Charactrization of a single nucleotide polymorphism that determine the capacity of fatty acid beta-oxidation and its 3D-conformation
| Project/Area Number |
19590772
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kochi University |
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Principal Investigator |
SAIBARA Toshiji Kochi University, 教育研究部・医療学系, 准教授 (60145125)
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| Co-Investigator(Kenkyū-buntansha) |
TODA Katsumi 高知大学, 教育研究部・医療学系, 准教授 (40197893)
TAKAHASHI Masaya 高知大学, 教育研究部・医療学系, 助教 (80380335)
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| Project Period (FY) |
2007 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 脂肪酸β酸化 / 遺伝子多型 / リパーゼ / 結晶解析 / Fatty acid / Beta-oxidation / PPAR / Crystalography |
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| Research Abstract |
脂肪酸β酸化の律速段階を制御する遺伝子とその機能性遺伝子多型を特定し、脂肪酸のβ酸化能をin vivoで測定するために必要な手法を開発し、遺伝子多型に応じて肝臓における脂肪酸β酸化能に個体差が生じることも明らかにした。そこで野生型および変異型蛋白質を遺伝子組み換えにより作成した。これに反応する抗体を作成したところ、両者ともWesaternblotで単一バンドとして検出され、細胞内では2量体として存在することが明らかになった。
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Report
(3 results)
Research Products
(56 results)
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[Journal Article] Mutations in the small heterodimer partner gene increase morbidity risk in Japanese type 2 diabetes patients2008
Author(s)
Enya M, Horikawa Y, Kuroda E, Yonemaru K, Tonooka N, Tomura H, Oda N, Yokoi N, Yamagata K, Shihara N, Iizuka K, Saibara T, Seino S, Takeda J
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] The phosphatidyl ethanolamine N-methyltransferase gene V175M single nucleotide polymorphism confers the susceptibility to NASH in Japanese population2007
Author(s)
Dong H, Wang J, Li C, Hirose A, Nozaki Y, Takahashi M, Ono M, Akisawa N, Iwasaki S, Saibara T, Onishi S.
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Journal Title
J Hepatol 46
Pages: 915-920
Related Report
Peer Reviewed
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[Journal Article] Angiotensin II type 1 receptor blocker inhibits fibrosis in rat nonalcoholic steatohepatitis2007
Author(s)
Hirose A, Ono M, Saibara T, Nozaki Y, Masuda K, Yoshioka A, Takahashi M, Akisawa N, Iwasaki S, Oben JA, Onishi S.
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Journal Title
Hepatology 45
Pages: 1375-81
Related Report
Peer Reviewed
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[Presentation] Super-paramagnetic iron oxide-MRI : A novel technique in experimental liver studies shows defective Kupffer cell phagocytic function in human non alcoholic fatty liver disease2008
Author(s)
Ono M, Kubota K, Hirose A, Nozaki Y, Takahashi M, Iwasaki S, Saibara T, Ogawa Y, Onishi S, Oben JA.
Organizer
European Association for the Study of Liver Disease
Place of Presentation
Milan, Italy
Year and Date
2008-04-24
Related Report
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[Presentation] Methionine-choline metabolism is involved in the pathogenesis of nonalcoholic steatohepatitis2008
Author(s)
Tokumitsu K, Ogasawara K, Maki M, Hirose A, Nozaki Y, Takahashi M, Ono M, Iwasaki S, Saibara T, Onishi S.
Organizer
European Association for the Study of Liver Disease
Place of Presentation
Milan, Italy
Year and Date
2008-04-24
Related Report
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