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Pathophysiological analysis of human bocavirus infection and separation of virus particle

Research Project

Project/Area Number 19591235
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionHokkaido University

Principal Investigator

ISHIGURO Nobuhisa  Hokkaido University, 病院, 助教 (40168216)

Co-Investigator(Kenkyū-buntansha) ENDO Rika  北海道大学, 大学院・医学研究科, 助教 (30399852)
ARIGA Tadashi  北海道大学, 大学院・医学研究科, 教授 (60322806)
KASAHARA Masanori  北海道大学, 大学院・医学研究科, 教授 (30241318)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords小児感染症学 / ヒトボカウイルス感染症 / 抗体 / ヒトボカウイルス
Research Abstract

ヒトボカウイルス(Human bocavirus ; HBoV)は2005年にスウェーデンの研究者により発見されたウイルスである。まず最初に本研究ではバキュロウイルス・昆虫細胞発現系を用いた抗HBoV IgG抗体の測定方法を確立した。4名の呼吸器感染症の小児患者の回復期血清中の抗HBoV IgG抗体の上昇を認めるとともに、鼻咽頭ぬぐい液と血清からHBoVゲノムを検出した。年齢層別の抗HBoV IgG抗体陽性率をみると、6-8か月の抗体陽性率は最低であるが、その後上昇して6歳以降のほとんど全ての人が抗HBoV抗体を保有していることがわかった。これらのことから、HBoVは呼吸器感染症に関連する重要なウイルスであると推定された。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (25 results)

All 2010 2009 2008 2007

All Journal Article (20 results) (of which Peer Reviewed: 13 results) Presentation (5 results)

  • [Journal Article] 【今後留意すべき感染症】ヒトボカウイルス感染症2010

    • Author(s)
      石黒信久, 遠藤理香
    • Journal Title

      化学療法の領域 26巻、2号

      Pages: 250-257

    • Related Report
      2008 Final Research Report
  • [Journal Article] Antibodies against structural and nonstructural proteins of human bocavirus in human sera2010

    • Author(s)
      Shirkoohi, R., R. Endo, N. Ishiguro, S. Teramoto, H. Kikuta, T. Ariga
    • Journal Title

      Clin Vaccine Immunol 17(1)

      Pages: 190-3

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antibodies against structural and nonstructural proteins of human bocavirus in human sera2010

    • Author(s)
      Shirkoohi, R, et al.
    • Journal Title

      Clin Vaccine Immunol 17

      Pages: 190-3

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 【今後留意すべき感染症】ヒトボカウイルス感染症2010

    • Author(s)
      石黒信久、遠藤理香
    • Journal Title

      化学療法の領域 26

      Pages: 250-7

    • Related Report
      2008 Annual Research Report
  • [Journal Article] ヒトボカウィルス感染症2009

    • Author(s)
      石黒信久, 遠藤理香
    • Journal Title

      臨床検査 53巻、1号

      Pages: 97-104

    • Related Report
      2008 Final Research Report
  • [Journal Article] 【最近注日される微生物 その臨床的意義と検査法 (ウィルス)】注目されるウイルス ヒトボカウイルス2009

    • Author(s)
      石黒信久, 遠藤理香, 菊田英明
    • Journal Title

      臨床と微生物 36、3号

      Pages: 199-204

    • Related Report
      2008 Final Research Report
  • [Journal Article] 【最前線! ICTが注意すべき微生物対策10】RSウイルス、ヒトメタニューモウィルス、ボカウィルス2009

    • Author(s)
      石黒信久
    • Journal Title

      INFECTION CONTROL 18巻、10号

      Pages: 1007-1011

    • Related Report
      2008 Final Research Report
  • [Journal Article] Clinical and microbiological impact of human bocavirus on children with acute otitis media2009

    • Author(s)
      L. B. Beder, M. Hotomi, M. Ogami, K. Yamauchi, J. Shimada, D. S. Billal, N. Ishiguro, N. Yamanaka
    • Journal Title

      Eur J Pediatr 168(11)

      Pages: 1365-72

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human bocavirus DNA detected in a boy with plastic bronchitis2009

    • Author(s)
      Oikawa, J., J. Ogita, N. Ishiwada, T. Okada, R. Endo, N. Ishiguro, K. Ubukata, Y. Kohno
    • Journal Title

      Pediatr Infect Dis J 28(11)

      Pages: 1035-6

    • NAID

      120005341799

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of a lateral-flow immunochromatography assay with real-time reverse transcription-PCR for detection of human metapneumovirus.2008

    • Author(s)
      Kikuta, H., C. Sakata, R. Gamo, A. Ishizaka, Y. Koga, M. Konno, Y. Ogasawara, H. Sawada, Y. Taguchi, Y. Takahashi, K. Yasuda, N. Ishiguro, A. Hayashi, H. Ishiko, K. Kobayashi
    • Journal Title

