| Project/Area Number |
19590913
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
|
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| Research Institution | Keio University |
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Principal Investigator |
TASAKA Sadatomo Keio University, 医学部, 助教 (70276244)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMASAWA Wakako 慶應義塾大学, 医学部, 助教 (50317137)
HASEGAWA Naoki 慶應義塾大学, 医学部, 講師 (20198724)
SAITO Fumitake 慶應義塾大学, 医学部, 助教 (30338040)
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| Project Period (FY) |
2007 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
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| Keywords | 低酸素反応性因子 / 急性呼吸不全 |
|---|
| Research Abstract |
We evaluated the role of hypoxia-inducible factor-1 (HIF-1), which mediates various changes during hypoxia, in the pathogenesis of acute lung inflammation. Inflammatory stimulus upregulated the gene expression of HIF-1 in cultured endothelial and epithelial cells. In addition, upregulation of HIF-1 was observed in the airway epithelial lining fluid in patients with acute respiratory failure. Next, we tried to inhibit the expression of HIF-1 in cultured cells using RNA interference. Although, in some studies, the HIF-1 expression was inhibited almost completely, the efficacy of inhibition was not stable. In addition, it was examined whether intratracheal administration of siRNA selectively inhibits the HIF-1 expression in the respiratory system. Since the results were widely variable, any robust conclusion could not be obtained concerning the role of HIF-1.
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