The effect of calcitropic hormone on bone forming action of・-cateninsignal

• Project/Area Number: 19591204
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Osaka University
• Principal Investigator: OZONO Keiichi Osaka University, 医学系研究科, 教授 (20270770)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: カルシウム調節ホルモン / wntシグナル / 骨量 / 骨粗鬆症 / 骨形成 / Wntシグナル

Research Abstract

細胞株を用いて、活性型ビタミンD、副甲状腺ホルモン(PTH)などのカルシウム調節ホルモンとcanonical wnt経路のクロストークが存在することを証明した。さらに、wntのcoreceptorの1つであるlow-density lipoprotein receptor-related protein 6(Lrp6)の骨代謝における機能をLRP6の機能が低下した自然発症マウスrsを用い、解析した。その結果、wntのLRP6を介する効果としては骨芽細胞におけるRANKLの発現を抑制する事によって破骨細胞形成を抑制し、骨量を増加させることである事を証明し、論文として公表した。

Research Products

• [Journal Article] Causes and Differential Diagnosis of Hypocalcemia - Recommendation Proposed by Expert Panel Supported by Ministry of Health (2009)
  • Author(s): Fukumoto S, Namba N, Ozono K,(他8名)
  • Journal Title: Labour and Welfare.Japan -Endocr J (in press)
  • Peer Reviewed
• [Journal Article] Severe arterial hypertension : a possible complication of McCune-Albright syndrome (2009)
  • Author(s): Ohata Y, Yamamoto T, Ozono K(9番目),(他6名)
  • Journal Title: Eur J Pediate (in press)
  • Peer Reviewed
• [Journal Article] Causes and Differential Diagnosis of Hypocalcemia-Recommendation Proposed by Expert Panel Supported by Ministry of Health, Labour and Welfare. (2009)
  • Author(s): Fukumoto S
  • Journal Title: Japan-Endocr J. in press
  • Peer Reviewed
• [Journal Article] Severe arterial hypertension : a possible complication of McCune-Albright syndrome. (2009)
  • Author(s): Ohata Y
  • Journal Title: Eur J Pediate. in press
  • Peer Reviewed
• [Journal Article] Clinical usefulness of measurement of fibroblast growth factor 23 (FGF23) in hypophosphatemic patients : proposal of diagnostic criteria using FGF23 measurement (2008)
  • Author(s): Endo I, Fukumoto S, Ozono K,(他8名)
  • Journal Title: Bone 42(6) Pages: 1235-1239
  • Peer Reviewed
• [Journal Article] PTH/cAMP/PKA Signaling Facilitates Canonical Wnt Signaling Via Inactivation of Glycogen Synthase Kinase-3βin Osteoblastic saos-2 Cells.Journal of Cellular (2008)
  • Author(s): Suzuki A, Ozono K, Kubota T, (他3名)
  • Journal Title: Biochemistry 104(1) Pages: 304-317
  • Peer Reviewed
• [Journal Article] Lrp6 hypomorphic mutation affects bone mass through bone resorption in mice and impairs interaction with Mesd (2008)
  • Author(s): Kubota T, Michigami T, Ozono K(10番目),(他7名)
  • Journal Title: J Bone Miner Res 23(10) Pages: 1661-1671
  • Peer Reviewed
• [Journal Article] Severe mandibuloacral dysplasia caused by novel compound heterozygous ZMPSTE24 mutations in two Japanese siblings (2008)
  • Author(s): Miyoshi Y, Akagi M, Ozono K(14番目),(他11名)
  • Journal Title: Clin Genet 73(6) Pages: 535-544
  • Peer Reviewed
• [Journal Article] The molecular mechanism underlying Roberts syndrome involves loss of ESCO2 acetyltransferase activity (2008)
  • Author(s): Gordillo M, Vega H, Ozono K(23番目)(他23名)
  • Journal Title: Hum Mol Genet 17(14) Pages: 2172-2180
