Analyses of egg white specific T cell response and regulatory T cells in hen's egg allergic patients.

• Project/Area Number: 19591267
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Fujita Health University
• Principal Investigator: TSUGE Ikuya Fujita Health University, 医学部, 教授 (00231431)
• Co-Investigator(Kenkyū-buntansha): URISU Atsuo 藤田保健衛生大学, 医学部, 教授 (20193972)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 鶏卵アレルギー / 特異的免疫療法 / 特異的T細胞応答 / TH1 / TH2バランス / 抑制性サイトカイン / 食物アレルギー / サイトカイン産生プロフィール / アレルゲン特異的T細胞 / 制御性T細胞

Research Abstract

Food allergen specific T cells play a central role in both pathogenesis and tolerance induction of food allergy by regulating IgE production and allergic effecter cells such as eosinophils and mast cells. In this project, we analyzed changes of egg white specific T cell responses during specific immunotherapy for hen's egg allergy. The results showed an increase of suppressive cytokine TGF-β and decreases of Th1/Th2 cytokines as the course of immunotherapy progressed. These finding might contribute to further improvement of specific immunotherapy.

Research Products

• [Journal Article] Molecular explanation for the contradiction between systemic Th17 defect and localized bacterial infection in hyper-IgE syndrome. (2009)
  • Author(s): Minegishi Y, Saito M, Nagasawa M, Takada H, Hara T, Tsuchiya S, Agematsu K, Yamada M, Kawamura N, Ariga T, Tsuge I, Karasuyama H.
  • Journal Title: J Exp Med. 206 Pages: 1291-301
  • Peer Reviewed
• [Journal Article] Short-term efficacy of tacrolimus ointment and impact on quality of life. (2009)
  • Author(s): Kondo Y, Nakajima Y, Komatsubara R, Kato M, Hirata N, Matuyama H, Kakami M, Tsuge I, Ohya Y, Urisu A.
  • Journal Title: Pediatr Int. 51 Pages: 385-9
  • NAID: 10025347019
  • Peer Reviewed
• [Journal Article] Comparison of allergenic properties of salmon (Oncorhynchus nerka) between landlocked and anadromous species. (2009)
  • Author(s): Kondo Y, Ahn J, Komatsubara R, Terada A, Yasuda T, Tsuge I, Urisu A.
  • Journal Title: Allergol Int. 58 Pages: 295-9
  • NAID: 10025109932
  • Peer Reviewed
• [Journal Article] Comparison of allergenic properties of salmon(Oncorhynchus nerka)between landlocked and anadromous species (2009)
  • Author(s): Kondo Y, et al.
  • Journal Title: Allergol Int 58 Pages: 295-9
  • Peer Reviewed
• [Journal Article] Short-term efficacy of tacrolimus ointment and impact on quality of life (2009)
  • Author(s): Kondo Y, et al.
  • Journal Title: Pediatr Int 51 Pages: 385-9
  • Peer Reviewed
• [Journal Article] Molecular explanation for the contradiction between systemic Th17 defect and localized bacterial infection in hyper-IgE syndrome (2009)
  • Author(s): Minegishi Y, et al.
  • Journal Title: J Exp Med 206 Pages: 1291-301
  • Peer Reviewed
• [Journal Article] Utility of ovomucoid-specific IgE concentrations in predicting symptomatic egg allergy. (2008)
  • Author(s): Ando H, Moverare R, Kondo Y, Tsuge I, Tanaka A, Borres MP, Urisu A.
  • Journal Title: J Allergy Clin Immunol. 122 Pages: 583-8
  • Peer Reviewed
• [Journal Article] Up-regulated cytokine-inducible SH2-containing protein expression in allergen-stimulated T cells from hen's egg-allergic patients. (2008)
  • Author(s): Nakajima Y, Tsuge I, Kondo Y, Komatsubara R, Hirata N, Kakami M, Kato M, Kurahashi H, Urisu A, Asano Y.
  • Journal Title: Clin Exp Allergy. 38 Pages: 1499-506
  • Peer Reviewed
• [Journal Article] Utility of ovomucoid-specific IgE concentrations in predicting symptomatic egg allergy. (2008)
  • Author(s): Ando H, Moverare R, Kondo Y, Tsuge I, et al.
  • Journal Title: J Allergy Clin Immunol 122 Pages: 583-8
  • Peer Reviewed
• [Journal Article] Up-regulated cytokine-inducible SH2-containing protein expression in allergen-stimulated T cells from hen's egg-allergic patients. (2008)
