way of change in RUNX3 expression

• Project/Area Number: 19590730
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: KONISHI Hideyuki (2008) Kyoto Prefectural University of Medicine, 医学研究科, 助教 (30295670); 光藤 章二 (2007) Kyoto Prefectural University of Medicine, 医学研究科, 講師 (20229751)
• Co-Investigator(Kenkyū-buntansha): WAKABAYASHI Naoki 京都府立医科大学, 医学研究科, 講師 (20305577) ; SAKAKURA Chohei 京都府立医科大学, 医学研究科, 准教授 (10285257) ; 小西 英幸 京都府立医科大学, 医学研究科, 助教 (30295670)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: RUNX3 / Helicobacter pylori / 除菌療法 / 胃癌

Research Abstract

H. pylori除菌前後の胃粘膜のRUNX3発現を免疫染色で検討すると、除菌後短期間の観察では改善しなかったが、除菌後長期間では改善した。Methylation specific PCRでRUNX3の発現を評価すると、除菌後短期間の観察でも回復を示す症例があり、疾患別の差が推察された。疾患別にRUNX3の発現回復の程度を検討すると、胃癌既往群(早期胃癌内視鏡的治療術後)は良性潰瘍群に比べて回復し難く、また潰瘍群の中でも十二指腸潰瘍群で胃潰瘍群に比べ回復しやすかった。これらの結果から、除菌後のRUNX3の回復は胃癌発癌リスクのバイオマーカーになることが予測された。

