Therapeutic strategies with chimeric oncolytic adenoviruses and a cell-mediated delivery system for gastrointestinal solid tumors

• Project/Area Number: 19590798
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Chiba Cancer Center (Research Institute)
• Principal Investigator: KAWAMURA Kiyoko Chiba Cancer Center (Research Institute), 病理研究部, 主席研究員 (80260248)
• Co-Investigator(Kenkyū-buntansha): TAGAWA Masatoshi 千葉県がんセンター, 病理研究部, 部長 (20171572) ; 山口 武人 千葉県がんセンター(研究所), 医療局, 診療部長 (00241969)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 膵臓がん / ウイルス / がん治療 / 細胞傷害活性 / 転写調節 / 膵がん / アデノウイルス / E1A遺伝子 / CD46 / 抗がん剤 / MDM-7 / ファイバー・ノブ領域 / インターロイキン24 / MDA-7 / EIA遺伝子

Research Abstract

難治性の消化器固形腫瘍に対する治療成績の向上は、癌治療における大きな課題の一つである。そこで生体の免疫応答を抑制しがちな従来の化学療法、放射線療法とは異なり、その可能性のないアデノウイルスを用いた殺細胞効果を検討した。
すなわち、細胞傷害活性を有する同ウイルスの増殖を腫瘍細胞特異的に惹起し、正常細胞を傷害せず、腫瘍のみに細胞死を誘導しようとするものである。
また腫瘍選択的な抗腫瘍効果をウイルスの改変で増強しうるかどうか、さらに抗がん剤との併用効果の有無についても検討した。

Research Products

• [Journal Article] Cancer therapy with local oncolysis and topical cytokine secretion (2008)
  • Author(s): Tagawa, M., Kawamura, K., Ueyama, T., Nakamura, M., Tada, Y., Ma, G., Li, Q., Suzuki, N., Shimada, H., Kuriyama, T. and Ochiai, T
  • Journal Title: Front. Biosci 13 Pages: 2578-2587
  • Peer Reviewed
• [Journal Article] Transcriptional regulatory regions of the survivin gene activate an exogenous suicide gene in human tumors and enhance the sensitivity to a prodrug (2007)
  • Author(s): Kawamura, K., Yu, L., Tomizawa, M., Shimozato, O., Ma, G., Li, Q., Sato, A., Yang, Y., Suzuki, T., Abdel-Azizi, N. M., Tagawa, M
  • Journal Title: Anticancer Res 27 Pages: 89-94
  • Peer Reviewed
• [Journal Article] Adenovirus type 5 substituted with type 11 or type 35 fiber structure increases its infectivity to huma cells and enable dualgene transfer in CD46-dependent and -independent manners (2007)
  • Author(s): Yu, L., Shimozato, O., Li, Q., Kawamura, K., Ma, G., Namba, M., Ogawa, T., Kaiho, I. And Tagawa, M
  • Journal Title: Antican
• [Journal Article] Transcriptional regulatory regions of the survivin gene activate an exogenous suicide gene human tumors and enhance the sensitivity to a prodrug. (2007)
  • Author(s): Kawamura, K
  • Journal Title: Anticancer Res. 27 Pages: 89-94
  • Peer Reviewed
• [Journal Article] Adenovirus type 5 substituted with type 11 or type 35 fiber structure increases its infectivity to human cells snd enables dual gene transfer in CD46-dependent and-independent manners. (2007)
  • Author(s): Yu, L
  • Journal Title: Anticancer Res. 27 Pages: 2311-2316
  • Peer Reviewed
• [Journal Article] Cytotoxicity of adenoviruses expressing the wild-type p53 gene to esophageal carcinoma cells is linked with the CAR expression level and indirectly with the endogenous
  • Author(s): Ma, G., Kawamura, K., Li, Q., Suzuki, N., Liang, M., Namba, M., Shimada, H. and Tagawa, M
  • Journal Title: Cancer Gene Ther (in press) Pages: 53-53
  • Peer Reviewed
• [Journal Article] Cytotoxicity of adenoviruses expressing the wild-type p53 gene to esophageal carcinoma cells is linked with the CAR expression level and indirectly with the endogenous p53 status
  • Author(s): Ma G
  • Journal Title: Cancer Gene Ther (印刷中)
  • Peer Reviewed
• [Presentation] Cytotoxicity of Ad bearing the wt p53 to esophageal cancer is linked with the endogenous p53 status and CAR expression (2009)
  • Author(s): 馬 光宇、川村希代子、李 全海、鈴木健夫、佐藤彩子、張 正茂、鈴木信夫、島田英昭、落合武徳
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-10-04
• [Presentation] アデノウイルスによる腫瘍細胞死に対して影響を与える細胞因子の検討 (2008)
  • Author(s): 川村希代子、馬 光宇、李 全海、植山太郎、廣島健三、島田英昭、多田裕司、滝口裕一、巽 浩一郎,栗山喬之、田川雅敏
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-10-28
• [Presentation] Replication-competent adenoviruses-medated tumor cell death is influenced by host-derived factors (2008)
  • Author(s): Kawamura, K., Ma, G., Li, Q., Ueyama, T., Takei, Y., Tada, Y., Takiguchi, Y., Tatsumi, T., Suzuki, N., Yamaguchi, N., Hiroshima, K., Shimada
  • Organizer: International Society for Cell and Gene Therapy of Cancer 2008
  • Place of Presentation: Shijarzhuang, China
  • Year and Date: 2008-09-21
• [Presentation] Replication-competent adenoviruses-mediated tumor cell death is influenced by host-derived factors (2008)
  • Author(s): 川村希代子
  • Organizer: International Society for Cell and Gene Therapy of Cancer 2008
  • Place of Presentation: 石家庄市、中国
  • Year and Date: 2008-09-20
• [Presentation] Cytotoxicity of Ad bearing the wt p53 to esophageal cancer is linked with the endogenous p53 status and CAR expression. (2007)
  • Author(s): 馬 光宇
  • Organizer: 第66回日本癌学会学術総云
  • Place of Presentation: 横浜市
  • Year and Date: 2007-10-04
• [Remarks]
  • URL: http://www.chiba-cc.jp/inst/jp/index.html