Targeting the brap2, a candidate gene for ataxia caused by developmental defects of the cerebellum

• Project/Area Number: 19591014
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurology
• Research Institution: Nippon Medical School
• Principal Investigator: ASADA Minoru Nippon Medical School, 医学部, 講師 (60366755)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Hidenori 日本医科大学, 大学院・医学研究科, 教授 (30221328)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 神経分子病態学 / 細胞周期 / 遺伝子ターゲティング / Gene trap / brap2 / p21 / 核移行 / 細胞質 / 核移行シグナル

Research Abstract

小脳発達障害候補遺伝子BRAP2の生化学的解析をおこない、BRAP2は現在までに知られているプロテアソームによるタンパク質分解系とは異なる様式でポリユビキチン化することを見出した。さらに個体レベルでの機能解析を目指してGene trap 法によるマウスES細胞のデータベースより、brap2遺伝子の領域にターゲティングされたクローンを見つけた。ノックアウトマウスを作成する目的でヘミ接合子マウスを作製した。

Research Products

• [Journal Article] Involvement of neural cell adhesion molecule signaling in glial cell line-derived neurotrophic factor-induced analgesia in a rat model of neuropathic pain. (2008)
  • Author(s): Sakai, A., Asada, M., Seno, N., Suzuki, H.
  • Journal Title: Pain 137 Pages: 378-388
  • Peer Reviewed
• [Journal Article] The prolonged analgesic effect of epidural ropivacaine in a rat model of neuropathic pain (2008)
  • Author(s): Sato, C., Sakai, A., Ikeda, Y., Suzuki, H., Sakamoto, A.
  • Journal Title: Anesth. Analg. 106 Pages: 313-320
  • Peer Reviewed
• [Journal Article] Prenatal dexamethasone exposure affects anxiety-like behaviour and neuroendocrine systems in an age-dependent manner (2008)
  • Author(s): Nagano, M., Ozawa, H., Suzuki, H.
  • Journal Title: Neurosci. Res. 60 Pages: 364-371
  • Peer Reviewed
• [Journal Article] Low serum levels of brain-derived neurotrophic factor and epidermal growth factor in patients with chronic schizophrenia. (2008)
  • Author(s): Ikeda, Y., Yahata, N., Ito, I., Nagano, M., Toyota, T., Yoshikawa, T., Okubo, Y., Suzuki, H.
  • Journal Title: Schizophr. Res. 101 Pages: 58-66
  • Peer Reviewed
• [Journal Article] Chronic fluoxetine bidirectionally modulates potentiating effects of serotonin on the hippocampal mossy fiber synaptic transmission. (2008)
  • Author(s): Kobayashi, K., Ikeda, Y., Haneda, E., Suzuki, H.
  • Journal Title: J Neurosci. 28 Pages: 6272-6280
  • Peer Reviewed
• [Journal Article] Increase in hemokinin-1 mRNA in the spinal cord during the early phase of a neuropathic pain state. (2008)
  • Author(s): Matsumura, T., Sakai, A., Nagano, M., Sawada, M., Suzuki, H., Umino, M., Suzuki, H.
  • Journal Title: Br. J. Pharmacol. On line
  • Peer Reviewed
• [Journal Article] Alpha-CaMKII deficiency causes immature dentate gyrus, a novel candidate endophenotype of psychiatric disorders. (2008)
  • Author(s): Yamasaki, N., Maekawa, M., Kobayashi, K., Kajii, Y., Maeda, J., Soma, M., Takao, K., Tanda, K., Ohira, K., Toyama, K., Kanzaki, K., Fukunaga, K., Sudo, Y., Ichinose, H., Ikeda, M., Iwata, N., Ozaki, N., Suzuki, H., Higuchi, M., Suhara, T., Yuasa, S., Miyakawa, T.
  • Journal Title: Mol Brain 1 Pages: 6-6
  • Peer Reviewed
• [Journal Article] Foot shock stress prolongs the telogen stage of the spontaneous hair cycle in a non-depilated mouse model. (2007)
  • Author(s): Katayama, M., Aoki, E., Suzuki, H., Kawana, S.
  • Journal Title: Exp. Dermatol. 16 Pages: 553-560
  • Peer Reviewed