Application of RNA interference for therapeutic approach to refractory respiratory diseases
| Project/Area Number |
19590896
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Hiroshima University |
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Principal Investigator |
HATTORI Noboru Hiroshima University, 大学院・医歯薬学総合研究科, 准教授 (00283169)
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| Co-Investigator(Kenkyū-buntansha) |
横山 彰仁 高知大学, 医学部, 教授 (30191513)
石川 暢久 広島大学, 大学院・医歯薬学総合研究科, 助教 (90423368)
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| Co-Investigator(Renkei-kenkyūsha) |
YOKOYAMA Akihito 高知大学, 医学部, 教授 (30191513)
ISHIKAWA Nobuhisa 広島大学, 大学院・医歯薬学総合研究科, 助教 (90423368)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | RNA干渉 / 肺線維症 / 肺癌 / 気管支喘息 / GFP |
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| Research Abstract |
In the present study, we tried to determine whether intratracheal administration of siRNA against green fluorescence protein (GFP) could inhibit gene expression of GFP in the lungs of GFP-transgenic mouse or in the lung tumor which was generated by the intrathoracic inoculation of Lewis lung adenocarcinoma (LLC) cells over-expressing GFP (GFP-LLC). To detect fluorescence from GFP in the lung, Biozero fluorescence microscope (Keyence, Osaka, Japan) was used. The strong fluorescence signals were observed both in the lung of GFP transgenic mice and in the lung tumor consisting of GFP-LLC cells. However, intratracheal administration of siRNA against GFP did not inhibit the degree of florescence signal. The amount of siRNA used in the study might be insufficient.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Usefulness of monitoring the circulating Krebs von den Lungen-6 levels to predict the clinical outcome of patients with advanced nonsmall cell lung cancer treated with epidermal growth factor receptor tyrosine kinase inhibitors.2008
Author(s)
Ishikawa N, Hattori N, Yokoyama A, Tanaka S, Nishino R, Yoshioka K, Ohshimo S, Fujitaka K, Ohnishi H, Hamada H, Arihiro K, Kohno N.
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Journal Title
Int J Cancer 122
Pages: 2612-2620
Related Report
Peer Reviewed
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[Journal Article] Cancer-testis antigen lymphocyte antigen 6 complex locus K is a serologic biomarker and a therapeutic target for lung and esophageal carcinomas.2007
Author(s)
Ishikawa N, Takano A, Yasui W, Inai K, Nishimura H, Ito H, Miyagi Y, Nakayama H, Fujita M, Hosokawa M, Tsuchiya E, Kohno N, Nakamura Y, Daigo Y.
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Journal Title
Cancer Res 1567
Pages: 11601-11611
Related Report
Peer Reviewed
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