Role of hypoxia-inducible factor in acute respiratory failure

• Project/Area Number: 19590913
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Keio University
• Principal Investigator: TASAKA Sadatomo Keio University, 医学部, 助教 (70276244)
• Co-Investigator(Kenkyū-buntansha): YAMASAWA Wakako 慶應義塾大学, 医学部, 助教 (50317137) ; HASEGAWA Naoki 慶應義塾大学, 医学部, 講師 (20198724) ; SAITO Fumitake 慶應義塾大学, 医学部, 助教 (30338040)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 低酸素反応性因子 / 急性呼吸不全

Research Abstract

We evaluated the role of hypoxia-inducible factor-1 (HIF-1), which mediates various changes during hypoxia, in the pathogenesis of acute lung inflammation. Inflammatory stimulus upregulated the gene expression of HIF-1 in cultured endothelial and epithelial cells. In addition, upregulation of HIF-1 was observed in the airway epithelial lining fluid in patients with acute respiratory failure. Next, we tried to inhibit the expression of HIF-1 in cultured cells using RNA interference. Although, in some studies, the HIF-1 expression was inhibited almost completely, the efficacy of inhibition was not stable. In addition, it was examined whether intratracheal administration of siRNA selectively inhibits the HIF-1 expression in the respiratory system. Since the results were widely variable, any robust conclusion could not be obtained concerning the role of HIF-1.

Research Products

• [Remarks]
  • URL: http://web.sc.itc.keio.ac.jp/medicine/pulmonary/index.html