| Project/Area Number |
19591194
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | Sagami Women's Junior College |
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Principal Investigator |
SEIKE Masahiro Sagami Women's Junior College, 短期大学部・食物栄養学科, 教授 (50346714)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | ヒスタミン / 知覚神経 / 皮膚炎 / H4受容体 / アレルギー性皮膚炎 / 肥満細胞 / 好酸球 / 慢性アレルギー性皮膚炎 / 慢性アレルギー性接触皮膚炎 / Th1 / Th2 / サイトカイン / 皮膚アレルギー反応 / 接触皮膚炎 / ヒスタミン第4受容体 / IgE |
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| Research Abstract |
Background : The present study observed effects of the histamine H4 receptor on chronic allergic contact dermatitis induced by repeated challenge in mice.
Methods : Acute contact dermatitis was induced by single epicutaneous challenge of 2,4,6-trinitro-1-chlorobenzene (TNCB) to the ear. Chronic allergic contact dermatitis was developed by repeated epicutaneous challenge using TNCB on the dorsal back skin. H4 receptor antagonist JNJ7777120 was administered to wild-type mice, while H4 receptor agonist 4-methylhistamine was administered to histidine decarboxylase (HDC) (-/-) mice that synthesized no histamine.
Results : HDC (-/-) mice did not differ phenotypically from HDC (+/+) mice and H4 receptor antagonist/agonist did not have clinical effects in terms of acute contact dermatitis reactions. H4 receptor antagonist ameliorated skin eczematous lesions induced by repeated TNCB challenge in HDC (+/+) mice. On the contrary, H4 receptor agonist exacerbated skin lesions exclusively in HDC (-/-) mice. Application of H4 receptor agonist induced migration of mast cells and eosinophils in skin lesions and H4 receptor antagonist suppressed these changes. H4 receptor was immunohistochemically detected on mast cells in eczematous lesions. Levels of interleukin (IL)-4, -5, and -6 in lesions were decreased, whereas levels of interferon-γ and IL-12 were increased by H4 receptor antagonistic activity. Serum Immunoglogulin E levels rapidly increased with repeated challenge, but decreased with H4 receptor antagonist.
Conclusion : Since chronic allergic contact dermatitis is developed by H4 receptor stimulation, H4 receptor antagonists might represent new candidate drugs for treating chronic allergic contact dermatitis.
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