Role of toll-like receptors in alveolar epithelial injury induced by cell deformation.

• Project/Area Number: 19590920
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Kanazawa Medical University
• Principal Investigator: TOGA Hirohisa Kanazawa Medical University, 医学部, 教授 (90142554)
• Co-Investigator(Kenkyū-buntansha): UEDA Yoshimichi 金沢医科大学, 医学部, 教授 (50271375)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 呼吸器病学 / ventilator-induced lung injury / 肺胞上皮損傷 / toll-like receptor / II型肺胞上皮細胞 / 免疫組織染色 / cytokine-induced neutrophil chemoattractant-1 / DNA ladder / NF-κB / エンドトキシン / 活性窒素酸化物 / reverse transferase-polymerase chain reaction

Research Abstract

ラットを比較的大きな1回換気量(15-30ml/kg)で換気するとventilator-induced lung injury (VILI)が起こった。その時に肺胞上皮細胞にToll-like receptor-2 (TLR-2)、TLR-4の発現増強が見られ、活性窒素酸化物、CXC chemokinesの活性化を通して、NF-κB、アポトーシスを増強し肺胞上皮損傷が起こることが示唆された。

Research Products

• [Journal Article] Combined evaluation of preoperative FDG uptake on PET, GGO components on CT, and serum CEA Level: Identification of both low and high risk for recurrence in patients with resected T1 lung adenocarcinoma. (2009)
  • Author(s): Higashi K, Sakuma T, Ito K, Niho S, Ueda Y, Kobayashi T, Sekiguchi R, Takahashi T, Kato T, Tonami H
  • Journal Title: Eur J Nucl Med Molecul Imag 36 Pages: 373-381
  • Peer Reviewed
• [Journal Article] Endogenous catecholamine stimulates alveolar fluid clearance in rats with acute pancreatitis. (2009)
  • Author(s): Xu J, Wang Z, Ma G, Sagawa M, Shimazaki M, Ueda Y, Sakuma T
  • Journal Title: Respirol 14 Pages: 195-202
  • Peer Reviewed
• [Journal Article] Increased reabsorption of alveolar edema fluid in obese Zucker rats. (2008)
  • Author(s): Ma G, Zhao X, Ueno M, Tanaka M, Machida Y, Aikawa H, Usuda K, Sagawa M, Ueda Y, Sakuma T
  • Journal Title: Tohoku J Exp Med 216 Pages: 223-230
  • NAID: 10025103997
  • Peer Reviewed
• [Journal Article] Down regulation of plakoglobin in soft tissue sarcoma is associated with a higher risk of pulmonary metastasis. (2008)
  • Author(s): Kanazawa Y, Ueda Y , Shimasaki M, Katsuda S, Yamamoto N, Tomita K, Tsuchiya H
  • Journal Title: Anticancer Res 28 Pages: 655-664
  • Peer Reviewed
• [Journal Article] A mutation in Rab38 small GTPase causes abnormal lung surfactant homeostasis and aberrant alveolar structure in mice. (2008)
  • Author(s): Osanai K, Oikawa R, Higuchi J, Kobayashi M, Tsuchihara K, Iguchi M, Huang J, Toga H, Voelker DR
  • Journal Title: Am J Pathol 173 Pages: 1265-1274
  • Peer Reviewed
• [Journal Article] A mutation in Rab38 small GTPase causes abnormal lung surfactant homeostasis and aberrant alveolar structure in mice (2008)
  • Author(s): Osanai K
  • Journal Title: Am J Pathol 173 Pages: 1265-1274
  • Peer Reviewed
• [Journal Article] Chronic pulmonary artery occlusion increases alveolar fluid clearance in rats. (2007)
  • Author(s): Wang Z, Xu J, Ma G, Sagawa M, Shimazaki M, Ueda Y, Sakuma T.
  • Journal Title: J Thorac Cardiovasc Surg 134 Pages: 1213-1219
  • Peer Reviewed
• [Journal Article] Lung carcinoma: diffusion-weighted MR imaging - preliminary evaluation with apparent diffusion coefficient. (2007)
  • Author(s): Matoba M, Tonami H, Kondou T, Yokota H, Higashi K, Toga H, Sakuma T
  • Journal Title: Radiology 243 Pages: 570-577
  • Peer Reviewed
• [Journal Article] Lung carcinoma: Diffusion-weighted MR imaging-preliminary evaluation with apparent diffusion coefficient. (2007)
  • Author(s): Matoba M
  • Journal Title: Radiology 243 Pages: 570-577
  • Peer Reviewed
• [Presentation] Abnormal lung phenotype in Rab38-deficient rats, rodent models of Hermansky-Pudlak syndrome. (2009)
  • Author(s): K. Osanai, H. Toga, et al
  • Organizer: FASEB Meeting
  • Place of Presentation: New Orleans
  • Year and Date: 2009-04-19
• [Presentation] Rab38-deficient rats show phenotype of Hermansky-Pudlak synd-rome. (2007)
  • Author(s): K. Osanai, R. Oikawa, J. Higuchi, T. Miwa, H. Toga
  • Organizer: American Thoracic Society
  • Place of Presentation: San-Francisco
  • Year and Date: 2007-05-21
• [Presentation] Dobbs Leland: Expression of VEGF in alveolar type I and type II cell. (2007)
  • Author(s): 戸部勇保, 齋藤雅俊, 小島好司, 田中篤利, 舘 由貴, 及川理恵子, 中川 研, 土原一真, 井口晶晴, 関 利満, 高橋昌克, 長内和弘, 黄 正寿, 栂 博久
  • Organizer: 日本呼吸器学会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2007-05-12