A genomic analysis of acute myeloid leukemia.

• Project/Area Number: 19591132
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Jichi Medical University
• Principal Investigator: YAMASHITA Yoshihiro Jichi Medical University, 医学部, 講師 (10326861)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 急性骨髄性白血病 / DNA マイクロアレイ / Blast Bank / 染色体構造異常 / アレイCGH / DNAメチル化 / がん遺伝子

Research Abstract

我々は効率の良いゲノミクス解析を行うことを目的として、急性骨髄性白血病(AML)症例を含む様々な白血病類縁疾患の患者骨髄より造血幹細胞のみを純化保存する大規模検体収集事業「Blast Bank」を設立した。本研究において我々はBlast Bank のAML 検体の中から、核型や治療反応性などの臨床情報が整備された99 例からなる「標準検体セット」を選び出し、これらに対して網羅的なゲノミクス解析を行い、新たながん遺伝子の同定を目指すと共に、真に治療反応性にリンクする遺伝子の同定を行った。

Research Products

• [Journal Article] Genome-wide histone methylation profile for heart failure. (2009)
  • Author(s): Kaneda, R., et al. (全10 名、第3 著者)
  • Journal Title: Genes Cells 14 Pages: 69-77
  • Peer Reviewed
• [Journal Article] KIF5B-ALK, a Novel Fusion Oncokinase Identified by an Immunohistochemistry-based Diagnostic System for ALK-positive Lung Cancer. (2009)
  • Author(s): Takeuchi, K., et al. (全14名、第9 著者)
  • Journal Title: Clin Cancer Res, 15 Pages: 3143-3149
  • Peer Reviewed
• [Journal Article] MicroRNA expression profiles of human leukemias. (2008)
  • Author(s): Takada, S., Yamashita, Y., et al. (全11 名、第2 著者)
  • Journal Title: Leukemia 22 Pages: 1274-1278
  • Peer Reviewed
• [Journal Article] A mouse model of EML4-ALK-positive lung cancer. (2008)
  • Author(s): Soda M., et al. (全12名、第9 著者)
  • Journal Title: Proc Natl Acad Sci U S A 105 Pages: 19893-19897
  • Peer Reviewed
• [Journal Article] Multiplex reverse transcription-PCR screening for EML4-ALK fusion transcripts. (2008)
  • Author(s): Takeuchi, K., et al. (全14 名、第9 著者)
  • Journal Title: Clin Cancer Res 14 Pages: 6618-6624
  • Peer Reviewed
• [Journal Article] Chromosome copy number analysis in screening for prognosis-related genomic regions. (2008)
  • Author(s): Kurashina, K., Yamashita, Y., et al. (全16 名、第2 著者)
  • Journal Title: Cancer Sci 99 Pages: 1835-1840
  • Peer Reviewed
• [Journal Article] Identification of novel isoforms of the EML4-ALK transforming gene in non-small cell lung cancer. (2008)
  • Author(s): Choi, Y. L., et al. (全18 名、第15 著者)
  • Journal Title: Cancer Res 68 Pages: 4971-4976
  • Peer Reviewed
• [Journal Article] High-resolution analysis of chromosome copy number alterations in angioimmunoblastic T-cell lymphoma and peripheral T-cell lymphoma, unspecified, with single nucleotide polymorphism-typing microarrays. (2008)
  • Author(s): Fujiwara, S. I., Yamashita, Y., et al. (全14 名、第2 著者)
  • Journal Title: Leukemia 22 Pages: 1891-1898
  • Peer Reviewed
• [Journal Article] MicroRNA expression profiles of human leukemias. (2008)
  • Author(s): Takada, S., et al.
  • Journal Title: Leukemia 22 Pages: 1274-1278
  • Peer Reviewed
• [Journal Article] High-resolution analysis of chromosome copy number alterations in angioimmunoblastic T-cell lymphoma and peripheral T-cell lymphoma, unspe cified, with single nucleotide polymorphism-typing microarrays. (2008)
  • Author(s): Fujiwara, S. I., et al.
  • Journal Title: Leukemia 22 Pages: 1891-1898
  • Peer Reviewed
• [Journal Article] A mouse model for EML4-ALK-positive lung cancer. (2008)
  • Author(s): Soda, M., et al.
  • Journal Title: Proc Natl Acad Sci U S A 105 Pages: 19893-19897
  • Peer Reviewed
• [Journal Article] Analysis of chromosome copy number in leukemic cells by different microarray platforms. (2007)