      J Clin Microbiol 46

      Pages: 928-32

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficient multiplication of human metapneumovirus in Vero cells expressing the transmembrane serine protease TMPRSS22008

    • Author(s)
      Shirogane, Y., M. Takeda, M. Iwasaki, N. Ishiguro, H. Takeuchi, Y. Nakatsu, M. Tahara, H. Kikuta, Y. Yanagi
    • Journal Title

      J Virol 82(17)

      Pages: 8942-6

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of four genetic subgroup-specific antibodies to human metapneumovirus attachment (G) protein in human serum2008

    • Author(s)
      Endo, R., T. Ebihara, N. Ishiguro, S. Teramoto, T. Ariga, C. Sakata, A. Hayashi, H. Ishiko, H. Kikuta
    • Journal Title

      J Gen Virol 89(Pt 8)

      Pages: 1970-7

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two novel NKG2D ligands of the mouse H60 family with differential expression patterns and binding affinities to NKG2D2008

    • Author(s)
      Miyatake, U. Tomaru, M. Sakai, H. Chiba, K. Maenaka, D. Kohda, K. Fugo, M. Kasahara
    • Journal Title

      J Immunol 180(3)

      Pages: 1678-85

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of four genetic subgroup-specific antibodies to human metapneumovirus attachment(G)protein in human serum2008

    • Author(s)
      Endo, R., et al.
    • Journal Title

      J Gen Virol 89

      Pages: 1970-7

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of four genetic subgroup-specific antibodies to human metapneumovirus attachment (G) protein in human serum2008

    • Author(s)
      Endo, R., et al.
    • Journal Title

      J Gen Virol 89

      Pages: 1970-7

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seroepidemiology of human bocavirus in Hokkaido prefecture, Japan2007

    • Author(s)
      Endo, R., N. Ishiguro, H. Kikuta, S. Teramoto, R. Shirkoohi, X. Ma, T. Ebihara, H. Ishiko, T. Ariga
    • Journal Title

      J Clin Microbiol 45

      Pages: 3218-23

    • NAID

      120000955807

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a rapid chromato-graphic immunoassay for detection of human metapneumovirus using monoclonal antibodies against nucleoprotein of hMPV2007

    • Author(s)
      Kikuta, H., T. Ebihara, R. Endo, N. Ishiguro, C. Sakata, S. Ochiai, H. Ishiko, R. Gamo, T. Sato
    • Journal Title

      Hybridoma (Larchmt) 26

      Pages: 17-21

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 日本におけるヒトボカウイルス感染症の臨床像2007

    • Author(s)
      遠藤理香, 石黒信久
    • Journal Title

      小児科 48巻、6号

      Pages: 957-963

    • NAID

      40015487420

    • Related Report
      2008 Final Research Report
  • [Journal Article] 話題の感染症ヒトボカウイルス感染症2007

    • Author(s)
      石黒信久, 遠藤理香, 有賀正
    • Journal Title

      Modern Media 53巻、10号

      Pages: 259-266

    • Related Report
      2008 Final Research Report
  • [Journal Article] Seroepidemiology of human bocavirus in hokkaidou prefecture, Japan2007

    • Author(s)
      Endo, R., et al.
    • Journal Title

      J Clin Microbiol 45

      Pages: 3218-3223

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 感染症update18「ヒトボカウイルス感染症」2009

    • Author(s)
      石黒信久
    • Organizer
      第83回日本感染症学会総会・学術講演会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] 進歩するウィルス検査の現状と将来展望2009

    • Author(s)
      石黒信久
    • Organizer
      第20回日本臨床微生物学会総会シンポジウム
    • Place of Presentation
      仙台
    • Related Report
      2008 Final Research Report
  • [Presentation] 感染症 update18「ヒトボカウイルス感染症」2009

    • Author(s)
      石黒信久
    • Organizer
      第83回日本感染症学会総会・学術講演会
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report
  • [Presentation] 最近の小児呼吸器ウイルスの話題-ヒトメタニューモウィルスとヒトボカウイルスを中心に-2008

    • Author(s)
      石黒信久
    • Organizer
      第5回神奈川小児血液・感染症フォーラム (横浜) 特別講演
    • Place of Presentation
      金
    • Year and Date
      2008-09-05
    • Related Report
      2008 Final Research Report
  • [Presentation] ヒトボカウィルス (HBoV) 感染症における血清中のウィルスゲノムの検出と抗HBoVIgG抗体価の推移2007

    • Author(s)
      石黒信久
    • Organizer
      第39回日本小児感染症学会総会
    • Place of Presentation
      横浜(特別講演)
    • Related Report
      2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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