Significance of Wnt signaling in pleural mesothelioma

• Project/Area Number: 19590910
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Saitama Medical University
• Principal Investigator: UEMATSU Kazutsugu Saitama Medical University, 医学部, 准教授 (10297880)
• Co-Investigator(Kenkyū-buntansha): 森山 岳 埼玉医科大学, 医学部, 助教 (30364798)
• Co-Investigator(Renkei-kenkyūsha): MORIYAMA Gaku 埼玉医科大学, 医学部, 助教 (30364798)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 胸膜中皮腫 / WNTシグナル / Dishevelled(Dvl) / 中皮腫 / Wntシグナル / Dvl / Dv1

Research Abstract

To clarify the effect of blocking Wnt signaling in mesothelioma, we down-regulated the expression of dishevelled (Dvl), an intermediator of Wnt signaling, by a small interfering RNA (siRNA). Mesothelioma cell lines were transfected with siRNA of Dvl, and cell growth was examined. The synergistic effect on cell growth of Dvl siRNA and cisplatin in combination was evaluated. Dvl siRNA down-regulated the expression of Dvl in mesothelioma cells, which caused suppression of cell growth. Combination of Dvl siRNA and cisplatin suppressed cell growth synergistically. Our data suggest that inhibition of Wnt signaling leads to significant anti-tumor effects.

