Role of cytosolic phospholipase A2 in intrarenalrenin-angiotensin system

• Project/Area Number: 19590934
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: The University of Tokyo
• Principal Investigator: SEKI George The University of Tokyo, 医学部・附属病院, 講師 (30206619)
• Co-Investigator(Kenkyū-buntansha): YAMADA Hideomi 東京大学, 医学部・附属病院, 助教 (60396752) ; NOIRI Eisei 東京大学, 医学部・附属病院, 準教授 (00301820)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: アンジオテンシンII / ERK / PLA2 / 腎近位尿細管 / cPLA2

Research Abstract

本研究の目的は腎近位尿細管輸送に対するアンジオテンシンII(Ang I)の二相性作用の細胞内情報伝達系を明らかにすることである。詳細な検討により低濃度Ang IIによる刺激作用はERK 経路に依存しており、一方高濃度Ang IIによる阻害作用はERK 経路には依存せずグループIV細胞内ホスホリパーゼA2/アラキドン酸代謝経路に依存することが示された。さらに腎近位尿細管では細胞内ホスホリパーゼA2/アラキドン酸代謝経路はERK経路に対して抑制的に働くことが明らかになった。

Research Products

• [Journal Article] Roles ofMEK/ERK pathway in vascular and renaltubular actions of angiotensin II (2009)
  • Author(s): Seki G, Yamada H, Li Y, Horita S, Ishizaka N, Koike K and Fujita T
  • Journal Title: Vascular Disease Prevention 6 Pages: 154-159
  • Peer Reviewed
• [Journal Article] Arachidonic acid metabolitesinhibit the stimulatory effect of angiotensin II in renal proximal tubules (2008)
  • Author(s): Li Y, Yamada H, Kita Y, Suzuki M, EndoY, Horita S, Yamazaki O, Shimizu T, SekiG, Fujita T
  • Journal Title: Hypertension Res 31 Pages: 2155-2164
  • Peer Reviewed
• [Journal Article] Activation and Inactivation Mechanisms of Na-HCO3 Cotransporter NBC1 (2008)
  • Author(s): Seki G, Yamada H, Horita S, Suzuki M, Sekine T, Igarashi T and Fujita T
  • Journal Title: J Epithelial Biology and Pharmacology 1 Pages: 35-39
  • Peer Reviewed
• [Journal Article] The Roles ofAbnormal Renal Sodium Handling inHypertension Associated with MetabolicSyndrome (2008)
  • Author(s): Seki G, Yamada H, Li Y, Horita S, Suzuki M and Fujita T
  • Journal Title: Current Hypertension Reviews 4 Pages: 197-202
  • Peer Reviewed
• [Journal Article] Roles of ERK and cPLA2in the angiotensin II-mediated biphasicregulation of Na + - HCO3 - transport (2008)
  • Author(s): Li Y, Yamada H, Kita Y, Kunimi M, Horita S, Suzuki M, Endo Y, Shimizu T,Seki G, Fujita T
  • Journal Title: J Am Soc Nephrol 19 Pages: 252-259
• [Journal Article] Functionalanalysis of a novel missense NBC1mutation and of other mutations causingproximal renal tubular acidosis (2008)
  • Author(s): Suzuki M, Vaisbich M, Yamada H,Horita S, Li Y, Sekine T, Moriyama N,Igarashi T, Endo Y, Cardoso T, de Sa L,Koch H, Seki G, Fujita, T
  • Journal Title: Pflugers Arch 455 Pages: 583-593
  • Peer Reviewed
• [Journal Article] Association of CigaretteSmoking with Chronic Kidney Disease in Japanese men (2008)
  • Author(s): Ishizaka N, Ishizaka Y, Toda E,Shimomura H, Koike K, Seki G, Nagai R, Yamakado M
  • Journal Title: Hypertension Res 31 Pages: 485-492
  • Peer Reviewed
• [Journal Article] The Roles of Abnormal Renal Sodium Handling in Hypertension Associated with Metabolic Syndrome (2008)
