Functional analysis of the novel lymphoma-associated gene, TFL, on the long arm of chromosome 6

• Project/Area Number: 19591113
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Kobe University
• Principal Investigator: YAMAMOTO Katsuya Kobe University, 大学院医学研究科, 非常勤講師 (60306199)
• Co-Investigator(Kenkyū-buntansha): MATSUI Toshimitsu 神戸大学, 大学院医学研究科, 准教授 (10219371)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: TFL遺伝子 / 遺伝子導入 / 細胞周期 / 細胞内局在 / 癌抑制遺伝子 / アポトーシス / ノックアウトマウス / FISH

Research Abstract

我々は形質転換した濾胞性リンパ腫におけるIgK遺伝子との染色体転座から、6q25に位置しリンパ球に発現する新規癌抑制遺伝子候補TFLを同定した。TFLには2つのスプライスバリアントP58TFL、P36TFLが存在し、いずれもBa/F3やJurkat細胞に過剰発現させると、Rbリン酸化の抑制を介してG1期からS期への移行が抑制された。またP58TFLは細胞質内に顆粒状に存在し、P-bodyマーカーとの共局在も認められた。CCCHタイプのジンクフィンガーを有しP-bodyと局在するP58TFLは、Rbの上流の分子の転写後調節を介して、細胞増殖の制御に重要な役割を果たすと考えられた。

Research Products

• [Journal Article] Unbalanced whole-arm translocation der(5;19) (p10;q10) is a novel and recurrent cytogenetic aberration in myelodysplastic syndrome (2009)
  • Author(s): Yamamoto K, Okamura Y, Katayama Y, Shimoyama M, Matsui T
  • Journal Title: Leukemia Research 33 Pages: 377-383
  • NAID: 120001408338
  • Peer Reviewed
• [Journal Article] Inhibition of G1 to S phase progre. ssion by a novel zinc finger protein, p58TFL at P-bodies (2009)
  • Author(s): Kentaro Minagawa
  • Journal Title: Molecular Cancer Research (印刷中)
  • Peer Reviewed
• [Journal Article] Unbalanced whole-arm translocation der(5 ; 19)(p1O ; q1O)is a novel and recurrent cytogenetic aberration in myelodysplastic syndrome (2009)
  • Author(s): Katsuya Yamamoto
  • Journal Title: Leukemia Research 第33巻・第3号 Pages: 377-383
  • Peer Reviewed
• [Journal Article] Imatinib resistance in a novel translocation der(17)t(1;17)(q25;p13) with loss of TP53 but without BCR/ABL kinase domain mutation in chronic myelogenous leukemia (2008)
  • Author(s): Yamamoto K, Yakushijin K, Nishikawa S, Minagawa K, Katayama Y, Shimoyama M, Matsui T
  • Journal Title: Cancer Genetics and Cytogenetics 183 Pages: 77-81
  • NAID: 120001408339
  • Peer Reviewed
• [Journal Article] Imatinil) resistance in a novel translocation der(l7)t(1 ; 17)(q25 ; p13)with loss of TP53 but without BCR/ABL kinase(domain mutation in chronic myelogenous leukemia (2008)
  • Author(s): Kal. suya Yamamoto
  • Journal Title: Cancer Genetics and Cytogenetics 第183巻・第2号 Pages: 77-81
  • Peer Reviewed
• [Journal Article] Deregulation of a possible tumor suppressor gene, ZC3H12D, by translocation of IGK@ in transformed follicular lymphoma with t(2;6)(p12; q25) (2007)
  • Author(s): Minagawa K, Yamamoto K, Nishikawa S, Ito M, Sada A, Yakushijin K, Okamura A, Shimoyama M, Katayama Y, Matsui T
  • Journal Title: British Journal of Haematology 139 Pages: 161-163
  • Peer Reviewed
• [Journal Article] A novel t(8;18)(q13;q21) in acute monocytic leukemia evolving from constitutional trisomy 8 mosaicism (2007)
  • Author(s): Yamamoto K, Okamura A, Kawano H, Katayama Y, Shimoyama M, Matsui T
  • Journal Title: Cancer Genetics and Cytogenetics 176 Pages: 144-149
  • NAID: 120001408351
  • Peer Reviewed
• [Journal Article] Derivative (3)t(3;18)(q27;q21)t(18;16)(q21;?) involving the BCL2 and BCL6 genes in follicular lymphoma with t(3;14;18) (q27;q32;q21) (2007)
  • Author(s): Yamamoto K, Ono K, Katayama Y, Shimoyama M, Matsui T
  • Journal Title: Cancer Genetics and Cytogenetics 179 Pages: 69-75
  • NAID: 120001408350
  • Peer Reviewed
• [Journal Article] Deregulation of a possible tumor suppressor gene, ZC3H12D, by transl-ocation of IGK@ in transformed follicular lymphoma with t(2;6)(p12;q25) (2007)
  • Author(s): Kentaro Minagawa
  • Journal Title: British Journal of Haematology 第139巻・第1号 Pages: 161-163
  • Peer Reviewed
• [Journal Article] A novel t(8;18)(q13;q21)in acute monocytic leukemia evolving from con-stitutional trisomy 8 mosaicism (2007)
  • Author(s): Katsuya Yamamoto
  • Journal Title: Cancer Genetics and Cytogenetics 第176巻・第2号 Pages: 144-149
  • NAID: 120001408351
  • Peer Reviewed
• [Journal Article] Derivative (3)t(3;18)(q27;q21) involving the BCL2 and BCL6 genes in fo-llicular lymphoma with t(3;14;18)(q27;q32;q21) (2007)
  • Author(s): Katsuya Yamamoto
  • Journal Title: Cancer Genetics and Cytogenetics 第179巻・第1号 Pages: 69-75
  • Peer Reviewed
• [Journal Article] Inhibition of G1 to S phase progression by a novel zinc finger protein
  • Author(s): Minagawa K, Katayama Y, Nishikawa S, Yamamoto K, Sada A, Okamura A, Shimoyama M, Matsui T
  • Journal Title: Molecular Cancer Research 58^<TFL> Pages: 0-0
  • Peer Reviewed
• [Presentation] 不均衡転座der(5;19)(p10; q10)はMDSにおける新たな再発性の染色体異常である (2008)
  • Author(s): 山本克也
  • Organizer: 第70回日本血液学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2008-10-12
• [Presentation] 不均衡転座der(5 ; l9)(p1O ; q1O)はMDSにおける析たなI町発性の染色体異常である (2008)
  • Author(s): 山木 克也
  • Organizer: 第70回日木血液学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2008-10-12
• [Presentation] 先天性トリソミー8モザイクより発症し、新たな転座t(8;18)(q13; q21)の出現を認めた急性単球性白血病 (2007)
  • Author(s): 山本克也
  • Organizer: 第69回日本血液学会総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-10-11
• [Presentation] 先天性トリソミー8モザイクより発症し、新たな転座t(8;18)(q13;q21)の出現を認めた急性単球性白血病 (2007)
  • Author(s): 山本 克也
  • Organizer: 第69回日本血液学会総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-10-11
• [Remarks]
  • URL: http://www.med.kobe-u.ac.ip/im3/rinsyo/ketueki/index.html
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/im3/rinsyo/ketueki/index.html