Molecular mechanism of chaperone properties in bone metabolism

• Project/Area Number: 19591042
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Gifu University
• Principal Investigator: KOZAWA Osamu Gifu University, 大学院・医学系研究科, 教授 (90225417)
• Co-Investigator(Kenkyū-buntansha): TOKUDA Haruhiko 岐阜大学, 大学院・医学系研究科・非常勤, 講師 (10397325) ; 高井 信治 岐阜大学, 大学院・医学系研究科, 助教 (60402153)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ストレス蛋白質 / マップキナーゼ / 骨芽細胞 / カテキン / ポリフェノール

Research Abstract

ストレス蛋白質は、熱や化学物質などのストレスにより組織・細胞内に誘導される一群のタンパク質の総称である。低分子量ストレス蛋白質がどのようなメカニズムで、骨代謝を調節しているのかについては不明である。本研究では、骨芽細胞において、低分子量ストレス蛋白質のHSP27誘導の分子機序の一部を明らかとした。本結果は、骨粗鬆症に対する薬物治療法への応用に極めて重要かつ有用であると考えられる。

Research Products

• [Journal Article] Tacrolimus but not cyclosporine A enhances FGF-2-induced VEGF release in osteoblasts (2009)
  • Author(s): Yamauchi J, Takai S, Matsushima-Nishiwaki R, Adachi S, Minamitani C, Natsume H, Mizutani J, Otsuka T, Takeda J, Harada A, Kozawa O, Tokuda H.
  • Journal Title: Int. J. Mol. Med. 23 Pages: 267-272
  • NAID: 120006340794
  • Peer Reviewed
• [Journal Article] Involvement of Rho-kinase in sphingosine 1-phosphate-stimulated HSP27 induction in osteoblasts (2009)
  • Author(s): Minamitani C, Otsuka T, Takai S, Matsushima Nishiwaki R, Adachi S, Hanai Y, Mizutani J, Tokuda H, Kozawa O.
  • Journal Title: Int. J. Mol. Med. 24 Pages: 77-82
  • NAID: 120006340793
  • Peer Reviewed
• [Journal Article] Rho-kinase inhibitors decrease TGF-β-stimulated VEGF synthesis through stress-activated protein kinase/c-Jun N-terminal kinase in osteoblasts (2009)
  • Author(s): Kuno M, Takai S, Matsushima-Nishiwaki R, Minamitani C, Mizutani J, Otsuka T, Harada A, Adachi S, Kozawa O, Tokuda H.
  • Journal Title: Bicohem. Pharmacol 77 Pages: 196-203
  • Peer Reviewed
• [Journal Article] Function of Rho-kinase in prostaglandin D_2-induced interleukin-6 synthesis in osteoblasts (2008)
  • Author(s): Tokuda H, Takai S, Matsushima-Nishiwaki R, Hanai Y, Adachi S, Minamitani C, Mizutani J, Otsuka T, Kozawa O
  • Journal Title: Prostaglandins Leukot. Essent. Fatty Acids 79 Pages: 41-46
  • Peer Reviewed
• [Journal Article] (-)-Epigallocatechin gallate reduces platelet-derived growth factor BB-stimulated interleukin-6 synthesis in osteoblasts : suppression of SAPK/JNK (2008)
  • Author(s): Takai S, Matsushima-Nishiwaki R, Adachi S, Natsume H, Minamitani C, Mizutani J, Otsuka T, Tokuda H, Kozawa O
  • Journal Title: Mediators Inflamm 2008
  • Peer Reviewed
• [Journal Article] Involvement of Rho-kinase in prostaglandin F2α stimulated interleukin-6 synthesis via p38 mitogen-activated protein kinase in osteoblasts (2008)
  • Author(s): Minamitani C, Otsuka T, Takai S, Matsushima Nishiwaki R, Adachi S, Hanai Y, Mizutani J, Tokuda H, Kozawa O.
  • Journal Title: Mol. Cell. Endocrinol 291 Pages: 27-32
  • Peer Reviewed
• [Journal Article] (-)-Epigallocatechin gallate reduces transforming growth factor β stimulated HSP27 induction through the suppression of stress activated protein kinase/c-Jun N-terminal kinase in osteoblasts (2008)
  • Author(s): Hayashi K, Takai S, Matsushima-Nishiwaki R, Hanai Y, Kato K, Tokuda H, Kozawa O.
  • Journal Title: Life Sci. 82 Pages: 1012-1017
  • Peer Reviewed
• [Journal Article] p70 S6 kinase negatively regulates FGF-2-stimulated IL-6 synthesis in osteoblasts: function at a point downstream from protein kinase C (2008)
  • Author(s): Takai S, Hanai Y, Matushima-Nishiwaki R, Minamitani C, Otsuka T, Tokuda H, Kozawa O.
  • Journal Title: J. Endocrinol 197 Pages: 131-137
  • Peer Reviewed
• [Journal Article] A case of raloxifene-induced acceleration of platelet aggregation (2008)
  • Author(s): Minamitani C, Takai S, Matsushima-Nishiwaki R, Hanai Y, Otsuka T, Kozawa O, Tokuda H.
  • Journal Title: Internal Med. 47 Pages: 1523-1528
  • Peer Reviewed
• [Journal Article] (-)-Epigallocatechin gallate inhibits basic fibroblast growth factor induced interleukin-6 synthesis in osteoblasts (2008)