  • Author(s): Seki G et al.
  • Journal Title: Current Hypertension Reviews 4 Pages: 197-202
  • Peer Reviewed
• [Journal Article] Activation and Inactivation Mechanisms of Na-HCO3 Cotransporter NBCl (2008)
  • Author(s): Seki G et al.
  • Journal Title: J Epithelial Biology and Pharmacology 1 Pages: 35-39
  • Peer Reviewed
• [Journal Article] Arachidonic acid metabolites inhibit the stimulatory effect of angiot ensin II in renal proximal tubules (2008)
  • Author(s): Li Y et al.
  • Journal Title: Hypertension Res 31 Pages: 2155-2164
  • Peer Reviewed
• [Journal Article] Roles of ERK and cPLA2 in the angiotensin II-mediated biphasic regulation of Na+-HCO3-transport (2008)
  • Author(s): Li Y, et. al.
  • Journal Title: J Am Soc Nephrol 19 Pages: 252-259
  • Peer Reviewed
• [Journal Article] Functional analysis of a novel missense NBC1 mutation and of other mutations causing proximal renal tubular acidosis (2008)
  • Author(s): Suzuki M, et. al.
  • Journal Title: Pflugers Arch 455 Pages: 583-593
  • Peer Reviewed
• [Journal Article] Interaction ofmembrane skeletal protein, protein 4.1Band p55 and sodium-bicarbonatecotransporter1 in mouse renal S1-S2proximal tubules (2007)
  • Author(s): Terada N, Ohno N, Saitoh S, Seki G, Komada M, Suzuki T, Yamakawa H, Soleimani M, Ohno S
  • Journal Title: J Histochem Cytochem 55 Pages: 1199-1206
  • Peer Reviewed
• [Journal Article] Associationbetween Obesity and Chronic KidneyDisease in Japanese : Differences in Genderand Hypertensive Status? (2007)
  • Author(s): Ishizaka N, Ishizaka Y, Koike K, Seki G, Nagai R, Yamakado M
  • Journal Title: Hypertension Res 30 Pages: 1059-1064
  • Peer Reviewed
• [Journal Article] Association between Chronic KidneyDisease and Carotid Intima-MediaThickening in Individuals withHypertension and Impaired GlucoseMetabolism (2007)
  • Author(s): Ishizaka N, Ishizaka Y, Toda E, KoikeK, Seki G, Nagai R, Yamakado M
  • Journal Title: Hypertension Res 30 Pages: 1035-1041
  • Peer Reviewed
• [Journal Article] Interaction of membrane skeletal protein, protein 4.1B and p55 and sodium-bicarbonate cotransporterl in mouse renal S1-S2 proximal tubules (2007)
  • Author(s): Terada N, et. al.
  • Journal Title: J Histochem Cytochem 55 Pages: 1199-1206
  • Peer Reviewed
• [Presentation] PPARg/EGFR/ERK経路を介したチアゾリンの近位尿細管作用 (2008)
  • Author(s): 遠藤陽子、山田秀臣、李月紅、鈴木正志、山崎修、門脇孝、関常司、藤田敏郎
  • Organizer: 第51回日本腎臓学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2008-06-01
• [Presentation] Physiology and pathophysiology of Na-HCO3 cotransporter NBC1 (2008)
  • Author(s): Seki G, H Yamada
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-25
• [Presentation] 酸・塩基平衡関連分子の臨床 (2007)
  • Author(s): 関常司、堀田晶子、鈴木正志、山田秀臣
  • Organizer: 第37回日本腎臓学会東部会
  • Place of Presentation: 埼玉
  • Year and Date: 2007-10-06
• [Presentation] 酸・塩基平衡関連分子の臨床 (2007)
  • Author(s): 関 常司
  • Organizer: 日本腎臓学会東部会
  • Place of Presentation: 大宮ソニックシティ
  • Year and Date: 2007-10-06