Identification of chemosensitivity predictive factors in lung cancer cells and chemosensitivity prediction by image

• Project/Area Number: 19590919
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Nippon Medical School
• Principal Investigator: GENMA Akihiko Nippon Medical School, 大学院・医学研究科, 教授 (20234651)
• Co-Investigator(Kenkyū-buntansha): KUDOH Shoji 日本医科大学, 医学部, 名誉教授 (40256912) ; SEIKE Masahiro 日本医科大学, 医学部, 講師 (30366687) ; OKANO Tetsuya 日本医科大学, 医学部, 講師 (00339376)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 肺癌 / 薬剤感受性 / プロテオーム / イメージング / 抗体アレイ / 2D-DIGE / EGFR / VEGFR / 薬物療法 / 分子標的薬 / バイオイメージング / "D-DIGE

Research Abstract

肺癌における化学療法を合理的に選択するシステムを確立するため、肺癌細胞株の網羅的発現解析と感受性解析を行い、バイオイメージング可能な感受性関連因子の選択を行った。その中で、EGFRとVEGFRの阻害剤に対して、標的そのものが妥当なイメージング対象と考えられ、培養株およびマウスモデルで、同様のイメージング結果が定量的に得られた。これらのイメージングの情報により、標的分子を有する腫瘍量をリアルタイムに定量的に把握できることから、分子標的薬の治療適応により直接的な情報が得られると考えられた。

