Analysis of the expression of the brain-specific aromatase gene for the sexual dimorphic nucleus in the brain

• Project/Area Number: 19591086
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Endocrinology
• Research Institution: Fujita Health University
• Principal Investigator: HONDA Shinichiro Fujita Health University, 薬学部, 准教授 (40257639)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ステロイドホルモン / 転写因子 / 中枢神経 / 転写調節 / 中枢神経系

Research Abstract

The aromatase has a critical role in sexual differentiation of the brain. To clarify neuronal-specific, sexual-specific, and spatiotemporal expression mechanisms of aromatase gene, I analyzed brain-specific promoter region of the gene. I identified several transcription factors that act on the cis-elements in the brain-specific promoter region. Furthermore, I also determined the aromatase-expressing neurons in central nervous system using transgenic mice. In this study, I have found that important information of resolution for the construction on the sexual differentiation of the brain in mammal.

Research Products

• [Journal Article] Regulation of progestin receptors in medial amygdala: estradiol, phytoestrogens and sex. (2009)
  • Author(s): Kudawa A.E., Harada N., Honda S-I., Rissman E.F.
  • Journal Title: Physiol. Behav. 97 Pages: 146-150
  • Peer Reviewed
• [Journal Article] Estrogen masculinizes neural pathways and sex-specific behaviors. (2009)
  • Author(s): Wu, M.V., Manoli, D.S., Fraser, E.J., Coats, J.K., Tollkuhn, J., Honda, S., Harada, N., Shah, N.M.
  • Journal Title: Cell 139 Pages: 61-72
  • Peer Reviewed
• [Journal Article] Functional analysis of neurosteroidal oestrogen using gene-disrupted and transgenic mice. (2009)
  • Author(s): Harada N., Wakatsuki T., Aste N., Yoshimura N., Honda S.
  • Journal Title: J. Neuroendocrinol 21 Pages: 365-369
  • Peer Reviewed
• [Journal Article] Functional analysis of neurosteroidal oestrogen using gene-disrupted and transqenic mice (2009)
  • Author(s): Harada, N., et al.
  • Journal Title: J Neuroendocrinol 21 Pages: 365-369
  • Peer Reviewed
• [Journal Article] Regulation of progestin receptors in medial amygdala : estradiol, phytoestrogens and sex (2009)
  • Author(s): Kudwa, A.E., et al.
  • Journal Title: Physiol Behav 97 Pages: 146-150
  • Peer Reviewed
• [Journal Article] Estrogen masculinizes neural pathways and sex-specific behaviors (2009)
  • Author(s): Wu, M.V., et al.
  • Journal Title: Cell 139 Pages: 61-72
  • Peer Reviewed
• [Journal Article] Acute effects of estrogen on the guinea pig and human IKr channels and drug-induced prolongation of cardiac repolarization (2008)
  • Author(s): Kurokawa J., Tamagawa M, Harada N., Honda S, I., Bai C, X,, Nakaya H., Furukawa T
  • Journal Title: J. Physiol Vol.586(12) Pages: 2961-2973
• [Journal Article] Loss of aromatase cytochrome P450 function as a risk factor for Parkinson's disease? (2008)
  • Author(s): Morale M.C., L'Episcopo F., Tirolo C., Giaquinta G., Caniglia S., Testa N., Arcieri P., Serra P.A., Lupo G., Alberghina M., Harada N., Honda S., Panzica G.C., Marchetti B.
  • Journal Title: Brain Res. Rev. Vol.57(2) Pages: 431-443
  • Peer Reviewed
• [Journal Article] Acute effects of oestrogen on the guinea pig and human lKrchannels and drug-induced prolongation of cardiac repolarization (2008)
  • Author(s): Kurokawa, J., et al.
  • Journal Title: J. Physiol. 586 Pages: 2961-2973
  • Peer Reviewed
• [Journal Article] Loss of aromatase cytochrome P450 function as a risk factor for Parkinson's disease? (2008)
  • Author(s): Morale, M. C., et al.
  • Journal Title: Brain Res. Rev. 57 Pages: 431-443
  • Peer Reviewed
• [Journal Article] Effects of organisational oestradiol on adult immunoreactive oestrogen receptors (alpha and beta) in the male mouse brain (2007)
  • Author(s): Kudwa A.E., Harada N., Honda S., Rissman E.F.
  • Journal Title: J. Neuroendocrinol Vol.19(10) Pages: 767-772
  • Peer Reviewed
• [Journal Article] Effects of organisational oestradiol on adult immunoreactive oestrogen receptors(alpha and beta)in the male mouse brain (2007)
  • Author(s): Kudwa, A. E., et. al.
  • Journal Title: J. Neuroendocrinol. 19 Pages: 767-772
  • Peer Reviewed
• [Presentation] Analysis of functional domain of Deaf1 protein for the activation of the brain-specific aromatase gene expression (2009)
  • Author(s): 本田伸一郎
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] Analysis of functional domain of Deaf1 protein for the activation of the brain-specific aromatase gene expression (2009)
  • Author(s): 本田伸一郎
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] Multiple transcription factors regulate the brain-specific promoter activity of the aromatase gene (2008)
  • Author(s): 本田伸一郎
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Multiple transcription factors regulate the brain-specific promoter activity of the aromatase gene (2008)
  • Author(s): 本田伸一郎
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
• [Presentation] アロマターゼ脳特異的プロモーターに作用する転写因子DEAF-1 (2007)
  • Author(s): 本田伸一郎
  • Organizer: およびUbc9の作用第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] アロマターゼ脳特異的プロモーターに作用する転写因子DEAF-1、およびUbc9の作用 (2007)
  • Author(s): 本田 伸一郎
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