Isolation of neuroblastoma stem cell to compare with neural stem cell

• Project/Area Number: 19591261
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Nagoya City University
• Principal Investigator: AOYAMA Mineyoshi Nagoya City University, 大学院・医学研究科, 講師 (70363918)
• Co-Investigator(Kenkyū-buntansha): ASAI Kiyofumi 名古屋立大学, 大学院・医学研究科, 教授 (70212462)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 神経芽細胞腫 / 腫瘍幹細胞 / 神経幹細胞 / マイクロアレイ

Research Abstract

We had a plan to isolate putative cancer stem cells from mouse neuroblastoma cell lines. And also we would isolate mouse neural stem cells from mouse embryonal and adult brain. Following the isolation of both stem cells, we would find the similarity and the difference between neuroblastoma cancer stem cells and neural stem cells. We have isolated mouse neural stem cells. However, we have failed to isolate neuroblastoma cancer stem cells using the sorting method by the specific cell surface marker or the neurosphere method. We suggest that after frequent passages, cancer cell lines could lose the cancer stem cells, which can be found in human primary tumor.

Research Products

• [Journal Article] Prolonged exposure to ammonia increases extracellular glutamate in cultured rat astrocytes. (2009)
  • Author(s): Ohara, K., Aoyama, M., Fujita, M., Sobue, K., Asai, K.
  • Journal Title: Neurosci Lett Pages: 109-112
  • Peer Reviewed
• [Journal Article] Diclofenac enhances proinflammatory cytokine-induced nitric oxide production through NF-kappaB signaling in cultured astrocytes. (2009)
  • Author(s): Kakita, H., Aoyama, M., Hussein, M.H., Kato, S., Suzuki, S., Ito, T., Togari, H., Asai, K.
  • Journal Title: Toxicol Appl Pharmacol Pages: 56-63
  • Peer Reviewed
• [Journal Article] Silicone-induced foreign-body reaction after first metatarsophalangeal jointarthroplasty for Jaccoud's arthropathy. (2009)
  • Author(s): Waguri-Nagaya, Y., Yamagami, T., Tsuchiya, A., Nozaki, M., Goto, H., Kobayashi, M., Aoyama, M., Tada, T., Otsuka, T.
  • Journal Title: Rheumatol Int Pages: 1367-1371
  • Peer Reviewed
• [Journal Article] IGF2 modulates the microenvironment for osteoclastogenesis. (2009)
  • Author(s): Nakao, K., Aoyama, M., Fukuoka, H., Fujita, M., Miyazawa, K., Asai, K., Goto, S.
  • Journal Title: Biochem Biophys Res Commun 378 Pages: 462-466
  • Peer Reviewed
• [Journal Article] Diclofenac enhances proinflammatory cytokine-induced nitric oxide production through NF-kappaB signaling in cultured astrocytes (2009)
  • Author(s): Kakita, H
  • Journal Title: Toxicol Appl Pharmacol 238 Pages: 56-63
  • Peer Reviewed
• [Journal Article] Prolonged exposure to ammonia increases extracellular glutamate in cultured rat astrocytes (2009)
  • Author(s): Ohara, K.
  • Journal Title: Neurosci Lett 462 Pages: 109-112
  • Peer Reviewed
• [Journal Article] Silicone-induced foreign-body reaction after first metatarsophalangeal jointarthroplasty for Jaccoud's arthropathy (2009)
  • Author(s): Waguri-Nagaya Y.
  • Journal Title: Rheumatol Int 29 Pages: 1367-1371
  • Peer Reviewed
• [Journal Article] Diclofenac enhances proinflammatory cytokine-induced nitric oxide productionthrough NF-КB signaling in cultured astrocytes. (2009)
  • Author(s): Kakita, H.
  • Journal Title: Toxicology and Applied Pharmacology in press
  • Peer Reviewed
• [Journal Article] Silicone-induced foreign-body reaction after first metatarsophalangeal jointarthroplasty for Jaccoud's arthropathy. (2009)
  • Author(s): Waguri-Nagaya Y.
  • Journal Title: Rheumatol Int in press
  • Peer Reviewed
• [Journal Article] IGF2 modulates the microenvironment for osteoclastogenesis. (2009)
  • Author(s): Nakao, K.