Research Products

• [Journal Article] Frequency of and variables associated with the EGFR mutation and its subtypes. (2010)
  • Author(s): Tanaka T, Matsuoka M, Sutani A, Gemma A, Maemondo M, Inoue A, Okinaga S, Nagashima M, Oizumi S, Uematsu K, Nagai Y, Moriyama G, Miyazawa H, Ikebuchi K, Morita S, Kobayashi K, Hagiwara K.
  • Journal Title: International Journal of Cancer 126 Pages: 651-655
  • Peer Reviewed
• [Journal Article] Frequency of and variables associated with the EGFR mutation and its subtypes (2010)
  • Author(s): Tanaka T, Matsuoka M, Sutani A, Gemma A, Maemondo M, Inoue A, Okinaga S, Nagashima M, Oizumi S, Uematsu K, Nagai Y, Moriyama G, Miyazawa H, Ikebuchi K, Morita S, Kobayashi K, Hagiwara K.
  • Journal Title: International Journal of Cancer 126 Pages: 651-655
  • Peer Reviewed
• [Journal Article] Reduced transcription of the Smad4 gene during pulmonary carcinogenesis in idiopathic pulmonary fibrosis. (2009)
  • Author(s): Takenaka T, Gemma A, Yoshimura A, Hosoya Y, Nara M, Hosomi Y, Okano T, Kunugi S, Koizumi K, Fukuda Y, Uematsu K, Shimizu K, Kudoh S.
  • Journal Title: Molecular Medicine Reports Vol.2 Pages: 73-80
  • Peer Reviewed
• [Journal Article] Reduced transcription of the Smad4 gene during pulmonary carcinogenesis in idiopathic pulmonary fibrosis (2009)
  • Author(s): Takenaka T, Gemma A Yoshimura A, Hosoya Y, Nara M, Hosomi Y, Okano T, Kunugi S, Koizumi K, Fukuda Y, Uematsu K, Shimizu K, Kudoh S.
  • Journal Title: Molecular Medicine Reports 2 Pages: 73-80
  • Peer Reviewed
• [Journal Article] Risk factors for a second episode of hemoptysis. (2009)
  • Author(s): Seki N, Shiozaki G, Ota M. 0ta S, Seki R, Seto T, Uemateu K, Eeuchi K
  • Journal Title: Clinical Medicine : Circulatory, Respiratoryand Pulmonarv Medicine 3 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Tegafur-uracil plus gemcitabine combination chemotherapy in patients with advanced non-small cell lung cancer previously treated with platinum. (2008)
  • Author(s): Seto T, Yamanaka T, Nakano M, Ota M, Maruyama R, Okamoto T, Wataya H, Uematsu K, Seki N, Eguchi K, Semba H, Ichinose Y.
  • Journal Title: Journal of Thoracic Oncology Vol.3 Pages: 637-642
  • Peer Reviewed
• [Journal Article] Tegafur-uracil plus gemcitabine combination chemotherapy in patients with advanced non-small cell lung cancer previously treated with platinum. (2008)
  • Author(s): Seto T, Yamanaka T, Nakano M, Ota M, Maruyama R, Okamoto T, Wataya H, Uematsu K, Seki N, Eguchi K, Semba H, Iehinose Y.
  • Journal Title: Journal of Thoracic Oncology 3 Pages: 637-642
  • Peer Reviewed
• [Journal Article] Inhibitory effect of budesonide alone and in combination with formoterol on IL-5 and RANTES production from mononuclear cells. (2008)
  • Author(s): Soma T, Takaku Y, Kobayashi T, Hagiwara K, Kanazawa M, Uematsu K, Naeata M
  • Journal Title: International Archives of Allergy and Immunology 146 Suppl Pages: 22-27
  • Peer Reviewed
• [Journal Article] Targeting the Wnt Signaling Pathway with Dishevelled and Cisplatin Synergistically Suppresses Mesothelioma Cell Growth. (2007)
  • Author(s): Uematsu K, Seki N, Seto T, Isoe C, Tsukamoto H, Mikami I, You L, He B, Xu Z, Jablons DM, Eguchi K.
  • Journal Title: Anticancer Research Vol.27 Pages: 4239-4242
• [Journal Article] 中皮腫発症の分子機構. (2007)
  • Author(s): 植松和嗣
  • Journal Title: 呼吸器科 12巻 Pages: 296-303
• [Journal Article] Targeting the Wnt Signaling Pathway with Dishevelled and Cisplatin Synergistically Suppresses Mesothelioma Cell Growth (2007)
  • Author(s): Uematsu K., Seki N., Seto T., Isoe C., Tsukamoto H., Mikami I., You L., He B., Xu Z., Jablons D.M., Eguchi K.
  • Journal Title: Anticancer Research 27 Pages: 4239-4242
  • Peer Reviewed
• [Journal Article] 中皮腫発症の分子機構 (2007)
  • Author(s): 植松 和嗣
  • Journal Title: 呼吸器科 12 Pages: 296-303
• [Presentation] 当院における若年者肺癌臨床的検討. (2008)
  • Author(s): 森山岳, 山本和男, 山名一平, 大岩健満, 杣知行, 植松和嗣, 小林国彦, 弦間昭彦, 工藤翔二
  • Organizer: 第48回日本呼吸器学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2008-06-15
• [Presentation] 当院における若年者肺癌臨床的検討 (2008)
  • Author(s): 森山岳, 山本和男, 山名一平, 大岩健満, 杣知行, 植松和嗣, 小林国彦, 弦間昭彦, 工藤翔二
  • Organizer: 第48回日本呼吸器学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2008-06-15
• [Presentation] 胸膜中皮腫細胞におけるWntシグナル伝達体Dishevelled (Dvl)の発現抑制によるタンパク発現の変化の検討. (2007)
  • Author(s): 植松和嗣、関順彦、大岩健満、北村和広、森山岳、山本和男、杣知行、篠田欣也、江口研二
  • Organizer: 第47回日本呼吸器学会総会
  • Place of Presentation: 東京
  • Year and Date: 2007-05-12
• [Presentation] 胸膜中皮腫細胞におけるWntシグナル伝達体Dishevelled(Dvl)の発現抑制によるタンパク発現の変化の検討 (2007)
  • Author(s): 植松 和嗣、関 順彦、大岩 健満、北村 和広、森山 岳、山本 和男、杣 知行、篠田 欣也、江口 研二
  • Organizer: 第47回日本呼吸器学会総会
  • Place of Presentation: 東京
  • Year and Date: 2007-05-12