  • Author(s): Yamashita, Y., et al. (全12 名、筆頭著者)
  • Journal Title: Leukemia 21 Pages: 1333-1337
  • Peer Reviewed
• [Journal Article] Transforming activity of purinergic receptor P2Y, G protein coupled, 8revealed by retroviral expression screening. (2007)
  • Author(s): Fujiwara, S., Yamashita, Y., et al. (全11 名、第2 著者)
  • Journal Title: Leuk Lymphoma 48 Pages: 978-986
  • Peer Reviewed
• [Journal Article] Identification of the transforming EML4-ALK fusion gene in non-mall-cell lung cancer. (2007)
  • Author(s): Soda, M., et al. (全18名、第5 著者)
  • Journal Title: Nature 448 Pages: 561-566
  • Peer Reviewed
• [Journal Article] Identification of a constitutively active mutant of JAK3 by retroviral expression screening. (2007)
  • Author(s): Choi, Y. L., et al. (全12 名、第11 著者)
  • Journal Title: Leuk Res 31 Pages: 203-209
  • Peer Reviewed
• [Journal Article] Transforming activity of purinergic receptor P2Y, G-protein coupled, 2revealed by retroviral expression screening. (2007)
  • Author(s): Hatanaka, H., et al. (全11 名、第9 著者)
  • Journal Title: Biochem Biophys Res Commun 356 Pages: 723-726
  • Peer Reviewed
• [Journal Article] A genomic analysis of adult T-cell leukemia. (2007)
  • Author(s): Choi, Y. L., et al. (全16 名、第8 著者)
  • Journal Title: Oncogene 26 Pages: 1245-1255
  • Peer Reviewed
• [Journal Article] Analysis of chromosome copy number in leukemic cells by different microarray platforms. (2007)
  • Author(s): Yamashita Y., et. al.
  • Journal Title: Leukemia 21 Pages: 1333-1337
  • Peer Reviewed
• [Journal Article] Transforming activity of purinergic receptor P2Y,G protein coupled,8 revealed by retroviral expression screening. (2007)
  • Author(s): Fujiwara S., et. al.
  • Journal Title: Leuk..Lymphoma 48 Pages: 978-986
  • Peer Reviewed
• [Journal Article] Identification of the transforming EML4-ALK fusion gene in non-small-cell lung cancer (2007)
  • Author(s): Soda M., et. al.
  • Journal Title: Nature 448 Pages: 561-566
  • Peer Reviewed
• [Presentation] A large-scale resequencing project of ~5600 human genes in the genome of acute myeloid leukemia. (2009)
  • Author(s): Yamashita, Y., Soda, M., Mano, H.
  • Organizer: AACR 100th Annual Meeting 2009
  • Place of Presentation: Denver, U.S.A.
• [Presentation] 大型リ・シークエンスアレイによる白血病の遺伝子変異スクリーニング (2008)
  • Author(s): 山下 義博
  • Organizer: 第70回日本血液学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-10-11
• [Presentation] A large-scale resequencing Project of 〜5400 human genes in the genome of acute myeloid leukemia. (2008)
  • Author(s): Yamashita Y., et. al.
  • Organizer: Keystone Symposia
  • Place of Presentation: 米国コロラド州
  • Year and Date: 2008-03-27
• [Presentation] 大型リ・シークエンスアレイによる白血病の遺伝子変異スクリーニング (2008)
  • Author(s): 山下義博, 崔永林, 高田修治, 間野博行
  • Organizer: 第70 回日本血液学会総会
  • Place of Presentation: 京都
• [Presentation] A large-scale resequencing project of ~5400 human genes in the genome of acute myeloid leukemia. (2008)
  • Author(s): Yamashita, Y., Kurashina, K., Soda, M., Watanabe, H., Mano, H.
  • Organizer: Signaling Pathways in Cancer and Development
  • Place of Presentation: Steamboat Springs, U.S.A.
• [Presentation] Analysis of chromosome copy number in leukemic cells by different microarray platforms. (2007)
  • Author(s): Yamashita, Y., Kurashina, K., Watanabe, H., Soda, M., Hatanaka, H., Mano, H.
  • Organizer: Molecular Targets for Cancer
  • Place of Presentation: Whistler, Canada