  • Journal Title: Biochem Biophys Res Commun 378 Pages: 462-466
  • Peer Reviewed
• [Journal Article] Severity of virilization of external genitalia in Japanese patients with salt-wasting 21-hydroxylase deficiency. (2008)
  • Author(s): Sugiyama, Y., Mizuno, H., Hayashi, Y., Imamine, H., Ito, T., Kato, I., Yamamoto-Tomita, M., Aoyama, M., Asai, K., Togari, H.
  • Journal Title: Tohoku J Exp Med Pages: 341-348
  • NAID: 10021951345
  • Peer Reviewed
• [Journal Article] Lactic acid increases aquaporin 4 expression on the cell membrane of cultured rat astrocytes. (2008)
  • Author(s): Morishima, T., Aoyama, M., Iida, Y., Yamamoto, N., Hirate, H., Arima, H., Fujita, Y., Sasano, H., Tsuda, T., Katsuya, H., Asai, K., Sobue, K.
  • Journal Title: Neurosci Res 61 Pages: 18-26
  • Peer Reviewed
• [Journal Article] The type 1 interleukin 1 receptor is not required for the death of murine hippocampal dentate granule cells and microglia activation. (2008)
  • Author(s): Harry, G.J., Funk, J.A., Lefebvre d'Hellencourt, C., McPherson, C.A., Aoyama, M.
  • Journal Title: Brain Res 1194 Pages: 8-20
  • Peer Reviewed
• [Journal Article] Tumor necrosis factor p55 and p75 receptors are involved in chemical-induced apoptosis of dentate granule neurons. (2008)
  • Author(s): Harry, G.J., d'Hellencourt, C.L., McPherson, C.A., Funk, J.A., Aoyama, M., Wine, R.N.
  • Journal Title: J Neurochem Pages: 281-298
  • Peer Reviewed
• [Journal Article] The type 1 interleukin 1 receptor is not required for the death of murine hippocampal dentate granule cells and microglia activation. (2008)
  • Author(s): Harry, G. J.
  • Journal Title: Brain Res 1194 Pages: 8-20
  • Peer Reviewed
• [Journal Article] Tumor necrosis factor p55 and p75 receptors are involved in chemical-induced apoptosis of dentate granule neurons. (2008)
  • Author(s): Harry, G. J.
  • Journal Title: J Neurochem 106 Pages: 281-298
  • Peer Reviewed
• [Journal Article] Lactic acid increases aquaporin 4 expression on the cell membrane of cultured rat astrocytes. (2008)
  • Author(s): Morishima, T.
  • Journal Title: Neurosci Res 61 Pages: 18-26
  • Peer Reviewed
• [Journal Article] Severity of virilization of external genitalia in Japanese patients with salt-wasting 21-hydroxylase deficiency. (2008)
  • Author(s): Sugiyama, Y.
  • Journal Title: Tohoku J Exp Med 215 Pages: 341-348
  • Peer Reviewed
• [Journal Article] Tumor necrosis factor p55 and p75 receptors are involved in chemical-induced apoptosis of dentate granule neurons. (2008)
  • Author(s): Harry, G. J
  • Journal Title: J Neurochem (in press)
  • Peer Reviewed
• [Journal Article] Lactic acid increases aquaporin 4 expression on the cell membrane of cultured rat astrocytes. (2008)
  • Author(s): Morishima, T.
  • Journal Title: Neurosci Res (in press)
  • Peer Reviewed
• [Journal Article] The type 1 interleukin 1 receptor is not required for the death of murine hippocampal dentate granule cells and microglia activation. (2008)
  • Author(s): Harry, G. J
  • Journal Title: Brain Res 1194 Pages: 8-20
  • Peer Reviewed
• [Journal Article] Tumor Necrosis Factor Receptor in Trimethyltin-induced Dentate Granule Cell Death. (2007)
  • Author(s): Harry, G.J., Lefebvre, d'Hellencourt. C., Wine, R., Aoyama, M.
  • Journal Title: Medimond International Proceedings Pages: 303-308
  • Peer Reviewed
• [Journal Article] Gliostatin/thymidine phosphorylase-regulated vascular endothelial growth-factor production in human fibroblast-like synoviocytes. (2007)
  • Author(s): Tanikawa, T., Waguri-Nagaya, Y., Kusabe, T., Aoyama, M., Asai, K., Otsuka, T.