Research Products

• [Journal Article] Recovery of RUNX3 Expression in Long- term Follow-Up after H. Pylori Eradication (2009)
  • Author(s): Tachibana S, Konishi H,Motoyoshi T, SakakuraC, Wakabayashi N, Mitsufuji S, Kataoka K.
  • Journal Title: Gastroetnerology 136
  • Peer Reviewed
• [Journal Article] 胃過形成性ポリープに対するH. pylori除菌治療-除菌効果と長期成績- (2009)
  • Author(s): 光藤章二、小西英幸、若林直樹
  • Journal Title: 消化器科 48 Pages: 18-23
  • Peer Reviewed
• [Journal Article] 除菌治療前後のH. pylori感染胃炎の変化-RUNX3の発現を中心に-消化器科 (2009)
  • Author(s): 立花俊治、小西英幸、阪倉長平
  • Journal Title: 48
• [Journal Article] 胃過形成性ポリープに対するH. pylori除菌治療-除菌効果と長期成績- (2009)
  • Author(s): 光藤章二, 小西英幸, 若林直樹
  • Journal Title: 消化器科 48 Pages: 18-23
• [Journal Article] 除菌治療前後のH. pylor感染胃炎の変化-RUNX3の発現を中心に- (2009)
  • Author(s): 立花俊治, 小西英幸, 阪倉長平
  • Journal Title: 消化器病学シンポー原点から未来への情報発信一第94回日本消化器病学会総会記念誌I消化管領域 Pages: 442-444
• [Journal Article] Recovery of RUNX3 Expression in Long-term Follow-Up after H. Pylori Eradication (2008)
  • Author(s): Tachibana S, Konishi H, Motoyoshi T, Sakakura C, Wakabayashi N, Mitsufuji S, Kataoka K.
  • Journal Title: Gastroetnerology 134
  • Peer Reviewed
• [Journal Article] IL-17 involved Helicobacterpylori-induced Gastric Inflammatory Responses in a Mouse Model (2008)
  • Author(s): Shiomi S, Toriie A, Imamura S, Konishi H, Mitsufuji S, Iwakura Y, Yamaoka Y, Ota H, Yamamoto T, Imanishi J, Kita M.
  • Journal Title: Helicobacter 13 Pages: 518-524
  • Peer Reviewed
• [Journal Article] Recovery of RUNX3 Expression in Long-Term Follow-Up after H. Pylori Eradication (2008)
  • Author(s): Tachibana S, T, Konishi H, Motoyoshi, Sakakura C, Wakabayashi N, Mitsufuji S, Kataoka K.
  • Journal Title: Gastroetnerology 134
  • Peer Reviewed
• [Journal Article] IL-17 is involved in Helicobacter pylori-induced Gastric Inflammatory Responses in a Mouse Model. 13 : 518-524, 2008. (2008)
  • Author(s): Shiomi S, Toriie A, Imamura S, Konishi H, Mitsufuji S, Iwakura Y, Yamaoka Y, Ota H, Yamamoto T, Imanishi J, Kita M.
  • Journal Title: Helicobacter 13 Pages: 518-52
  • Peer Reviewed
• [Journal Article] Frequent silencing of RUNX3 in esophageal squamous cell carcinomas is associated with radioresistance and poor prognosis (2007)
  • Author(s): Sakakura C, Miyagawa K, Fukuda KI, Nakashima S, Yoshikawa T, Kin S, Nakase Y, Ida H, Yazumi S, Yamagishi H, Okanoue T, Chiba T, Ito K, Hagiwara A, Ito Y.
  • Journal Title: Oncogene 26(40) Pages: 5927-5938
  • Peer Reviewed
• [Journal Article] Possible Involvement of RUNX3 Silencing in the Peritoneal Metastases of Gastric Cancers (2005)
  • Author(s): Sakakura C, Hasegawa K, Miyagawa K, Nakashima S, Yoshikawa T, Kin S, Nakase Y, Yazumi S, Yamagishi H, Okanoue T, Chiba T, Hagiwara A.
  • Journal Title: Clin Cancer Res 11(18) Pages: 6479-6488
  • Peer Reviewed
• [Journal Article] Frequent downregulation of the runt domain transcription factors RUNX1, RUNX3 and their cofactor CBFB in gastric cancer (2005)
  • Author(s): Sakakura C, Hagiwara A, Miyagawa K, Nakashima S, Yoshikawa T, Kin S, Nakase Y, Ito K, Yamagishi H, Yazumi S, Chiba T, Ito z
  • Journal Title: Int J Cancer 113(2) Pages: 221-228
  • Peer Reviewed
• [Journal Article] Frequent loss of RUNX3 gene expression in remnant stomach cancer and adjacent mucosa with special reference to topography (2005)
  • Author(s): Nakase Y, Sakakura C, Miyagawa K, Kin S, Fukuda K, Yanagisawa A, Koide K, Morofuji N, Hosokawa Y, Shimomura K, Katsura K, Hagiwara A, Yamagishi H, Ito K, Ito Y.
  • Journal Title: Br J Cancer 92(3) Pages: 562-569
  • Peer Reviewed
• [Journal Article] Bae SC.RUNX3 suppresses gastric epithelial cell growth by inducing p21(WAF1/Cip1) expression in cooperation with transforming growth factor {beta}-activated SMAD (2005)
  • Author(s): Chi XZ, Yang JO, Lee KY, Ito K, Sakakura C, Li QL, Kim HR, Cha EJ, Lee YH, Kaneda A, Ushijima T, Kim WJ, Ito Y
  • Journal Title: Mol Cell Biol 25(18) Pages: 8097-8107
  • Peer Reviewed
