Establishment of mesenchymal stem cells from cryopreserved-cord blood for treatment of acute graft-versus-host disease
| Project/Area Number |
19591237
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|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TSUCHIYA Tsuchiya 東北大学, 大学院・医学系研究科, 教授 (30124605)
|
|---|
| Project Period (FY) |
2007 – 2008
|
| Project Status |
Completed (Fiscal Year 2008)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 臍帯血 / 間葉系幹細胞 / 移植片対宿主病 |
|---|
| Research Abstract |
研究当初に目標とした大豆凝集素による濃縮操作後に樹立した臍帯血由来間葉系幹細胞培養を長期に維持することが困難であった。また液体窒素中に凍結保存された臍帯血を解凍後に分離培養した単核球からは間葉系幹細胞は樹立されなかった。以後は採取から培養開始までの時間の短い新鮮な臍帯血を実験に供することとし、間葉系幹細胞培養用にスクリーニングされた胎児牛血清を添加した間葉系幹細胞培養用培地を用いることで増幅可能な2株の間葉系幹細胞を得ることができた
|
Report
(3 results)
Research Products
(3 results)
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[Journal Article] Quality evaluation of umbilical cord blood progenitor cells cryopreserved with a small-scale automated liquid nitrogen system2008
Author(s)
Miura J, Minegishi M, Itoh T, Kitaura T, Fukawa N, Takahashi H, Suzuki A, Kudo Y, Narita A, Sato Y, Suzuki M, Wada Y, Takeyama Y, Watanabe T, Tsuchiya S
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Journal Title
Cryobiology 57
Pages: 178-181
Related Report
Peer Reviewed
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[Journal Article] Quality of umbilical cord blood CD34+ cells in a double- compartment freezing bag cryopreserved without a rate- controlled programmed freezer2007
Author(s)
Minegishi M, Itoh T, Fukawa N, Kitaura T, Miura J, Takahashi H, Suzuki A, Kudo Y, Narita A, Sato Y, Suzuki M, Watanabe T, Wada Y, Takeyama Y, Tsuchiya S
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Journal Title
Int J Hematol 85
Pages: 78-84
Related Report
Peer Reviewed
