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Mechanisms and physiology of Ca^<2+> influx by spatial regulation ofcaveolae in endothelial cells

Research Project

Project/Area Number 19590852
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionThe University of Tokyo

Principal Investigator

ISSHIKI Masashi  The University of Tokyo, 医学部・附属病院, 助教 (70302734)

Co-Investigator(Renkei-kenkyūsha) TOSHIRO Fujita  東京大学, 医学部・附属病院, 教授 (10114125)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsシグナル伝達 / 高血圧 / イメージング / 内皮機能 / 細胞生物学 / 循環器・高血圧 / マイクロドメイン
Research Abstract

培養血管内皮細胞において、急性期のアルドステロン前処置はATPによるeNOSリン酸化亢進を介してNO産生を増強する。また、MR ブロッカーエプレレノンはSREBP1の活性化によるcaveolin-1発現の低下、caveolin1非結合型フリーeNOS増加、eNOSリン酸化増強を介してNO産生をきたす。この現象はおそらくMR 非依存的であり、メタボリックシンドロームモデル動物であるSHRcpでも同様の現象が観察された。エプレレノンはこの局所膜マイクロドメインにおいてこれまで知られなかった新しい機序で内皮機能を改善する効果を発揮する可能性が見いだされた。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (12 results)

All 2009 2008 2007

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (9 results)

  • [Journal Article] Aldosterone enhances ligand-stimulatednitric xoide production in endothelial cells2008

    • Author(s)
      Mutoh A, Isshiki M, Fujita T.
    • Journal Title

      Hypertens Res 31

      Pages: 1811-20

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aldosterone enhances lig and-stimulated Nitric Oxide Production in Endothelial cells.2008

    • Author(s)
      Mutoh A.,Isshiki M., Fujita T.
    • Journal Title

      Hypertens Res 31

      Pages: 1811-1820

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Up-Regulation of JAM-1 in AR42J Cells Treated with Activin A and Betacellulin and the Diabet ic Regenerating Islets.2008

    • Author(s)
      Yoshikumi Y. 他
    • Journal Title

      Endocr J 55

      Pages: 757-765

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Eplerenone down-regulaes caveolin-1and improves endothelial function bymechanisms independent of Mrantagonism2009

    • Author(s)
      Muto-A, Isshiki M, Nishimoto-M, Fujita T.
    • Organizer
      The 2^<nd>International Aldosterone Forum in Japan(Award winners' oral presentation)
    • Place of Presentation
      東京
    • Year and Date
      2009-05-17
    • Related Report
      2008 Final Research Report
  • [Presentation] 低用量エプレレノンはMR 非依存的に内皮機能を改善する2009

    • Author(s)
      武藤明子, 一色政志, 西本光宏, 藤田敏郎
    • Organizer
      日本内分泌学会
    • Place of Presentation
      前橋
    • Year and Date
      2009-04-24
    • Related Report
      2008 Final Research Report
  • [Presentation] Low-dose eplerenone down-regulatescaveolin-1 and improves endothelialfunction by mechanisms independent of Mrantagonism2008

    • Author(s)
      Mutoh-A, Isshiki M. and Fujita T.
    • Organizer
      AHA Scientific Sessions 2008 (oralpresentation)
    • Place of Presentation
      ニューオーリンズ、米国
    • Year and Date
      2008-11-10
    • Related Report
      2008 Final Research Report
  • [Presentation] Low-dose eplerenone down-regulates caveolin-1 and improves endothelial function by mechanisms independent of MR antagonism2008

    • Author(s)
      Mutoh Akiko
    • Organizer
      AHA Scientific Meeting 2008
    • Place of Presentation
      ニューオーリンズ、米国
    • Year and Date
      2008-11-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] エプレレノンによるメタボリックシンドロームラットの血管内皮機能改善とその機序2008

    • Author(s)
      武藤明子、一色政志、西本光宏、藤田敏郎
    • Organizer
      日本高血圧学会
    • Place of Presentation
      札幌
    • Year and Date
      2008-10-10
    • Related Report
      2008 Final Research Report
  • [Presentation] エプレレノンによる肥満高血圧ラットの血管内皮機能改善とその機序2008

    • Author(s)
      武藤明子、一色政志、西本光宏、藤田敏郎
    • Organizer
      日本内分泌学会
    • Place of Presentation
      青森
    • Year and Date
      2008-05-17
    • Related Report
      2008 Final Research Report
  • [Presentation] LOX-1 upregulation shifts endocytosisof cholera toxin from caveolae-mediated tononcaveolae-mediated pathway inendothelial cells2007

    • Author(s)
      Mutoh-A, Isshiki and M. Fujita T.
    • Organizer
      AHA Scientific Sessions 2007(poster presentation)
    • Place of Presentation
      オーランド、米国
    • Year and Date
      2007-11-08
    • Related Report
      2008 Final Research Report
  • [Presentation] アルドステロンはリガンド刺激によるeNOSリン酸化の増強を介して血管内皮NO 産生を亢進する2007

    • Author(s)
      武藤明子, 高良洋平, 一色政志, 藤田敏郎
    • Organizer
      日本高血圧学会
    • Place of Presentation
      那覇
    • Year and Date
      2007-10-26
    • Related Report
      2008 Final Research Report
  • [Presentation] 血管内皮細胞の局所Ca^<2+>シグナルを構成する膜直下サブドメイン2007

    • Author(s)
      一色 政志
    • Organizer
      日本内分泌学会
    • Place of Presentation
      東京
    • Year and Date
      2007-06-15
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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