  • Peer Reviewed
• [Journal Article] 低リン血症性疾患の病態と治療-クル病/骨軟化症を含めて- (2008)
  • Author(s): 大薗恵一
  • Journal Title: THE BONE 22(6) Pages: 747-751
• [Journal Article] PTH/cAMP/PKA Signaling Facilitates Canonical Wnt Signaling Via Inactivation of Glycogen Synthase Kinase-3βin Osteoblastic saos-2 Cells. (2008)
  • Author(s): Suzuki A
  • Journal Title: Journal of Cellular Biochemistry 104 Pages: 304-317
  • Peer Reviewed
• [Journal Article] Lrp6 hypomorphic mutation affects bone mass through bone resorption in mice and impairs interaction with Mesd. (2008)
  • Author(s): Kubota T
  • Journal Title: J Bone Miner Res. 23(10) Pages: 1661-1671
  • Peer Reviewed
• [Journal Article] Clinical usefulness of measurement of fibroblast growth factor 23 (FGF23)in hypophosphatemic patients : proposal of diagnostic criteria using FGF23 measurement. (2008)
  • Author(s): Endo I
  • Journal Title: Bone. 42(6) Pages: 1235-1239
  • Peer Reviewed
• [Journal Article] Severe mandibuloacral dysplasia caused by novel compound heterozygous ZMPSTE24mutations in two Japanese siblings. (2008)
  • Author(s): Miyoshi Y
  • Journal Title: Clin Genet. 73(6) Pages: 535-544
  • Peer Reviewed
• [Journal Article] The molecular mechanism underlying Roberts syndrome involves loss of ESC02 acetyltransferase activity. (2008)
  • Author(s): Gordillo M
  • Journal Title: Hum Mol Genet. 17(14) Pages: 2172-2180
  • Peer Reviewed
• [Journal Article] 低リン血症性疾患の病態と治療-クル病/骨軟化症を含めて- (2008)
  • Author(s): 大薗 恵一
  • Journal Title: THE BONE 22(6) Pages: 747-751
• [Journal Article] PTH/cAMP/PKA signaling facilitates canonical Wnt signaling via inactivation of glycogen synthase kinase-3beta in osteoblastic Saos-2 cells. (2007)
  • Author(s): Suzuki A
  • Journal Title: J Cell Biochem. Nov7(Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Wnt/Lrp/beta-catenin signaling suppresses adipogenesis by inhibiting mutual activation of PPARgammaand C/EBPalpha. (2007)
  • Author(s): Kawai M
  • Journal Title: Biochem Biophys Res Commun. 363(2) Pages: 276-282
  • Peer Reviewed
• [Journal Article] Involvement of nuclear factor I transcription/replication factor in the early stage of chondrocytic differentiation. (2007)
  • Author(s): Uchihashi T
  • Journal Title: Bone. 41(6) Pages: 1025-1035
  • Peer Reviewed
• [Presentation] 骨量を規定する新たな遺伝子LRP6に変異を有するマウスの解析 (2008)
  • Author(s): 大薗恵一
  • Organizer: 第42回日本小児内分泌学会学術集
  • Place of Presentation: 鳥取
• [Presentation] 血清カルシウム(Ca)・リン(P)値の異常とその原因 (2007)
  • Author(s): 大薗恵一
  • Organizer: 第173回大阪小児科学会
  • Place of Presentation: 大阪市
  • Year and Date: 2007-04-07
• [Book] 今日の治療指針-私はこう治療している-くる病 (2008)
  • Author(s): 大薗恵一
  • Publisher: 医学書院
• [Book] 小児科学第3版8章先天代謝異常症, 代謝疾患Jくる病Rickets (2008)
  • Author(s): 大薗恵一
  • Publisher: 医学書院
• [Book] 今日の治療指針-私はこう治療している- (2008)
  • Author(s): 大薗 恵一
  • Publisher: 医学書院
• [Book] 小児科学第3版 8章 先天代謝異常症, 代謝疾患 J くる病 Rickets (2008)
  • Author(s): 大薗 恵一
  • Publisher: 医学書院
• [Book] 小児科診療ガイドライン-最新の診療指針- (2007)
  • Author(s): 大薗 恵一
  • Publisher: 総合医学社