Establishment and clinical application of new assay method for human chymase

• Project/Area Number: 19590841
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Fukuoka University
• Principal Investigator: URATA Hidenori Fukuoka University, 筑紫病院, 教授 (30289524)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 心臓病態学 / レニン・アンジオテンシン系 / キマーゼ / アンジオテンシン変換酵素 / カテプシンG / 心血管病 / 慢性腎障害 / 急性冠動脈症候群 / キマーゼ阻害薬

Research Abstract

まず第一に、特異性の高いキマーゼ阻害薬を使用し、新しいヒトキマーゼ依存性アンジオテンシンII(AII)産生能の測定法を確立した。この方法を用いて、人の末梢血検体を分析し、ヒトキマーゼAII産生能がヒトの循環単核球中に存在し、単球に多く、男性で高値であり、白血球数・CRP、HOMA指数・尿中微量アルブミン/クレアチニン比などと正相関することを見出した。すなわち、生活習慣病の病態で循環単核球中のキマーゼ活性が増加することを明らかにした。

Research Products

• [Journal Article] キマーゼ阻害薬の展望 (2009)
  • Author(s): 浦田秀則
  • Journal Title: 医学のあゆみ レニン・アンジオテンシン系のすべて 228 Pages: 577-582
• [Journal Article] ACE非依存性のアンジオテンシンII産生系 (2009)
  • Author(s): 浦田秀則
  • Journal Title: Heart View 13 Pages: 16-21
• [Journal Article] キマーゼ阻害薬の展望 (2009)
  • Author(s): 浦田秀則
  • Journal Title: 医学のあゆみレニン・アンジオテンシン系のすべて 228 Pages: 577-582
• [Journal Article] レニン・アンジオテンシン系阻害薬の心保護作用を再考する~ACE阻害薬の冠動脈・心筋保護に関する新知見~ (2008)
  • Author(s): 浦田秀則
  • Journal Title: 日系CME(日系メディカル同封別冊) 6
• [Journal Article] Antiarrhythmogenic Effect of Reconstituted High-Density Lipoprotein Against Ischemia/Reperfusion in Rats (2008)
  • Author(s): Imaizmi S, Miura S, Nakamura K, Kiya Y, Uehara Y, Bo Zhang, Matsuo Y, Urata H, Ideishi M, Kerry-Anne Rye, Sata M, Saku K.
  • Journal Title: Journal of the American College of Cardiology 51 Pages: 1604-1612
  • Peer Reviewed
• [Journal Article] Results of provisional stenting with a Sirolimus-eluting stent for bifurcation lesion : Multicenter study in Japan (2008)
  • Author(s): Yanagi D, Shirai K, Takayama Y, Fukada Y, Kuwano T, Ike A, Mori K, Kubota K, Iwata A, Fujimi K, Kawamura A, Nishikawa H, Nathan Millerb, Bo Zhang, Tsuchiya Y, Urata H, Saku K.
  • Journal Title: Journal of Cardiology 51 Pages: 89-94
  • NAID: 10024420942
  • Peer Reviewed
• [Journal Article] Results of provisional stenting with a Sirolimus-eluting stent for bifurcation lesion : Multicenter study in Japan (2008)
  • Author(s): Yanagi D.
  • Journal Title: Journal of Cardiology 51 Pages: 89-94
  • Peer Reviewed
• [Journal Article] Antiarrhythmogenic Effect of Reconstituted High-Density Lipoprotein Against Ischemia/Reperfusion in Rats (2008)
  • Author(s): Imaizumi s.
  • Journal Title: Journal of the American College of Cardiology 51 Pages: 1604-1612
  • Peer Reviewed
• [Journal Article] レニン・アンジオテンシン系阻害薬の心保護作用を再考する〜ACE阻害薬の冠動脈・心筋保護に関する新知見〜 (2008)
  • Author(s): 浦田秀則
  • Journal Title: 日経CME(日経メディカル同封別冊) 6 Pages: 1-7
• [Journal Article] Positive Correlation Between Chymase-Like Angiotensin II -Forming Activity in Mononuclear Cells and Serum Cholesterol Level (2007)
  • Author(s): Murakami K, Uehara Y, Abe S, Inoue Y, Ideishi M, Saku K, Urata H.
  • Journal Title: Journal of Cardiology 50 Pages: 291-298
  • NAID: 10019796038
  • Peer Reviewed
• [Journal Article] Chymase and Matrix Metalloproteinase (2007)
  • Author(s): Urata H.
  • Journal Title: Hypertens Res 30 Pages: 3-4
  • NAID: 10018831222
  • Peer Reviewed
• [Journal Article] Chymase and Matrix Metalloproteinase (2007)
  • Author(s): 浦田 秀則
  • Journal Title: Hypertens Res 30 Pages: 3-4
  • NAID: 10018831222
  • Peer Reviewed
• [Journal Article] Positive Correlation Between Chymase-Like Angiotensin II-Forming Activity in Mononuclear Cells and Serum Cholesterol Level (2007)
  • Author(s): 村上 謹史郎
  • Journal Title: Journal of Cardiology 50 Pages: 291-298
  • Peer Reviewed
• [Presentation] Chymase Dependent Angiotensin II-Forming Activity in the Circulating Mononuclear Leukocyte Increases Post Acute Myocardial Infarction (2009)
  • Author(s): Okamura K, Inoue Y, Uehara Y, Maruyama S, Sumi S, Furuyama S, Kodama S, Andoh C, Niimura H, Tohjoh H, Miyoshi K, Miura S, Yamanouchi Y, Saku K, Urata H.
  • Organizer: 第73回日本循環器学会総会・学術集会
  • Year and Date: 2009-03-21
• [Presentation] Chymase Dependent Angiotensin II-Forming Activity in the Circulating Mononuclear Leukocyte Increases Post Acute Myocardial Infarction (2009)
  • Author(s): Keisuke Okamura