• [Journal Article] Expression and localization of Reg IV in human neoplastic and non-neoplastic tissues : Reg IV expression is associated with intestinal and neuroendocrine differentiation in gastric adenocarcinoma (2005)
  • Author(s): Oue N, Mitani Y, Aung PP, Sakakura C, Takeshima Y, Kaneko M, Noguchi T, Nakayama H, Yasui W.
  • Journal Title: J Pathol 207(2) Pages: 185-198
  • Peer Reviewed
• [Journal Article] Frequent loss of RUNX3 gene expression in human bile duct and pancreatic cancer cell lines (2004)
  • Author(s): Wada M, Yazumi S, Takaishi S, Hasegawa K, Sawada M, Tanaka H, Ida H, Sakakura C, Ito K, Ito Y, Chiba T.
  • Journal Title: Oncogene 23(13) Pages: 2401-2407
  • Peer Reviewed
• [Journal Article] Development of gastric carcinoma from intestinal metaplasia in Cdx2-transgenic mice (2004)
  • Author(s): Mutoh H, Sakurai S, Satoh K, Tamada K, Kita H, Osawa H, Tomiyama T, Sato Y, Yamamoto H, Isoda N, Yoshida T, Ido K, Sugano K.
  • Journal Title: Cancer Res 64(21) Pages: 7740-7747
  • Peer Reviewed
• [Journal Article] Runx3-/-gastric epithelial cells differentiate into intestinal type cells (2004)
  • Author(s): Fukamachi H, Ito K, Ito Y.
  • Journal Title: Biochem Biophys Res Commun 321(1) Pages: 58-64
  • Peer Reviewed
• [Journal Article] Causal Relationship between the Loss of RUNX3 Expression and Gastric Cancer (2002)
  • Author(s): Li QL, Ito K, Sakakura C, Fukamachi H, Inoue K, Chi XZ, Lee KY, Nomura S, Lee CW, Han SB, Kim HM, Kim WJ, Yamamoto H, Yamashita N, Yano T, Ikeda T, Itohara S, Inazawa J, Abe T, Hagiwara A, Yamagishi H, Ooe A, Kaneda A, Sugimura T, Ushijima T, Bae SC, Ito Y.
  • Journal Title: Cell 109(1) Pages: 113-124
  • Peer Reviewed
• [Presentation] Recovery of RUNX3 Expression in Long-term Follow-Up after H. pylori Eradication (2009)
  • Author(s): Tachibana S, Konishi H, Motoyoshi T, Sakakura C, Wakabayashi N, Mitsufuji S, Kataoka K
  • Organizer: Digestive Disease Week 2009
  • Place of Presentation: Chicago, USA
  • Year and Date: 2009-06-03
• [Presentation] Recovery of RUNX3 Expression in Long-term Follow-Up after H. pylori Eradication (2008)
  • Author(s): Tachibana S, Konishi H, Fukumoto K, Motoyoshi T, SakakuraC, Wakabayashi N, Yagi N, Naito Y, Yoshikawa T, Mitsufuji S, Kataoka K.
  • Organizer: Digestive Disease Week 2008
  • Place of Presentation: San Diego, USA
  • Year and Date: 2008-05-19
• [Presentation] Recovery of RUNX3 Expression in Long-Term Follow-Up after H. Pylori Eradication (2008)
  • Author(s): Tachibana S, T, Konishi H, Motoyoshi, Sakakura C, Wakabayashi N, Mitsufuji S, Kataoka K.
  • Organizer: Digestive Disease Week 2008
  • Place of Presentation: San Diego, USA
  • Year and Date: 2008-05-19
• [Presentation] 除菌治療前後のH. pylori感染胃炎の変化-RUNX3の発現を中心に- (2008)
  • Author(s): 立花俊治、小西英幸、阪倉長平
  • Organizer: 第94回日本消化器病学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2008-05-10
• [Presentation] 胃過形成性ポリープに対するH. pylori除菌治療-除菌効果と長期成績- (2008)
  • Author(s): 光藤章二、小西英幸、若林直樹
  • Organizer: 第94回日本消化器病学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2008-05-08
• [Presentation] 除菌治療前後のH. pylori感染胃炎の変化-RUNX3の発現を中心に (2007)
  • Author(s): 立花俊治、元好貴之、小西英幸、若林直樹、光藤章二、片岡慶正、岡上 武、阪倉長平
  • Organizer: DDW-Japan 2007 Kobe.
  • Place of Presentation: 神戸
  • Year and Date: 2007-10-18
• [Presentation] H. pylori除菌治療前後のRUNX3の発現変化 (2007)
  • Author(s): 立花俊治、小西英幸、元好貴之、若林直樹、光藤章二、片岡慶正、岡上 武、阪倉長平
  • Organizer: 第13回日本ヘリコバクター学会
  • Place of Presentation: 大津
  • Year and Date: 2007-06-22
• [Presentation] 除菌治療前後のH. pylori感染胃炎の変化-RUNX3の発現を中心に- (2007)
  • Author(s): 元好貴之、小西英幸、阪倉長平
  • Organizer: 第93回日本消化器病学会総会
  • Place of Presentation: 青森
  • Year and Date: 2007-04-19
• [Presentation] Molecular biological study for diffuse type gastric cancer-A view of Runx3 expression- (2007)
  • Author(s): Motoyoshi T, Konishi H, Tachibana S, Sakakura C, Wakabayashi N, Mitsufuji S, Kataoka K, Okanoue T.
  • Organizer: Digestive Disease Week 2007
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2007-04-19