  • Journal Title: Rheumatol Int 27 Pages: 553-559
  • Peer Reviewed
• [Presentation] ジクロフェナックナトリウムがアストロサイトのNO産生に及ぼす影響-インフルエンザ感染に伴う脳症悪化のメカニズム-. (2009)
  • Author(s): 青山峰芳, 垣田博樹, 加藤晋, 戸苅創, 浅井清文
  • Organizer: 第14回グリア研究会
  • Place of Presentation: 大阪
  • Year and Date: 2009-11-14
• [Presentation] ジクロフェナックナトリウムがアストロサイトのNO産生に及ぼす影響-インフルエンザ感染に伴う脳症悪化のメカニズム- (2009)
  • Author(s): 青山峰芳
  • Organizer: 第14回グリア研究会
  • Place of Presentation: 大阪
  • Year and Date: 2009-11-14
• [Presentation] TNF-αは線維芽腫細胞様滑膜細胞においてアクアポリン9の発現を誘導する. (2009)
  • Author(s): 長原正静, 永谷祐子, 山上貴也, 青山峰芳, 多田豊曠, 浅井清文, 大塚隆信
  • Organizer: 第24回日本整形科学学会基礎学術集会2009
  • Place of Presentation: 横浜
• [Presentation] ジクロフェナックがサイトカイン刺激によってアストロサイトから誘導されるNO産生を増強する-インフルエンザ脳症増悪化メカニズムの解析-. (2009)
  • Author(s): 青山峰芳, 垣田博樹, フセインモハメド, 加藤晋, 鈴木悟, 伊藤哲也, 戸苅創, 浅井清文
  • Organizer: 第52回日本神経化学会(伊香保)大会
  • Place of Presentation: 伊香保
• [Presentation] 炎症性サイトカインとCOX阻害剤がアストロサイトのNOx産生に及ぼす影響. (2009)
  • Author(s): 垣田博樹, 青山峰芳, 加藤晋, 鈴木悟, 戸苅創, 浅井清文
  • Organizer: 第112回日本小児科学会学術集会
  • Place of Presentation: 奈良
• [Presentation] Differential inflammatory gene expression in young and middle aged mice during injury-induced hippocampal neurogenesis. (2009)
  • Author(s): McPherson C.A., Aoyama M., Grissom S.F., Gohlke J., Harry G.J.
  • Organizer: The American Society for Neurochemistry 40th Annual Meeting
  • Place of Presentation: Charleston, SC, USA
• [Presentation] 炎症性サイトカインとCOX阻害剤がアストロサイトのNOx産生に及ぼす影響 (2009)
  • Author(s): 垣田博樹
  • Organizer: 第112回日本小児科学会学術集会
  • Place of Presentation: 奈良
• [Presentation] ジクロフェナックがサイトカイン刺激によってアストロサイトから誘導されるNO産生を増強する-インフルエンザ脳症増悪化メカニズムの解析- (2009)
  • Author(s): 青山峰芳
  • Organizer: 第52回日本神経化学会(伊香保)大会
  • Place of Presentation: 伊香保
• [Presentation] TNF-αは線維芽腫細胞様滑膜細胞においてアクアポリン9の発現を誘導する (2009)
  • Author(s): 長原正静
  • Organizer: 第24回日本整形科学学会基礎学術集会
  • Place of Presentation: 横浜
• [Presentation] アストロサイトの脳内部位特異性の解析. (2008)
  • Author(s): 青山峰芳, 垣田博樹, 加藤晋, 藤田政隆, 祖父江和哉, 浅井清文
  • Organizer: 第13回グリア研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-08
• [Presentation] アストロサイトの脳内部位特異性の解析 (2008)
  • Author(s): 青山峰芳
  • Organizer: 第13回グリア研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-08
• [Presentation] Analysis of a BAC transgenic mouse line in adulthood: distribution of genomic recombination by gfap-promotor-derived cre in the brain. (2008)
  • Author(s): Fujita, M., Ogasawara, O., Tanaka, M., Aoyama, M., Iwasato, T., Asai, K., Yamada, K., Itohara, S.
  • Organizer: Neuroscience 2008, the Society for Neuroscience's 38th annual meeting
  • Place of Presentation: Washington, DC, U.S.A.
• [Presentation] Region-specific expression of a water nervous system. (2008)
  • Author(s): Aoyama, M., Kakita, H., Kato, S., Fujita, M., Sobue, K., Asai, K.
  • Organizer: Neuroscience 2008, the Society for Neuroscience's 38th annual meeting
  • Place of Presentation: Washington, DC, U.S.A.
