Role of Perilipin in Adipocyte Lipolysis and Metabolic Disease

• Project/Area Number: 19591029
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Hokkaido University
• Principal Investigator: MIYOSHI Hideaki Hokkaido University, 北海道大学病院, 助教 (30360902)
• Co-Investigator(Kenkyū-buntansha): SHIMIZU Chikara 北海道大学, 北海道大学病院, 講師 (00292029)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 代謝異常症 / 肥満症 / ペリリピン / 肥満 / 脂質代謝 / 脂肪細胞 / 代謝学 / 糖尿病 / 脂肪分解 / マウス

Research Abstract

定常状態での脂肪細胞の脂肪貯留と分解の程度や脂肪細胞の大きさについて、それらの制御に関与していると考えられる3種類の蛋白(ぺリリピン、HSL、ATGL)の相対的寄与度と相関性を分子学的手法を用いて明らかにし、英文雑誌に掲載された。また、ぺリリピンを過剰発現させた遺伝子改変マウスを作成し、それが痩せていて糖代謝改善などを来すことを発見し、ぺリリピン量の変化が生体内での様々な代謝変化と関係することを明らかにした。

Research Products

• [Journal Article] Adipose triglyceride lipase regulates basal lipolysis and lipid droplet size in adipocytes (2008)
  • Author(s): Miyoshi H, Perfield JM, Obin MS, et.al.(4人中1番目)
  • Journal Title: J Cell Biochem 15 ; 105(6) Pages: 1430-6
  • Peer Reviewed
• [Journal Article] AMP-activated protein kinase (AMPK) is activated as a consequence of lipolysis in the adipocyte: potential mechanism and physiological relevance (2008)
  • Author(s): Gautier MS, Miyoshi H, Souza SC, et.al.(7人中2番目)
  • Journal Title: J Biol Chem 283(24) Pages: 16514-24
  • Peer Reviewed
• [Journal Article] Adipose triglyceride lipase regulates basal lipolysis and lipid droplet size in adipocytes (2008)
  • Author(s): Miyoshi H
  • Journal Title: J Cell Biochem 105 Pages: 1430-1436
  • Peer Reviewed
• [Journal Article] AMP-activated protein kinase (AMPK) is activated as a consequ ence of lipolysis in the adipocyte : potential mechanism and phy siological relevance (2008)
  • Author(s): Gauthier MS
  • Journal Title: J Biol Chem 283 Pages: 16514-16524
  • Peer Reviewed
• [Journal Article] Control of ATGL action by serine 517 of perilipin A globally regulates PKA-stimulated lipolysis in adipocytes (2007)
  • Author(s): Miyoshi H, Perfield JM, Souza SC, et.al.(9人中1番目)
  • Journal Title: J Biol Chem 282(2) Pages: 996-1002
  • Peer Reviewed
• [Journal Article] Adipocyte death, adipose tissue remodeing and obesity complications (2007)
  • Author(s): Strissel KJ, Stancheva Z, Miyoshi H, et.al.(7人中3番目)
  • Journal Title: Diabetes 56(12) Pages: 2910-8
  • Peer Reviewed
• [Journal Article] Perilipin regulates the thermogenic actions of norepinephrine in brown adipose tissue (2007)
  • Author(s): Souza SC, Christoffolete MA, Miyoshi H, et.al.(12人中4番目)
  • Journal Title: J Lipid Res 48(6) Pages: 1273-9
  • NAID: 120006352221
  • Peer Reviewed
• [Journal Article] 脂肪細胞内におけるPerilipinの脂肪分解制御機構と肥満・代謝疾患 (2007)
  • Author(s): 三好秀明
  • Journal Title: リポ蛋白・代謝研究会誌 37巻 Pages: 11-14
• [Journal Article] メタボリックシンドロームの基礎 (2007)
  • Author(s): 三好秀明
  • Journal Title: 北海道医報 1071巻 Pages: 26-31
• [Journal Article] Control of ATGL action by serine 517 of perilipin A globally regulates PKA-stimulated lipolysis in adipocytes. (2007)
  • Author(s): Miyoshi H
  • Journal Title: J Biol Chem 282 Pages: 996-1002
  • Peer Reviewed
• [Journal Article] Adipocyte death, adipose tissue remodeling and obesity complications. (2007)
  • Author(s): Strissel KJ
  • Journal Title: Diabetes 56 Pages: 2910-2918
  • Peer Reviewed
• [Journal Article] Perilipin regulation of adaptive thermogenesis in brown adipose tissue (2007)
  • Author(s): Souza SC
  • Journal Title: J Lipid Res 48 Pages: 1273-1279
  • Peer Reviewed
• [Journal Article] 脂肪細胞内におけるPerilipinの脂肪分解制御機構と肥満・代謝疾患 (2007)
  • Author(s): 三好 秀明
  • Journal Title: リポ蛋白・代謝研究会誌 37 Pages: 11-14
• [Journal Article] Perilipin promotes hormone-sensitive lipase-mediated adipocyte lipolysis via phosphorylation-dependent and -independent mechanisms (2006)
  • Author(s): Miyoshi H, Souza SC, Zhang HH, et.al.(11人中1番目)
  • Journal Title: J Biol Chem 281(23) Pages: 15837-44
  • NAID: 120000970417
  • Peer Reviewed
• [Journal Article] Dynamics of lipid droplet associated proteins during hormonally stimulated lipolysis in engineered adipocytes : Stabilization and lipid droplet binding of ADRP/adipophilin (2006)
  • Author(s): Gross DN, Miyoshi H, Hosaka T, et.al.(9人中2番目)
  • Journal Title: Mol Endocrinol Feb ; 20(2) Pages: 459-66
  • Peer Reviewed
• [Presentation] 脂肪滴周囲蛋白perilipinの脂肪分解制御部位(アミノ酸)の同定と脂肪分解酵素ATGLとの関与 (2007)
  • Author(s): 三好 秀明
  • Organizer: 第50回日本糖尿病学会年次学術集会
  • Place of Presentation: 仙台
  • Year and Date: 2007-05-25
• [Presentation] 脂肪滴周囲蛋白perilipinの脂肪分解制御部位(アミノ酸)の同定と脂肪分解酵素ATGLとの関与 (2007)
  • Author(s): 三好秀明
  • Organizer: 第50回日本糖尿病学会年次学術集会
  • Place of Presentation: 仙台市
• [Remarks]
  • URL: http://www.med.hokudai.ac.jp/~med-2w