Role of DNA double strand breaks and repair in atherosclerosis formation and progression.

• Project/Area Number: 19590865
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Hiroshima University
• Principal Investigator: ISHIDA Mari Hiroshima University, 大学院・医歯薬学総合研究科, 講師 (30359898)
• Co-Investigator(Kenkyū-buntansha): ISHIDA Takafumi 広島大学, 大学院・医歯薬学総合研究科, 講師 (40346482)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 血管病態学 / 動脈硬化 / DNA損傷 / 二重鎖切断 / DNA損傷修復 / 循環器 / ゲノム障害 / 老化 / 血管

Research Abstract

本研究により、ヒト動脈硬化巣にはDNA損傷、とくに二重鎖切断が存在し、これを修復するためにゲノム損傷修復酵素の活性化が認められること、さらに、動脈硬化マウスのゲノム修復機構を阻害すると動脈硬化が増悪することが示された。本研究結果から、動脈硬化の成因あるいはその進展にDNA損傷とそれに対する修復機構の異常が大きく関与していることが明らかとなった。

Research Products

• [Journal Article] Angiotensin II-induced Osteopontin Expression in Vascular Smooth Muscle Cells Involves G_<q/11>, Ras, ERK, Src and Ets-1. (2008)
  • Author(s): Shimote-Abe K, Hidekatsu Nakashima, Mariko Sawano, Mari Ishida, New New Soe, Masao Yoshizumi, Takafumi Ishida
  • Journal Title: Hypertens. Res. 31 Pages: 987-998
  • NAID: 10021935864
  • Peer Reviewed
• [Journal Article] Angiotensin II-induced Osteopontin Expression in Vascular Smooth Muscle Cells Involves G_<q/11>, Ras, ERK, Src and Ets-1 (2008)
  • Author(s): Abe K, et.al
  • Journal Title: Hypertension Research 31 Pages: 987-998
  • Peer Reviewed
• [Journal Article] Angiotensin II-induced Osteopontin Expression in Vascular Smooth Muscle Cells Involves G_<q/11>, Ras, ERK, Src and Ets-1 (2008)
  • Author(s): Keiko Abe
  • Journal Title: Hypertension Reasearch (In press)
  • Peer Reviewed
• [Presentation] 動脈硬化の成因としてのゲノム損傷・修復 (2010)
  • Author(s): 石田万里
  • Organizer: 第93回日本循環器学会中国地方会
  • Place of Presentation: 広島
  • Year and Date: 2010-11-29
• [Presentation] Role of Src and Cas in dorsal ruffling, lamellipodia and migration in vascular smooth muscle cells. (2009)
  • Author(s): Soe NN, Ishida T, Ishida M, Abe K, Nishioka K, Okada T, Shokawa T, Nakano Y, Teragawa H, Tadehara F, Yamamoto H, Kihara Y, Yoshizumi M
  • Organizer: 第73回日本循環器学会総会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-03-20
• [Presentation] Role of Src and Cas in dorsal ruffling, lamellipodia and migration in vascular smooth muscle cells (2009)
  • Author(s): Soe NN
  • Organizer: 第73回日本循環器学会総会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-03-20
• [Presentation] 動脈硬化の成因としてのゲノム損傷・修復 (2008)
  • Author(s): 石田万里, 石田隆史, スー・ヌエヌエ, 澤野真理子, 山本秀也, 蓼原太, 荘川知己, 岡田武規, 寺川宏樹, 中野由紀子, 西岡健司, 木原康樹, 吉栖正雄
  • Organizer: 第93回日本循環器学会中国地方会
  • Place of Presentation: 広島・呉
  • Year and Date: 2008-11-29
• [Presentation] DNA Double-Strand Breaks: A possible mechanisms for Atheroscleroticformation and progression. (2008)
  • Author(s): 石田万里
  • Organizer: 第5回国際シンポジウム(21世紀COEプログラム)
  • Place of Presentation: 広島
  • Year and Date: 2008-01-24
• [Presentation] Yasuki Kihara and Takafumi Ishida The Role of DNA Double Strand Breaks in Atherosclerosis and Vascular Smooth Muscle Cell Apoptosis. (2008)
  • Author(s): Mari Ishida, Noriko Kuwaba, Mariko Sawano, Mika Nakashima, Keiko Abe, Nwe Nwe Soe, Masao Yoshizumi
  • Organizer: 第12回Molecular Cardiovascular Conference
  • Place of Presentation: 北海道
• [Presentation] c-Src and Its Substrates Play a Pivotal Role in Migration of Vascular Smooth Muscle Cells. (2008)
  • Author(s): Soe NN, 石田隆史, 石田万里, 三保成正, 澤野真理子, 阿部恵子, 茶山一彰, 吉栖正生
  • Organizer: 日本動脈硬化学会学術集会
  • Place of Presentation: 筑波
• [Presentation] c-Src and Its Substrates Play a Pivotal Role in Migration of Vascular Smooth Muscle Cells (2008)
  • Author(s): Soe NN, Ishida M, Miho N, Sawano M, Abe K, Chayama K, Yoshizumi M, Ishida T
  • Organizer: AHA Arterioscelrosis, Thrombosis and Vascular Biology Annual Conference
  • Place of Presentation: Atlanta, USA
• [Presentation] c-Src and Its Substrates Play a Pivotal Role in Migration of Vascular Smooth Muscle Cells (2008)
  • Author(s): Soe NN
  • Organizer: AHA Arterioscelrosis, Thrombosis and Vascular Biology Annual Conference 2008
  • Place of Presentation: Atlanta, USA
• [Presentation] c-Src and Its Substrates Play a Pivotal Role in Migration of Vascular Smooth Muscle Cells (2008)
  • Author(s): Soe NN
  • Organizer: 日本動脈硬化学会学術集会
  • Place of Presentation: 筑波
• [Presentation] The Role of DNA Double Strand Breaks in Atherosclerosis and Vascular Smooth Muscle Cell Apoptosis (2008)
  • Author(s): 石田万里
  • Organizer: 第12回 Molecular Cardiovascular Conference
  • Place of Presentation: 北海道
• [Presentation] Angiotensin II-induced osteopontin expression in vascular smooth muscle cells involves Gq/11, Ras, ERK, Src and Ets-1. (2007)
  • Author(s): 阿部恵子
  • Organizer: American Heart Association Scientific Sessions 2007
  • Place of Presentation: Orlando
  • Year and Date: 2007-11-16
• [Presentation] Angiotensin IIによるOsteopontinの発現にはGq/11, Src, Ras, ERKが関与する (2007)
  • Author(s): 外間梨沙
  • Organizer: 第30回日本高血圧学会
  • Place of Presentation: 沖縄
  • Year and Date: 2007-10-25
• [Presentation] Nefedipineの血管平滑筋細胞遊走・肥大およびその細胞内シグナル伝達機構におよぼす作用 (2007)
  • Author(s): 澤野真理子
  • Organizer: 第30回日本高血圧学会
  • Place of Presentation: 沖縄
  • Year and Date: 2007-10-25