Development of pancreatic cancer stem cell targeted therapy using cells derived from bone marrow

• Project/Area Number: 19590745
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Tohoku University
• Principal Investigator: SATOH Kennichi Tohoku University, 病院, 助教 (10282055)
• Co-Investigator(Kenkyū-buntansha): HIROTA Morihisa 東北大学, 病院, 助教 (70400364)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 膵癌 / 幹細胞 / 骨髄由来細胞

Research Abstract

我々は膵癌幹細胞を標的とした骨髄由来細胞による遺伝子治療を行うことを最終目的に、膵癌幹細胞の同定と、骨髄由来細胞をその特異的攻撃細胞として遺伝子を発現させるために基礎検討を行った。最近、上皮間葉形質転換(EMT)が誘導されている癌細胞と癌幹細胞の性質が類似していると報告されたことから、我々は膵癌のEMTを誘導するBMP4の下流の標的遺伝子であるMSX2とS100Pに着目して研究を行った。その結果、これらの分子を発現する癌細胞が膵癌幹細胞を多く含む可能性を示唆した。

Research Products

• [Journal Article] Expression of the calcium-binding protein S100P is regulated by bone morphogenetic protein in pancreatic duct epithelial cell lines (2009)
  • Author(s): Hamada, S, Satoh K ; Hirota M, Fujibuchi W, Kanno A ; Umino J ; Ito H ; Satoh A ; Kikuta K ; Kume, K ; Masamune A, Shimosegawa T
  • Journal Title: Cancer Sci 100 Pages: 103-10
  • NAID: 10025141512
  • Peer Reviewed
• [Journal Article] Expression of the calcium-binding protein S100P is regulated by bone morphogenetic protein in pancreatic duct epithelial cell lines (2009)
  • Author(s): Hamada S, Satoh K, Hirota M, Fujibuchi W, Kanno A, Umino J, Ito FI, Satoh A, Kikuta K, Kume K, Masamune A, Shimosegawa T
  • Journal Title: Cancer Sci 100 Pages: 103-110
  • NAID: 10025141512
  • Peer Reviewed
• [Journal Article] Up-Regulation of MSX2 Enhances the Malignant Phenotype and is Associated with Twist 1 Expression in Human Pancreatic Cancer Cells (2008)
  • Author(s): Satoh K, Hamada S, Kimura K, Kanno A, Hirota M, Umino J, Fujibuchi W, MasamuneA, Tanaka N, Miura K, Egawa S, Motoi F, Unno M, Vonderhaar BK, Shimosegawa T
  • Journal Title: Am J Pathol 172 Pages: 926-39
  • Peer Reviewed
• [Journal Article] Unno, M, Shimosegawa T. Periostin, secreted from stromal cells, has biphasic effect on cell migration and correlates with the epithelial to mesenchymal transition of human pancreatic cancer cells (2008)
  • Author(s): Kanno A, Satoh K, Masamune A, Hirota M, Kimura K, Umino J, Hamada S, Satoh A, Egawa S, Motoi F
  • Journal Title: Int J Cancer 122 Pages: 2707-2718
  • Peer Reviewed
• [Journal Article] Expression of Sonic hedgehog signaling pathway correlates with the tumorigenesis of intraductal papillary mucinous neoplasm of the pancreas (2008)
  • Author(s): Satoh K, Kanno A, Hamada S, Hirota M, Umino J, Masamune A, Egawa S, Motoi F, Unno M, Shimosegawa T
  • Journal Title: Oncol Rep 19 Pages: 1185-90
  • Peer Reviewed
• [Journal Article] Periostin, secreted from stromal cells, has biphasic effect on cell migration and correlates with the epithelial to mesenchymal transition of human pancreatic cancer cells (2008)
  • Author(s): Kanno A, Satoh K, Masamune A, Hirota M, Kimura K, Umino J, Hamada S, Satoh A, Egawa S, Motoi F, Unno M, Shimosegawa T
  • Journal Title: Int J Cancer 122 Pages: 2707-2718
  • Peer Reviewed
• [Journal Article] Up-Regulation of MSX2 Enhances the Malignant Phenotype and Is Associated with Twist 1 Expression in Human Pancreatic Cancer Cells. (2008)
  • Author(s): Satoh K, Hamada S, Kimura K, Kanno A, Hirota M, Umino J, Fujibuchi W, MasamuneA, Tanaka N, Miura K, Egawa S, Motoi F, Unno M, Vonderhaar BK, Shimosegawa T.
  • Journal Title: Am J Pathol 172 Pages: 926-939
  • Peer Reviewed
• [Journal Article] Activation of Notch signaling in tumorigenesis of experimental pancreatic cancer induced by dimethylbenzanthracene in mice (2007)
  • Author(s): Kimura K, Satoh K, Kanno A, Hamada S, Hirota M, Endoh M, Masamune A, Shimosegawa T
  • Journal Title: Cancer Sci 98 Pages: 155-162
  • Peer Reviewed
• [Journal Article] Bone morphogenetic protein 4 induces epithelial-mesenchymal transition through MSX2 induction on pancreatic cancer cell line (2007)
  • Author(s): Hamada S, Satoh K, Hirota M, Kimura K, Kanno A, Masamune A, Shimosegawa T
  • Journal Title: J Cell Physiol 213 Pages: 768-74
  • Peer Reviewed
• [Journal Article] Activation of Notch signaling in tumorigenesis of experimental pancreatic cancer induced by dimethylbenzanthracene in mice. (2007)
  • Author(s): Kimura K, Satoh K, Kaimo A, Hamada S, Hirota M, Endoh M, Masamune A, Shimosegawa T
  • Journal Title: Cancer Sci 98 Pages: 155-162
  • Peer Reviewed
• [Journal Article] Bone morphogenetic protein 4 induces epithelial-mesenchymal transition throughMSX2 induction on pancreatic cancer cell line. (2007)
  • Author(s): Hamada S, Satoh K, Hirota M, Kimura K, Kanno A, Masamune A, Shimosegawa T.
  • Journal Title: J Cell Physiol 213 Pages: 768-74
  • Peer Reviewed
• [Presentation] 膵癌細胞株におけるBMP4によるカルシウム結合蛋白S100Pの発現誘導とその機能についての検討 (2008)
  • Author(s): 濱田晋、佐藤賢一、廣田衛久、菅野敦、海野純、伊藤広通、下瀬川徹
  • Organizer: 第50回日本消化器病学会大会
  • Place of Presentation: 東京
  • Year and Date: 2008-10-03
• [Presentation] MSX2 promotes epithelial mesenchymal transition and associates with Twist 1 expression in human pancreatic cancer cells (2007)
  • Author(s): Kennichi Satoh, Shin Hamada, Kenji Kimura, Atsushi Kanno, Morihisa Hirota, Tooru Shimosegawa
  • Organizer: DIGESTIVE DISEASE WEEK 2007
  • Place of Presentation: Washington DC, U.S.A
  • Year and Date: 2007-05-21
• [Presentation] MSX2 promotes epithelial mesenchymal transition and associates with Twist 1 expression in human pancreatic cancer cells. (2007)
  • Author(s): Kennichi Satoh, Shin Hamada, Kenji Kimura, Atsushi Kanno, Morihisa Hirota, and Tooru Shimosegawa.
  • Organizer: DIGESTIVE DISEASE WEEK 2007
  • Place of Presentation: Washington DC
  • Year and Date: 2007-05-21