Early detection and diagnosis of fulminant type 1 diadetes mellitus

• Project/Area Number: 19591041
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Metabolomics
• Research Institution: University of Yamanashi
• Principal Investigator: ENDO Toyoshi University of Yamanashi, 大学院・医学工学総合研究部, 准教授 (00152017)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Tetsuro 山梨大学, 大学院・医学工学総合研究部, 教授 (30113442) ; KANESHIGE Masahiro 山梨大学, 医学部・附属病院, 助教 (20377518)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 劇症1型糖尿病 / 自己免疫性膵炎 / アミラーゼ / 自己抗体 / cDNA / 1型糖尿病

Research Abstract

アミラーゼ自己抗体を検出するELISA を確立し、種々の膵疾患での陽性率を検討した。その結果劇症1型糖尿病では88%で陽性であったが、2型糖尿病では6%に留まった。一方、自己免疫性膵炎でもほぼ100%で陽性であった。膵腫瘍、アルコール性膵炎患者等ではほぼ全例陰性であることが判明した。以上の結果はアミラーゼ自己抗体は劇症1型糖尿病および自己免疫性膵炎に特異的に産生され、両疾患の診断に有用であることが示された。

Research Products

• [Journal Article] lase α-2A Autoantibod ies : Novel Marker of Autoimmune Pancre atitis and Fulminant Type 1 Diabetes Mell itus (2009)
  • Author(s): Endo T, et.al
  • Journal Title: Diabetes 58 Pages: 732-777
  • Peer Reviewed
• [Journal Article] Amylase α-2A Autoantibodies : Novel Marker of Autoimmune Pancreatitis and Fulminant Type 1 Diabetes Mellitus. (2009)
  • Author(s): Endo T, et al.
  • Journal Title: Diabetes 58 Pages: 732-77
  • Peer Reviewed
• [Journal Article] Insulin intervention in slowly progressive insulin-dependent (type 1) diabetes mellitus. (2008)
  • Author(s): Maruyama T, et al.
  • Journal Title: J Clin Endocrinol Metab. 93 Pages: 2115-2121
  • Peer Reviewed
• [Journal Article] Japanese Study Group on Type 1 Diabetes Genetics. Evidence for association between vitamin D receptor BsmI polymorphism and type 1 diabetes in Japanese (2008)
  • Author(s): Shimada A, et al.
  • Journal Title: J Autoimmun 30 Pages: 207-211
  • Peer Reviewed
• [Journal Article] HSP 10 is a new autoantigen in both autoimmune pancreatitis and fulminant type 1 diabetes
  • Author(s): S.Takizawa, T. Endo, Xing Wanjia, S. Tanaka, M. Takahashi, T. Kobayashi
  • Journal Title: Biochem Biophys Res Commun (in press)
  • Peer Reviewed
• [Presentation] Novel autoantibody against heat shock protein 10 in diabetes associated with autoimmune pancreatitis and fulminant type 1 diabetes (2008)
  • Author(s): Souichi Takizawa, Toyoshi Endo, Shoichiro Tanaka, Masashi Takahashi, Tetsuro Kobayashi
  • Organizer: 68th Scientific Sessions of American Diabetes Association
  • Place of Presentation: Chicago. USA
  • Year and Date: 2008-06-16
• [Presentation] 自己免疫性膵炎と劇症1型糖尿病に検出される膵外分泌抗体の対応抗原 (2008)
  • Author(s): 滝澤壮一、遠藤登代志、田中昌一郎、小林哲郎
  • Organizer: 第51回日本糖尿病学会年次学術集会
  • Place of Presentation: 東京
  • Year and Date: 2008-05-23