  • Author(s): Tokuda H, Takai S, Hanai Y, Matsushima-Nishiwaki R, Yamauchi J, Harada A, Hosoi T, Ohta T, Kozawa O.
  • Journal Title: Horm. Metab. Res. 40 Pages: 674-678
  • Peer Reviewed
• [Journal Article] Potentiation by platelet-derived growth factor-BB of FGF-2-stimulated VEGF release in osteoblasts (2008)
  • Author(s): Tokuda H, Takai S, Hanai Y, Harada A, Matsushima Nishiwaki R, Kato H, Ogura S, Kozawa O.
  • Journal Title: J. Bone Miner. Metab. 26 Pages: 335-341
  • NAID: 10024458639
  • Peer Reviewed
• [Journal Article] (-)-Epigallocatechin gallate reduces transforming growth factor β-stimulated HSP27 induction through the suppression of stress-activated protein kinase/c-Jun N-terminal kinase in osteoblasts. (2008)
  • Author(s): Hayashi K, Kozawa O, et. al.
  • Journal Title: Life Sci. (in press)
  • Peer Reviewed
• [Journal Article] Platelet-derived growth factor-BB amplifies PGF_<2α>-induced VEGF synthesis in osteoblasts: function of phosphatidylinositol 3-kinase (2007)
  • Author(s): Tokuda H, Takai S, Hanai Y, Harada A, Matsushima-Nishiwaki R, Akamatsu S, Ohta T, Kozawa O
  • Journal Title: Prostaglandins Leukot. Essent. Fatty Acids. 77 Pages: 187-193
  • Peer Reviewed
• [Journal Article] (-)-Epigallocatechin gallate inhibits prostaglandin D_2-stimulated HSP27 induction via suppression of the p44/p42 MAP kinase pathway in osteoblasts (2007)
  • Author(s): Yamauchi J, Takai S, Matsushima-Nishiwaki R, Hanai Y, Doi T, Kato H, Ogura S, Kato K, Tokuda H, Kozawa O.
  • Journal Title: Prostaglandins Leukot. Essent. Fatty Acids 77 Pages: 173-179
  • Peer Reviewed
• [Journal Article] Rho-kinase regulates endothelin-1-stimulated IL-6 synthesis via p38 MAP kinase in osteoblasts (2007)
  • Author(s): Tokuda H, Hanai Y, Matsushima-Nishiwaki R, Yamauchi J, Doi T, Harada A, Takai S, Kozawa O.
  • Journal Title: Biochem. Biophys. Res. Commun 362 Pages: 799-804
  • Peer Reviewed
• [Journal Article] (-)-Epigallocatechin gallate suppresses endothelin-1-induced interleukin-6 synthesis in osteoblasts: inhibition of p44/p42 MAP kinase activation (2007)
  • Author(s): Tokuda H, Takai S, Hanai Y, Matsushima-Nishiwaki R, Hosoi T, Harada A, Ohta T, Kozawa O.
  • Journal Title: FEBS Lett. 581 Pages: 1311-1316
  • Peer Reviewed
• [Journal Article] Negative regulation by p70 S6 kinase of FGF-2-stimulated VEGF release through stress-activated protein kinase/c-Jun N-terminal kinase in osteoblasts (2007)
  • Author(s): Takai S, Tokuda H, Hanai Y, Harada A, Yasuda E, Matsushima-Nishiwaki R, Kato H, Ogura S, Ohta T, Kozawa O
  • Journal Title: J. Bone Miner. Res. 22 Pages: 337-346
  • Peer Reviewed
• [Journal Article] Activation of phosphatidylinositol 3-kinase/Akt limits FGF-2-induced VEGF release in osteoblasts (2007)
  • Author(s): Takai S, Tokuda H, Hanai Y, Kozawa O.
  • Journal Title: Mol. Cell. Endocrinol 267 Pages: 46-54
  • Peer Reviewed
• [Journal Article] Limitation by p70 S6 kinase of PDGF-BB-induced IL-6 synthesis in osteoblast-like MC3T3-E1 cells (2007)
  • Author(s): Takai S, Tokuda H, Hanai Y, Kozawa O.
  • Journal Title: Metabolism 56 Pages: 476-483
  • Peer Reviewed
• [Journal Article] (-)-Epigallocatechin gallate enhances prostaglandin F_<2α>-induced VEGF synthesis via up-regulating SAPK/JNK activation in osteoblasts (2007)
  • Author(s): Tokuda H, Takai S, Matsushima-Nishiwaki R, Akamatsu S, Hanai Y, Hosoi T, Harada A, Ohta T, Kozawa O.
  • Journal Title: J. Cell. Biochem 100 Pages: 1146-1153
  • Peer Reviewed
• [Journal Article] (-)-Epigallocatechin gallate inhibits prostaglandin D_2-stimulated HSP27 induction via suppression of the p44/p42 MAP kinase pathway in osteoblasts. (2007)
  • Author(s): Yamauchi J, Kozawa O, et. al.
  • Journal Title: Prostaglandins Leukot. Essent. Fatty Acids 77 Pages: 173-179
  • Peer Reviewed
• [Remarks]
  • URL: http://www.med.gifu-u.ac.jp/pharma/index.htm
• [Remarks]
  • URL: http://www.med.gifu-u.ac.jp/pharma/index.htm