Elucidation of mechanism of glucose response in islet beta-cells by analyzing stability control system of transcription factor MafA.

• Project/Area Number: 19591078
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Endocrinology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: KATAOKA Kohsuke Nara Institute of Science and Technology, バイオサイエンス研究科, 准教授 (20262074)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 転写制御 / シグナル伝達 / 糖尿病

Research Abstract

グルコース応答性でβ細胞の諸機能の発揮に必須の転写因子MafAの制御機構の解明を通じて膵島β細胞がグルコース濃度を感知して応答する分子機構の解明を目指した. MafAタンパク質がリン酸化依存的に低グルコース条件下で分解が促進されること、MafAの分解はPA28γタンパク質によって促進されることを明らかにした. この分解促進はこれまでに知られていない新規の分解系であった. また、MafAのリン酸化依存的に結合する新規因子を同定した.

Research Products

• [Journal Article] Presentation of functional foreign peptide on the surface of SV40 vilus-like particles (2008)
  • Author(s): Takahashi, R.U., S.N. Kanesashi, T. Inoue, T. Enomoto, M.A. Kawano, H. Tsukamoto, F. Takeshita, T. Imai, T. Ochiya, K. Kataoka, Y. Yamaguchi, and H. Handa
  • Journal Title: J. Biotech 135 Pages: 385-392
  • Peer Reviewed
• [Journal Article] Engineering of SV40-based nano-capsules for delivery of heterologous proteins as fusions with the minor capsid proteins VP2/3 (2008)
  • Author(s): Inoue, T., M.A. Kawano, R. U. Takahashi, H. Tsukamoto, T. Enomoto, T. Imai, K. Kataoka, and H. Handa
  • Journal Title: J. Biotech 132 Pages: 181-192
  • Peer Reviewed
• [Journal Article] Engineering of SV40-based nano-capsules for delivery of heterologous proteins as fusions with the minor capsid proteins VP2/3. (2008)
  • Author(s): Inoue, T.
  • Journal Title: J. Biotech. 132 Pages: 181-192
  • Peer Reviewed
• [Journal Article] Presentation of funct ional foreign pept ide on the surface of SV40 vilus-like particles. (2008)
  • Author(s): Takahashi, R. U.
  • Journal Title: J. Biotech. 135 Pages: 385-392
  • Peer Reviewed
• [Journal Article] Atrazine binds to F1FO-ATP synthase and inhibits mitochondrial function in sperm (2007)
  • Author(s): Hase Y., M. Tatsuno, T. Nishi, K. Kataoka, Y. Kabe, Y. Yamaguchi, N. Ozawa, M. Natori, H. Handa, and H. Watanabe
  • Journal Title: Biochem. Biophys. Res. Commun 366 Pages: 66-72
  • Peer Reviewed
• [Journal Article] Roles and regulation of transcription factor MafA in islet β-cells (2007)
  • Author(s): Aramata, S., S. -i. Han, and K. Kataoka
  • Journal Title: Endocr. J. 54 Pages: 659-666
• [Journal Article] Evidence that SV40 VP1-DNA interactions contribute to the assembly of 40-nm spherical viral particles (2007)
  • Author(s): Tsukamoto H., M.A. Kawano, T. Inoue, T. Enomoto, R.-u. Takahashi, N. Yokoyama, N. Yamamoto, T. Imai, K. Kataoka, Y. Yamaguchi, and H. Handa
  • Journal Title: Genes Cells 12 Pages: 1267-1279
  • Peer Reviewed
• [Journal Article] Atrazine binds to F1F0-ATP synthase and inhibits mitochondrial function in sperm. (2007)
  • Author(s): Hase Y.
  • Journal Title: Biochem. Biophys. Res. Commun. 366 Pages: 66-72
  • Peer Reviewed
• [Journal Article] Roles and regulation of transcription factor MafA in islet β-cells. (2007)
  • Author(s): Aramata, S.
  • Journal Title: Endocr. J. 54 Pages: 659-666
• [Journal Article] Evidence that SV40 VP1-DNA interactions contribute to the assembly of 40-nm spherical viral particles. (2007)
  • Author(s): Tsukamoto H.
  • Journal Title: Genes Cells 12 Pages: 1267-1279
  • Peer Reviewed
• [Presentation] Maf転写因子群の多重なリン酸化を介した複数の分解経路 (2008)
  • Author(s): 片岡浩介、金井賢一
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] 転写因子Snai1のPA28γによるタンパク質安定性制御 (2008)
  • Author(s): 片上紗世, 金井賢一, 片岡浩介
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] 転写因子SnailのPA28γによるタンパク質安定性制御 (2008)
  • Author(s): 片上紗世
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Book] 分子糖尿病学の進歩2009 (分担)〜膵β細胞の分化と機能維持を司る転写因子MafA (2009)
  • Author(s): 片岡浩介
  • Publisher: 金原出版
• [Book] 「膵β細胞の分化と機能維持を司る転写因子MafA」(分子糖尿病学の進歩2009(分担))
  • Publisher: 金原出版