Genetic screening of cardiac regeneration in zebrafish and medakafish

• Project/Area Number: 19590832
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Keio University
• Principal Investigator: MAKINO Shinji Keio University, 医学部, 准教授 (20306707)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 心筋再生 / 小型魚類 / 順遺伝学 / プロテオグリカン / メダカ / 心室低形成 / ポジショナルクローニング

Research Abstract

小型魚類は哺乳動物には認められない再生能力がある。化学変異剤により作製した変異体を使って、再生現象を担う遺伝子を発見するためのスクリーニングにおいて、心室形成不能メダカ変異体を得た。この変異体は発生過程において心室筋細胞増殖能力を失っており、心室形成ができずに胎生致死であった。この変異体のポジショナルクローニングをおこなった結果、心筋特異的な細胞外マトリックスに遺伝子変異が存在することを発見した。

Research Products

• [Journal Article] Reversible whole-organism cell cyclearrest in a living vertebrate (2009)
  • Author(s): Sampetrean O, Iida S, Makino S, Matsuzaki Y, Ohno K, Saya H
  • Journal Title: Cell Cycle 15;8(4) Pages: 620-7
  • Peer Reviewed
• [Journal Article] A novel in vitro system for studying cardiomyocyte differentiation with medaka embryonic cells (2009)
  • Author(s): Hyodo M, Makino S, Awaji Y, SakuradaY, Ohkubo T, Murata M, Fukuda K, Tsuda M
  • Journal Title: Int J Dev Biol 23
  • Peer Reviewed
• [Journal Article] A novel in vitro system for studying cardiomyocyte differentiation with medaka embryonic cells. (2009)
  • Author(s): Hyodo M, Makino S, Awaji Y, Sakurada Y, Ohkubo T, Murat a M, Fukuda K, Tsuda M.
  • Journal Title: Int J Dev Biol. In Press
  • Peer Reviewed
• [Journal Article] Reversible whole-organism cell cycle arrest in a living vertebrate. (2009)
  • Author(s): Sampetrean O, Iida S, Makino S, Matsuzaki Y, Ohno K, Saya H.
  • Journal Title: Cell Cycle. 8 Pages: 620-627
  • Peer Reviewed
• [Journal Article] Common marmoset embryonic stem cell can differentiate into cardiomyocytes (2008)
  • Author(s): Chen H, Hattori F, Murata M, Li W, Yuasa S, Onizuka T, Shimoji K, Ohno Y, Sasaki E, Kimura K, Hakuno D, Sano M, Makino S, Ogawa S, Fukuda K
  • Journal Title: Biochem Biophys Res Commun 369(3) Pages: 801-806
  • Peer Reviewed
• [Journal Article] Inhalation of hydrogen gas reducesinfarct size in the rat model of myocardial ischemia-reperfusioninjury (2008)
  • Author(s): Hayashida K, Sano M, Ohsawa I, Shinmura K, Tamaki K, Kimura K, EndoJ, Katayama T, Kawamura A, Kohsaka S, Makino S, Ohta S, Ogawa S, Fukuda K
  • Journal Title: Biochem Biophys Res Commun 373(1) Pages: 30-35
  • Peer Reviewed
• [Journal Article] Common marmoset embryonic stem cell can differentiate into cardiomyocytes. (2008)
  • Author(s): Chen H, Hattori F, Murata M, Li W, Yuasa S, Onizuka T, Shimoji K, Ohno Y, Sasaki E, Kimura K, Hakuno D, Sano M, Makino S, Ogawa S, Fukuda K.
  • Journal Title: Biochem Biophys Res Commun. 369 Pages: 801-806
  • Peer Reviewed
• [Journal Article] Inhalation of hydrogen gas reduces infarct size in the rat model of myocardial ischemia-reperfusion injury. (2008)
  • Author(s): Hayashida K, Sano M, Ohsa wa I, Shinmura K, Tamaki K, Kimura K, Endo J, Katayama T, Kawamura A, Kohsaka S, Makino S, Ohta S, Ogawa s, Fukuda K.
  • Journal Title: Biochem Biophys Res Commun. 373 Pages: 30-35
  • Peer Reviewed
• [Journal Article] Sema3a maintainsnormal heart rhythm throughsympathetic innervation patterning (2007)
  • Author(s): Ieda M, Kanazawa H, Kimura K, HattoriF, Ieda Y, Taniguchi M, Lee JK, Matsumura K, Tomita Y, Miyoshi S, Shimoda K, Makino S, Sano M, Kodama I, Ogawa S, Fukuda K
  • Journal Title: Nat Med 13 Pages: 604-612
  • Peer Reviewed
• [Journal Article] Intramolecular control of protein stability, subnuclear compartmentalization, and coactivator function of peroxisome proliferator-activated receptorgamma coactivator lalpha (2007)
  • Author(s): Sano M, Tokudome S, Shimizu N, Yoshikawa N, Ogawa C, Shirakawa K, Endo J, Katayama T, Yuasa S, Ieda M, Makino S, Hattori F, Tanaka H, Fukuda K
  • Journal Title: J Biol Chem 282(35) Pages: 25970-25980