  • Organizer: 第73回日本循環器学会総会・学術集会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-03-21
• [Presentation] In vitro profile of a novel chymase inhibitor and the improvement of survival in hamsters with myocardial infarction (2008)
  • Author(s): Takahashi Y, Hase N, Muroga Y, Matsuura J, Machida T, Kobayashi T, Yamanaka Y, Kasahara Y, Urata H.
  • Organizer: 第12回日本心不全学会学術集会
  • Year and Date: 2008-10-18
• [Presentation] In vitro profile of a novel chymase inhibitor and the improvement of survival in hamsters with myocardial infarction (2008)
  • Author(s): Y Takahashi
  • Organizer: 第12回日本心不全学会学術集会
  • Place of Presentation: ホテルパシフィッフ東京
  • Year and Date: 2008-10-18
• [Presentation] Chymase activity in the circulating mononuclear increases in chronic kidney disease patients (2008)
  • Author(s): Okumura K
  • Organizer: 第31回日本高血圧学会総会
  • Year and Date: 2008-10-10
• [Presentation] Chymase activity in the circulating mononuclear increases in chronic kidney disease patients (2008)
  • Author(s): Keisuke Okamura
  • Organizer: 第31回日本高血圧学会総会
  • Place of Presentation: ロイトン札幌
  • Year and Date: 2008-10-10
• [Presentation] Chymase activity in the circulating mononuclear leukocyte increases in chronic kidney disease patients (2008)
  • Author(s): Okamura K
  • Organizer: 第12回Molecular Cardiovascular Conference
  • Year and Date: 2008-09-06
• [Presentation] Chymase activity in the circulating mononuclear leukocyte increases in chronic kidney disease patients (2008)
  • Author(s): Keisuke Okamura
  • Organizer: 第12回Molecular Cardiovascular Conference
  • Place of Presentation: キロロホテルピアノ
  • Year and Date: 2008-09-06
• [Presentation] High chymase activity in the circulating mononuclear leukocyte is related to high c-reactive protein and insulin levels (2008)
  • Author(s): Okamura k, Inoue Y, Uehara Y, Sumi S, Kudo K, Kodama S, Ando C, Mihara H, Niimura H, Miyoshi K, Tsuchiya Y, Miura S, Yamanouchi Y, Saku K, Urata H.
  • Organizer: ISH2008
  • Year and Date: 2008-06-16
• [Presentation] Enhancement of angiotensin II forming activity in mononuclear cells by free fatty acids (2008)
  • Author(s): Azekoshi Y, Yamakawa K, Yasu T, Abe S, Tagomori Y, Urata H, Ueda S.
  • Organizer: ISH2008
  • Year and Date: 2008-06-16
• [Presentation] The role of chymase in angiotensin II formation and progression of fibrosis in obstructed kidneys (2008)
  • Author(s): Y. -Y. Fan, Kohno M, Fujisawa Y, Kimura S, Matsuura J, Hase N, Hitomi H, Urata H.
  • Organizer: ISH2008
  • Year and Date: 2008-06-16
• [Presentation] High chymase activity in the circulating mononuclear leukocyte is related to high c-reactive protein and insulin levels (2008)
  • Author(s): Okamura K, Inoue Y, Uehara Y, Abe S, Sumi S, Kudo K, Kodama S, Ando C, Mihara H, Niimura H, Miyoshi K, Tsuchiya Y, Miura S, Yamanouchi Y, Saku K, Urata H.
  • Organizer: 第72回日本循環器学会総会・学術集会
  • Year and Date: 2008-03-29
• [Presentation] High chymase activity in the circulating mononuclear leukocyte is related to high c-reactive pxotein and insulin levels (2008)
  • Author(s): 岡村 圭祐
  • Organizer: 第72回日本循環器学会総会・学術集会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-03-29
• [Presentation] ヒト信三キマーゼの同定 (2007)
  • Author(s): 浦田秀則
  • Organizer: 第30回日本高血圧学会総会
  • Year and Date: 2007-10-26
• [Presentation] ヒト心臓キマーゼの同定 (2007)
  • Author(s): 浦田 秀則
  • Organizer: 第30回日本高血圧学会総会
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2007-10-26
• [Presentation] Chymase Inhibitor in the Treatment of Heart Failure (2007)
  • Author(s): Urata H
  • Organizer: 第11回日本心不全学会学術集会
  • Year and Date: 2007-09-10
• [Presentation] Chymase Inhibitor in the Treatment of Heart Failure (2007)
  • Author(s): 浦田 秀則
  • Organizer: 第11回日本心不全学会学術集会
  • Place of Presentation: ヒルトン東京ベイ
  • Year and Date: 2007-09-10
• [Presentation] Beneficial effects of ACEI on Coronary Heart Disease (2007)
  • Author(s): Urata H
  • Organizer: ヨーロッパ心臓病学会
  • Year and Date: 2007-09-03
• [Presentation] Beneficial effects of ACEI on Coronary Heart Disease (2007)
  • Author(s): Urata H.
  • Organizer: ヨーロッパ心臓病学会2007
  • Place of Presentation: MESSE WIEN
  • Year and Date: 2007-09-03