  • Author(s): Nakajima Y, Tsuge I, Kon do Y, et al.
  • Journal Title: Clin Exp Allergy 38 Pages: 1499-506
  • Peer Reviewed
• [Journal Article] 鶏卵アレルギー児におけるアレルゲン特異的T細胞応答の解析Source : (0914-2649)22巻2号 (2008)
  • Author(s): 中島陽一, 柘植郁哉, 湯川牧子等
  • Journal Title: 日本小児アレルギー学会誌 22 Pages: 227-232
  • Peer Reviewed
• [Journal Article] Developmental changes of FOXP3-expressing CD4+CD25+ regulatory T cells and their impairment in patients with FOXP3 gene mutations. (2007)
  • Author(s): Fuchizawa T, Adachi Y, Ito Y, Higashiyama H, Kanegane H, Futatani T, Kobayashi I, Kamachi Y, Sakamoto T, Tsuge I, Tanaka H, Banham AH, Ochs HD, Miyawaki T.
  • Journal Title: Clin Immunol. 125 Pages: 237-46
  • Peer Reviewed
• [Journal Article] Dominant-negative mutations in the DNA-binding domain of STAT3 cause hyper-IgE syndrome. (2007)
  • Author(s): Minegishi Y, Saito M, Tsuchiya S, Tsuge I, Takada H, Hara T, Kawamura N, Ariga T, Pasic S, Stojkovic O, Metin A, Karasuyama H.
  • Journal Title: Nature 448 Pages: 1058-62
  • Peer Reviewed
• [Journal Article] Transient hypogammaglobulinemia after antiepileptic drug hypersensitivity. (2007)
  • Author(s): Okumura A, Tsuge I, Kamachi Y, Negoro T, Watanabe K.
  • Journal Title: Pediatr Neurol. 36 Pages: 342-4
  • Peer Reviewed
• [Journal Article] Allergen-specific T-cell response in patients with phenytoin hypersensitivity ; simultaneous analysis of proliferation and cytokineproduction by carboxy fluorescein succinimidyl ester (CFSE) dilution assay. (2007)
  • Author(s): Tsuge I, Okumura A, Kondo Y, Itomi S, Kakami M, Kawamura M, Nakajima Y, Komatsubara R, Urisu A.
  • Journal Title: Allergol Int. 56 Pages: 149-55
  • Peer Reviewed
• [Journal Article] Phenytoin desensitization monitored by antigen specific T cell response using carboxyfluorescein succinimidyl ester dilution assay. (2007)
  • Author(s): Okumura A, Tsuge I, Kubota T, Kurahashi H, Natsume J, Negoro T, Watanabe K.
  • Journal Title: Eur J Paediatr Neurol. 11 Pages: 385-8
  • Peer Reviewed
• [Journal Article] Allergen-specific T-cell response in patients with phenytoin hypersensitivity; simultaneous analysis of proliferation and cytokine production by carboxyfluorescein succinimidyl ester(CFSE)dilution assay. (2007)
  • Author(s): Tsuge I
  • Journal Title: Allergol Int. 56 Pages: 149-55
  • NAID: 10019480343
  • Peer Reviewed
• [Journal Article] Phenytoin desensitization monitored by antigen specific T cell response using carboxyfluorescein succinimidyl ester dilution assay. (2007)
  • Author(s): Okumura A
  • Journal Title: Pediatr Neurol. 36 Pages: 342-4
  • Peer Reviewed
• [Journal Article] Developmental changes of FOXP3-expressing CD4+CD25+regulatory Tcells and their impairment in patients with FOXP3 gene mutations. (2007)
  • Author(s): Fuchizawa T
  • Journal Title: Clin Immunol. 125 Pages: 237-46
  • Peer Reviewed
• [Presentation] 食物アレルギー 診断と治療の標準化に向けての軌道設計経口免疫療法の標準化のために解決すべき課題 (2009)
  • Author(s): 柘植郁哉
  • Organizer: 第46回、日本小児アレルギー学会
  • Place of Presentation: 福岡
  • Year and Date: 2009-12-05
• [Presentation] 食物アレルギー診断と治療の標準化に向けての軌道設計経口免疫療法の標準化のために解決すべき課題 (2009)
  • Author(s): 柘植郁哉, 他
  • Organizer: 日本小児アレルギー学会
  • Place of Presentation: 福岡
  • Year and Date: 2009-12-05
• [Presentation] 食物アレルギー研究の最近の進歩 食物アレルギーの寛容誘導療法 (2008)
  • Author(s): 柘植郁哉
  • Organizer: 第20回アレルギー学会春季臨牀大会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-12
• [Presentation] 食物アレルギー研究の最近の進歩食物アレルギーの寛容誘導療法 (2008)
  • Author(s): 柘植郁哉, 中島陽一, 小松原亮等
  • Organizer: 日本アレルギー学会春季臨床大会
  • Place of Presentation: 東京
• [Presentation] 食物アレルギーの免疫学 食物アレルギーの寛解 アウトグローと経口免疫療法 (2007)
  • Author(s): 柘植郁哉
  • Organizer: 第44回日本小児アレルギー学会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-12-08
• [Presentation] シンポジウム食物アレルギーの免疫学5.食物アレルギーの寛解;アウトグローと経口免疫療法 (2007)
  • Author(s): 柘植郁哉
  • Organizer: 第44回日本小児アレルギー学会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-12-08