  • Peer Reviewed
• [Journal Article] Bonemarrow derived cells are involved in the pathogenesis of cardiac hypertrophy in response to pressure overload (2007)
  • Author(s): Endo J, Sano M, Fujita J, Hayashida K, Yuasa S, Aoyama N, Takehara Y, Kato O, Makino S, Ogawa S, Fukuda K
  • Journal Title: Circulation 116(10) Pages: 1176-1184
  • Peer Reviewed
• [Journal Article] Sema3a maintains normal heart rhythm through sympathetic innervation patterning (2007)
  • Author(s): Masaki leda, Shinii Makino, et. al.
  • Journal Title: Nat Med 13 Pages: 604-612
  • Peer Reviewed
• [Journal Article] Intramolecular control of protein stability,subnuclear compartmentalization,and coactivator function of peroxisome proliferator-activated receptor gamma coactivator lalpha (2007)
  • Author(s): Motoaki Sano, Shinii Makino, et. al.
  • Journal Title: J Biol Chem 282 Pages: 25970-25980
  • Peer Reviewed
• [Journal Article] Bone marrow derived cells are involved in the pathogenesis of cardiac hypertrophy in response to pressure overload (2007)
  • Author(s): Jin Endo, Shinji Makino, et. al.
  • Journal Title: Circulation 116 Pages: 1176-1184
  • Peer Reviewed
• [Presentation] Cardiomyocytes Derived from Bimaternal Parthenogenetic ES Cells by Epigenetic Modification have a Different Property and Genetic Presentation in Cardiogenesis (2009)
  • Author(s): Toshimi Kageyama, Shinji Makino, Tomoyuki Tokunaga, Tomohiro Kono Keiichi Fukuda
  • Organizer: 日本循環器学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-03-22
• [Presentation] Versican is Essential for ventricular Trabeculation and outflow Tract Formation in Medaka Heart (2009)
  • Author(s): Sung Han Yoon, Shinji Makino, Satoshi Ogawa
  • Organizer: 日本循環器学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-03-21
• [Presentation] Yes-associated Protein(YAP) is Essential for the Linear Heart Tube Formation in Cardiac Development (2009)
  • Author(s): Yuta Higashikuse, Shinji Makino, Keiichi Fukud
  • Organizer: 日本循環器学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-03-20
• [Presentation] Genetic approach forcardiac regeneration using zebrafish & medakafish (2008)
  • Author(s): Shinji Makino
  • Organizer: The 6th Korea-Japan Joint Symposium on Vascular Biology and Medicine
  • Place of Presentation: 金沢
  • Year and Date: 2008-12-08
• [Presentation] Genetic approach for cardiac regeneration using zebrafish & medakafish (2008)
  • Author(s): Makino S
  • Organizer: The 6th Korea-Japan Joint Symposium on Vaseular Biology and Medicine
  • Place of Presentation: 金 沢
  • Year and Date: 2008-12-08
• [Presentation] Imprinting gene-modified parthenogenic ES cells can be a novel autologous cell source for generating regenerative cardiomyocytes (2008)
  • Author(s): Kageyama T, Makino S, Fu kuda K, et al.
  • Organizer: American Heart Association 2008
  • Place of Presentation: New Orleans (U.S.A.)
  • Year and Date: 2008-11-11
• [Presentation] 小型魚類を用いた心臓発生、再生研究 (2008)
  • Author(s): Makino S
  • Organizer: 第16回プロテオグリカンフォーラム
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2008-09-13
• [Presentation] Activin A can Induce the Differentiation of Cardiomyocytes from Medaka Embryonic Cells in vitro (2008)
  • Author(s): Shinji Makino, et. al.
  • Organizer: 第72回日本循環器学会
  • Place of Presentation: 福岡
  • Year and Date: 2008-03-29
• [Presentation] Imprintinggene-modified parthenogenic ES cells can be a novel autologous cell source for generating regenerative cardiomyocytes (2008)
  • Author(s): Toshimi Kageyama, Shinji Makino, Keiichi Fukuda
  • Organizer: American Heart Association
  • Place of Presentation: New Orleans(U.S.A.)
• [Book] Cardiovascular Regeneration and Stem Cell Therapy (2007)
  • Author(s): Cardiovascular Regeneration and Stem Cell Therapy
  • Publisher: Blackwell Publishing Ltd