Research Products

• [Journal Article] Exacerbation of idiopathic interstitial pneumonias associated with lung cancer therapy. (2009)
  • Author(s): Minegishi Y, Takenaka K, Mizutani H, Sudoh J, Noro R, Okano T, Azuma A, Yoshimura A, Ando M, Tsuboi E, Kudoh S, Gemma A.
  • Journal Title: Intern Med. 48(9) Pages: 665-72
  • NAID: 130000122480
  • Peer Reviewed
• [Journal Article] North East Japan Gefitinib Study Group. First-line gefitinib for patients with advanced non-small-cell lung cancer harboring epidermal growth factor receptor mutations without indication for chemotherapy. (2009)
  • Author(s): Inoue A, Kobayashi K, Usui K, Maemondo M, Okinaga S, Mikami I, Ando M, Yamazaki K, Saijo Y, Gemma A, Miyazawa H, Tanaka T, Ikebuchi K, Nukiwa T, Morita S, Hagiwara K
  • Journal Title: J Clin Oncol. 27(9) Pages: 1394-400
  • Peer Reviewed
• [Journal Article] Clinicopathological analysis of primary lung carcinoma with heterotopic ossification. (2009)
  • Author(s): Kuribayashi H, Tsuta K, Mizutani E, Maeshima AM, Yoshida Y, Gemma A, Kudoh S, Asamura H, Matsuno Y
  • Journal Title: Lung Cancer. 64(2) Pages: 160-5
  • Peer Reviewed
• [Journal Article] First-line gefitinib for patients with advanced non-small-cell lung cancer harboring epidermal growth factor receptor mutations without indication for cbemotherapy. (2009)
  • Author(s): Inoue A
  • Journal Title: J Clin Oncol 27(9) Pages: 1394-400
  • Peer Reviewed
• [Journal Article] Predominant infiltration of macrophages and CD8(+)T Cells in cancer nests is a significant predictor of survival in stage IV nonsmall cell lung cancer. (2008)
  • Author(s): Kawai O, Ishii G, Kubota K, Murata Y, Naito Y, Mizuno T, Aokage K, Saijo N, Nishiwaki Y, Gemma A, Kudoh S, Ochiai A.
  • Journal Title: Cancer 113(6) Pages: 1387-95
  • Peer Reviewed
• [Journal Article] Antitumor activity of histone deacetylase inhibitors in non-small cell lung cancer cells : development of a molecular predictive model. (2008)
  • Author(s): Miyanaga A, Gemma A, Noro R, Kataoka K, Matsuda K, Nara M, Okano T, Seike M, Yoshimura A, Kawakami A, Uesaka H, Nakae H, Kudoh S.
  • Journal Title: Mol Cancer Ther. Pages: 1923-30
  • Peer Reviewed
• [Journal Article] Antitumor activity of bistone deacetylase inhibitors in non-small cell lung cancer cells : development of a molecular predictive model. (2008)
  • Author(s): Miyanaga A
  • Journal Title: Mol Cancer Ther. 7(7) Pages: 1923-30
  • Peer Reviewed
• [Journal Article] E-cadherin expression and epidermal growth factor receptor mutation status predict outcome in non-small ceil lung cancer patients treated with gefitinib. (2008)
  • Author(s): Miyanaga A
  • Journal Title: Oncol Rep 19(2) Pages: 377-83
  • Peer Reviewed
• [Journal Article] Gemcitabine resistance in a highly metastatic subpopulation of a pulmonary adenocarcinoma cell line resistant to gefitinib. (2007)
  • Author(s): Takemura A, Gemma A, Shibuya M, Matsuda K, Okano T, Nara M, Noro R, Minegishi Y, Yoshimura A, Kudoh S.
  • Journal Title: Int J Oncol. 31(6) Pages: 1325-32
  • Peer Reviewed
• [Journal Article] PTEN inactivation in lung cancercells and the effect of its recovery on treatment with epidermal growth factor receptor tyrosine kinase inhibitors. (2007)
  • Author(s): Noro R, Gemma A, Miyanaga A, Kosaihira S, Minegishi Y, Nara M, Kokubo Y, Seike M, Kataoka K, Matsuda K, Okano T, Yoshimura A, Kudoh S.
  • Journal Title: Int J Oncol. 31(5) Pages: 1157-63
  • Peer Reviewed
• [Journal Article] Use of a cytokine gene expression signature in lung adenocarcinoma and the surrounding tissue as a prognostic classifier. (2007)
  • Author(s): Seike M, Yanaihara N, Bowman ED, Zanetti KA, Budhu A, Kumamoto K, Mechanic LE, Matsumoto S, Yokota J, Shibata T, Sugimura H, Gemma A, Kudoh S, Wang XW, Harris CC.
  • Journal Title: J Natl Cancer Inst 99(16) Pages: 1257-69
  • Peer Reviewed
• [Journal Article] In vitro simulation study of individualized chemotherapy in lung cancer. (2007)
  • Author(s): Li C, Gemma A, Minegishi Y, Matsuda K, Seike Y, Noro R, Shionoya A, Kawakami A, Ogawa N, Kudoh S
  • Journal Title: J Nippon Med Sch. 74(3) Pages: 217-22
  • Peer Reviewed
• [Journal Article] Gemcitabine resistance in a highly metastatic subpopulation of a pulmonary adenocarcinoma cell line resistant to gefitinib. (2007)
  • Author(s): Takemura A,
  • Journal Title: Int J Oncol. 31(6) Pages: 1325-1332
  • Peer Reviewed
• [Journal Article] PTEN inactivation in lung cancer cells and the effect of its recovery on treatment with epidermal growth factor receptor tyrosine kinase inhibitors. (2007)
  • Author(s): Noro R,
  • Journal Title: Int J Oncol. 31(5) Pages: 1157-1163
  • Peer Reviewed
• [Journal Article] In vitro simulation study of individualized chemotherapy in lung cancer. (2007)
  • Author(s): Li C,
  • Journal Title: J Nippon Med Sch. 74(3) Pages: 217-222
  • Peer Reviewed
• [Presentation] Gefitinib耐性肺癌細胞株におけるZD6474の抗腫瘍効果 (2008)
  • Author(s): 奈良道哉
  • Organizer: 日本呼吸器学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-06-17
• [Presentation] 抗癌剤のリスクとベネフィットーイレッサを中心に (2007)
  • Author(s): 弦間昭彦
  • Organizer: 特別シンポジウム「呼吸器領域におけるリスクアセスメントとリスクコミュニケーション」第47回日本呼吸器学会
• [Book] 抗がん剤d. 間質性肺炎医薬品副作用ハンドブック(高橋隆一編集) (2008)
  • Author(s): 弦間昭彦
  • Publisher: 日本臨床社(東京)
• [Book] 第12章癌緊急対応と支持療法4抗癌剤による間質性肺炎(ゲフィチニブ、ボルテゾミブを含む)「Cancer Treatment Navigator」(中川和彦編集) (2007)
  • Author(s): 弦間昭彦
  • Publisher: メディカルビュー社(東京)
• [Book] II.肺癌の分子生物学3.癌の生物学と臨床応用の現状。肺癌のすべて(江口研二、横井香平、弦間昭彦編集) (2007)
  • Author(s): 弦間昭彦
  • Publisher: 文光堂(東京)
• [Book] IX.分子標的治療CE.薬剤性肺障害。肺癌のすべて(江口研二、横井香平、弦間昭彦 編集) (2007)
  • Author(s): 弦間昭彦
  • Publisher: 文光堂(東京)
• [Book] II疾患の病因と病態8肺の線維化と発癌 Annual Review 呼吸器 2007(工藤翔二、土屋了介、金沢実、大田健編集) (2007)
  • Author(s): 弦間昭彦
  • Publisher: 中外医学社(東京)
• [Book] 腫瘍性疾患3非小細胞肺癌の集学的治療呼吸器疾患最新の治療2007-2009(工藤翔二、中田紘一郎、貫和敏博編集) (2007)
  • Author(s): 峯岸裕司、弦間昭彦
  • Publisher: 南江堂(東京)
• [Book] 第1部5章総論1薬物療法E抗癌薬。「呼吸器専門医テキスト」(工藤翔二、中田紘一郎、永井厚志、大田健編集) (2007)
  • Author(s): 篠田欣也、弦間昭彦
  • Publisher: 南江堂(東京)
• [Book] 第2章診断法ー現況と最新の進歩肺癌診療におけるDNA診断。医学の歩み呼吸器疾患-state of arts-Ver.5.(北村諭、工藤翔二、石井芳樹編集) (2007)
  • Author(s): 岡野哲也, 弦間昭彦
  • Publisher: 医歯薬出版株式会社(東京)
• [Book] 2.薬効群別副作用14)抗がん剤d.間質性肺炎医薬品副作用学-薬品の安全使用 Update-(高橋隆一編集) (2007)
  • Author(s): 弦間昭彦
  • Publisher: 日本臨床社(東京)
• [Book] 「発見診断」肺癌を合併しやすい呼吸器疾患。MOOK「肺癌の臨床2006-2007」(監修加藤治文、西條長宏、福岡正博、小林紘一、海老原善郎、井内康輝、早川和重) (2007)
  • Author(s): 弦間昭彦
  • Publisher: 篠原出版新社(東京)