To detect non-coding RNA related to heart failure

• Project/Area Number: 19590808
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Fujita Health University (2008); Nagoya University (2007)
• Principal Investigator: HIDEO Izawa Fujita Health University, 医学部, 准教授 (80402569)
• Co-Investigator(Kenkyū-buntansha): CHENG Xian Wu 名古屋大学, 医学部, 寄付講座講師 (30378228) ; YOKOTA Mitsuhiro 愛知学院大学, 歯学部, 教授 (50201851) ; NODA Akiko 名古屋大学, 医学部, 助教 (80252287) ; NAGATA Kohzo 名古屋大学, 医学部, 准教授 (20378227) ; MUROHARA Yotoaki 名古屋大学, 大学院・医学系研究科, 教授 (90299503)
• Co-Investigator(Renkei-kenkyūsha): YOKOTA Mitsuhiro 愛知学院大学, 歯学部, 教授 (50201851) ; NODA Akiko 名古屋大学, 医学部, 助教 (80252287) ; NAGATA Kohzo 名古屋大学, 医学部, 准教授 (20378227) ; MUROHARA Yotoaki 名古屋大学, 大学院・医学系研究科, 教授 (90299503)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 心臓病態学 / 心不全 / 機能性RNA / 心筋症 / ドブタミン負荷試験

Research Abstract

本研究の目的は、不全心筋の病態形成に影響する機能性RNAを同定することである。我々は心筋症症例から得た心筋生検標本におけるmRNAの定量解析、病態形成に関連するmiRNAの網羅的探索を行った。不全心筋における交感神経刺激伝達系および心筋細胞内Ca動態関連タンパクの発現異常と心筋収縮予備能との関連を見出し、さらに、心筋収縮予備能異常に関連している複数個のmiRNAを同定した。また、肥大型心筋症における心筋収縮予備能とミトコンドリア異常との関連も明らかにした。

Research Products

• [Journal Article] Dobutamine stress testing as a diagnostic tool for evaluation of myocardial contractiMle reserve in asymptomatic or mildly symptomatic patients with dilated cardiomyopathy (2008)
  • Author(s): Kobayashi M, Izawa H, Cheng XW, Asano H, Hirashiki A, Unno K, Ohshima S, Yamada T, Murase Y, Kato TS, Obata K, Noda A, Nishizawa T, Isobe S, Nagata K, Matsubara T, urohara T, Yokota M.
  • Journal Title: JACC Cardiovasc Imaging 1(6) Pages: 718-26
  • Peer Reviewed
• [Journal Article] Superoxide-dependent cathepsin activation is associated with hypertensive myocardial remodeling and represents a target for angiotensin II type 1 receptor blocker treatment (2008)
  • Author(s): Cheng XW, Murohara T, Kuzuya M, Izawa H, Sasaki T, Obata K, Nagata K, Nishizawa T, Kobayashi M, Yamada T, Kim W, Sato K, Shi GP, Okumura K, Yokota M.
  • Journal Title: Am J Pathol 173(2) Pages: 358-69
  • Peer Reviewed
• [Journal Article] Dobutamine stress testing as a diagnostic tool for evaluaticn of myxardial ocntractile resarve in asymptomstic or mildly symptomatic patients with dilated cardiomyopathy (2008)
  • Author(s): Kobayashi M
  • Journal Title: J Am Coll Cardiol Img 1 Pages: 718-726
  • Peer Reviewed
• [Journal Article] Superoxide-dependent cathepsin activaticn is associated with hypertensive myocardial remodeling and represent sa target for angioteisin II type 1 receptor blocker treatment。 (2008)
  • Author(s): Cheng xw
  • Journal Title: Am J Pathol 173 Pages: 358-369
  • Peer Reviewed
• [Presentation] Relation of Functional and Morphological Changes in Mitochondria to Myocardial Contractile and Relaxation Reserves in Asymptomatic to Mildly Symptomatic Patients with Hypertrophic Cardiomyopathy. (2008)
  • Author(s): Unno K, Izawa H, et.al.
  • Organizer: American Heart Association, Scientific Sessions 2008
  • Place of Presentation: ニューオーリンズ(米国)
  • Year and Date: 2008-11-11
• [Presentation] Relation of Functional and Morphological Changes in Mitochondria to Myocardial Contractile and Relaxation Reserves in Asymptomatic to Mi ldly Symptomatic Patients with Hypertrophic Cardiomyopathy (2008)
  • Author(s): Unno K
  • Organizer: Scientific Sessions 2008, American Heart Association
  • Place of Presentation: ニューオーリンズ(米国)
  • Year and Date: 2008-11-11
• [Presentation] Reduced [123I] metaiodobenzylguanidine Uptake Predicts Impaired Adrenergic Myocardial Functional Reserve in Asymptomatic or Mildly Symptomatic Patients with Idiopathic Dilated Cardiomyopathy (2008)
  • Author(s): Kobayashi M, Izawa H, et.al.
  • Organizer: American Heart Association, Scientific Sessions 2008
  • Place of Presentation: ニューオーリンズ(米国)
  • Year and Date: 2008-11-09
• [Presentation] Reduced[123I]metaiodobenzylguanidine Uptake Predicts Impaired Adren ergic Myocardial Functional Reserve in Asymptomatic or Mildly Sympto matic Patients with Idiopathic Dilated Cardiomyopathy (2008)
  • Author(s): Kobayashi M
  • Organizer: Scientific Sessions 2008, American Heart Association
  • Place of Presentation: ニューオーリンズ(米国)
  • Year and Date: 2008-11-09
• [Presentation] Dobutamine-induced mechanical alternans predicts poor prognosis in mildly to moderately symptomatic patients with idiopathic dilated cardiomyopathy in sinus rhythm (2007)
  • Author(s): Hirashiki A, Izawa H, et.al.
  • Organizer: American Heart Association, Scientific Sessions 2007
  • Place of Presentation: オーランド(米国)
  • Year and Date: 2007-11-06
• [Presentation] Dobutamine-induced mechanical alternans predicts poor prognosis in mildly to moderately symptomatic patients with idiopathic dilated cardiomy opathy in sinus rhythm (2007)
  • Author(s): Hirashiki A, Izawa H, et. al.
  • Organizer: American Heart Association, Scientific Sessions 2007
  • Place of Presentation: Orlando, Florida, USA
  • Year and Date: 2007-11-06