Hepatocyte differentiation aiming at transplantation of ES cells for hepatic insufficiency

• Project/Area Number: 19590748
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Chiba University
• Principal Investigator: TOMIZAWA Minoru Chiba University, 医学部・附属病院, 助教 (90334193)
• Co-Investigator(Kenkyū-buntansha): OHIRA Miki 千葉県がんセンター(研究所), 臨床ゲノムセンター, 研究員 (20311384)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 肝細胞分化 / シグナル伝達 / 発生 / 再生医学 / 肝細胞分

Research Abstract

肝不全への移植療法への応用を目指し、胚性幹(ES)細胞より肝細胞を分化・製造する方法を研究した。ブドウ糖は細胞の生存に必須である。ガラクトースは肝細胞のみがエネルギーに変換し、生存可能である。そこでブドウ糖を除き、ガラクトースを添加した培地でES細胞を培養したところ、肝芽細胞への分化が促進され、肝芽細胞が高率に収穫されることを明らかになった。

Research Products

• [Journal Article] Hepatoblast-like cells enriched from mouse embryonic stem cells in a medium without glucose, pyruvate, arginine and tyrosine (2008)
  • Author(s): Tomizawa M., (7名)
  • Journal Title: Cell Tissue Res. 333 Pages: 17-27
  • Peer Reviewed
• [Journal Article] Picropodophyllin suppresses proliferation and invasion of hepatocellular carcinoma under serum starvation (2008)
  • Author(s): Tomizawa M., (1 名)
  • Journal Title: Mol. Med. Rep. 1 Pages: 685-688
  • Peer Reviewed
• [Journal Article] Hepatoblast-like cells enriched from mouse embryonic stem cells in a medium without glucose, pyruvate, arginine and tyrosine. (2008)
  • Author(s): Tomizawa M
  • Journal Title: Cell Tissue Res. 333 Pages: 17-27
  • Peer Reviewed
• [Journal Article] Picropodophyllin suppresses proliferation and invasion of hepatocellular carcinoma under serum starvation. (2008)
  • Author(s): Tomizawa M
  • Journal Title: Mol. Med. Rep. 1 Pages: 685-688
  • Peer Reviewed
• [Presentation] 肝細胞分化を促進する分子生物学的メカニズムの解明 (2007)
  • Author(s): 富澤 稔
  • Organizer: 第11回目本肝臓学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2007-10-18
• [Patent(Industrial Property Rights)] (2007)
  • Inventor(s): 富澤稔
  • Industrial Property Rights Holder: 千葉大学
  • Industrial Property Number: 2007-277285
  • Filing Date: 2007-10-15
  • Overseas
• [Patent(Industrial Property Rights)] 特許権 (2007)
  • Inventor(s): 富澤 稔
  • Industrial Property Rights Holder: 千葉大学
  • Industrial Property Number: 2007-277285
  • Filing Date: 2007-10-25