• [Presentation] 関節水症におけるアクアポリン発現の検討. (2008)
  • Author(s): 長原正静, 永谷祐子, 山上貴也, 青山峰芳, 浅井清文, 多田豊曠, 大塚隆信
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
• [Presentation] FK 506はリウマチ由来線維芽細胞様滑膜細胞においてgliostatin産生を抑制する. (2008)
  • Author(s): 山上貴也, 永谷祐子, 長原正静, 青山峰芳, 浅井清文, 大塚隆信
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
• [Presentation] カオリン水頭症モデルラットにおけるアクアポリン発現に関しての検討. (2008)
  • Author(s): 藤田政隆, 間瀬光人, 磯村健一, 青山峰芳, 浅井清文, 山田和雄
  • Organizer: 日本脳神経外科学会第67回学術総会
  • Place of Presentation: 盛岡
• [Presentation] アストロサイトの部位特異性に関わる分子の同定. (2008)
  • Author(s): 青山峰芳, 垣田博樹, 加藤晋, 藤田政隆, 祖父江和哉, 浅井清文
  • Organizer: 第51回日本神経化学会大会
  • Place of Presentation: 富山
• [Presentation] 乳酸アシドーシスは水チャネル<アクアポリン>のアストロサイト細胞膜への集積を促進する. (2008)
  • Author(s): 祖父江和哉, 森島徹朗, 青山峰芳, 高柳猛彦, 浅井清文
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
• [Presentation] Dexamethasone Inhibits Down Regulation of AQP3 Stimulated by TNF-α (2008)
  • Author(s): Murai, H., Aoyama, M., Terada, A., Hussein, A. H, Fujisawa, T., Asai, K., Togari, H.
  • Organizer: AAAAI(American academy of allergy asthma & immunology) annual meeting
  • Place of Presentation: Philadelphia USA
• [Presentation] Expression of aquaporin in synovial tissue of osteoarthritis and rheumatoid arthritis. (2008)
  • Author(s): 長原正静
  • Organizer: 第52回日本リウマチ学会総会・学術集会
  • Place of Presentation: 札幌
• [Presentation] Lactic acid increases aquporin 4 expression on the cell membrane of cultured rat. (2008)
  • Author(s): 祖父江和哉
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
• [Presentation] Identification of the molecules, which are responsible for astrological heterogeneity in the central nervous system. (2008)
  • Author(s): 青山峰芳
  • Organizer: 第51回日本神経化学会大会
  • Place of Presentation: 富山
• [Presentation] カオリン水頭症モデルラットにおけるアクアポリン発現に関しての検討 (2008)
  • Author(s): 藤田政隆
  • Organizer: 日本脳神経外科学会第67回学術総会
  • Place of Presentation: 盛岡
• [Presentation] 関節水症におけるアクアポリン発現の検討 (2008)
  • Author(s): 長原正静
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
• [Presentation] FK506はリウマチ由来線維芽細胞様滑膜細胞においてgliostatin産生を抑制する (2008)
  • Author(s): 山上貴也
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
• [Presentation] Novel Eras plays crucial role for epithelial to mesenchymal transition and liver metastases in human gastric cancer. (2008)
  • Author(s): 久保田英嗣
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] Analysis of a BAC transgenic mouse line in adulthood : distribution of genomic recombination by gfap-promotor-derived cre in the brain. (2008)
  • Author(s): Fujita, M.
  • Organizer: Neuroscience 2008, the Society for Neuroscience's 38th annual meeting
  • Place of Presentation: Washington, DC, U.S.A.
• [Presentation] 海馬での神経新生に関わる脳障害による顆粒下層での分子メカニズムの変化. (2007)
  • Author(s): 青山峰芳, Sherry Grissom, Julia Gohlke, 浅井清文, G. Jeen Harry
  • Organizer: 第50回日本神経化学大会
  • Place of Presentation: 横浜
• [Presentation] 乳酸アシドーシスは培養アストロサイトにおける水チャネルアクアポリン4の発現を増加させる. (2007)
  • Author(s): 森島徹朗, 祖父江和哉, 飯田裕子, 大原健太郎, 高柳猛彦, 平手博之, 青山峰芳, 有馬一, 藤田義人, 津田喬子, 勝屋弘忠、浅井清文
  • Organizer: 第50回日本神経化学大会
  • Place of Presentation: 横浜
• [Presentation] 海馬での神経新生に関わる脳障害による顆粒下層での分子メカニズムの変化 (2007)
  • Author(s): 青山峰芳
  • Organizer: 第50回日本神経化学大会
  • Place of Presentation: 横浜