Development of food antigens for oral immunotherapy to hen's egg allergy

• Project/Area Number: 19591268
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Fujita Health University
• Principal Investigator: URISU Atsuo Fujita Health University, 医学部, 教授 (20193972)
• Co-Investigator(Kenkyū-buntansha): 柘植 郁哉 藤田保健衛生大学, 医学部, 准教授 (00231431) ; 近藤 康人 藤田保健衛生大学, 医学部, 准教授 (30301641)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 小児免疫 / アレルギー / 膠原病学 / 鶏卵アレルギー / 免疫療法 / オボムコイド減量加熱全卵 / TGF-βmRNA / IFN-γ / IP-10mRNA / IL-4も、mRNA / Th1 / Th2 / IL-4 / IL-13 / Eotaxin / SOCS-3 / 経口免疫寛容 / CISH

Research Abstract

The efficiency of oral immunotherapy for food allergy has been already approved in many articles. The IT is associated in varying degrees with side effects such as urticaria, abdominal pain and anaphylaxis. We have made heated and ovomucoid-reduced whole egg, upgraded hypoallergenic food antigens for IT. The IT using this egg antigen has been proved the safety and efficiency in this study. The response of Th2 cytokine (IL4) as well as Th1 cytokine (IFN-gamma) in peripheral mononuclear cells was decreased after the stimulation by egg white antigen in vitro.

Research Products

• [Journal Article] A convenient and sensitive allergy test : IgE crosslinking induced luciferase expression in cultured mast cells (2010)
  • Author(s): Nakamura R, Uchida Y, Higuchi M, Nakamura R, Tsuge I, Urisu A, Teshima R
  • Journal Title: Allergy (in press)
  • Peer Reviewed
• [Journal Article] Enzyme-linked immunosorbent assay kit for the determination of soybean protein in processed foods : Interlaboratory evaluation (2010)
  • Author(s): Sakai S, Adachi R, Akiyama H, Teshima R, Morishita N, Matsumoto T, Urisu A
  • Journal Title: J AOAC International 93 Pages: 243-248
  • Peer Reviewed
• [Journal Article] State of the art and new horizons in the diagnosis and management of egg allergy (2010)
  • Author(s): Benhamou AH, Caubet JC, Eigenmann PA, Nowak-Wegrzyn A, Marcos CP, Reche M, Urisu A
  • Journal Title: Allergy 65 Pages: 283-289
  • Peer Reviewed
• [Journal Article] Diagnosis of Food Allergy Based on Oral Food Challenge Test. (2009)
  • Author(s): Ito K, Urisu A.
  • Journal Title: Allergol Int 58 Pages: 467-474
  • NAID: 10027165291
  • Peer Reviewed
• [Journal Article] Comparison of allergen properties in salmon (Oncorhynchus nerka) from landlocked and anadromous species (2009)
  • Author(s): Kondo Y, Ahn J, Komatsubara R, Terada A, Yasuda T, Tsuge I, Urisu A
  • Journal Title: Allergology International 58 Pages: 295-299
  • Peer Reviewed
• [Journal Article] Periodate-resistant carbohydrate epitopes recognized by IgG and IgE antibodies from some of the immunized mice and patients with allergy (2009)
  • Author(s): Hino S, Matsubara T, Urisu A, Aoki N, Sato C, Okajima T, Nadano D, Matsuda T
  • Journal Title: Biochem Biophysl Res Commun 380 Pages: 632-7
  • Peer Reviewed
• [Journal Article] Oral Allergy Syndrome (2009)
  • Author(s): Kondo Y, Urisu A
  • Journal Title: Allergol Int 58 Pages: 485-491
  • NAID: 10027165361
  • Peer Reviewed
• [Journal Article] State of the art and new horizons in the diagnosis and manage ment of egg allergy (2009)
  • Author(s): Benhamou AH
  • Journal Title: Allergy 65(in press) Pages: 283-289
  • Peer Reviewed
• [Journal Article] Oral allergy syndrome (2009)
  • Author(s): Kondo Y
  • Journal Title: Allergol Int 58 Pages: 485-491
  • NAID: 10027165361
  • Peer Reviewed
• [Journal Article] Comparison of allergenic properties of salmon(Oncorhynchus nerka)between landlocked and anadromous species (2009)
  • Author(s): Kondo Y
  • Journal Title: Allergol Int 58 Pages: 295-299
  • NAID: 10025109932
  • Peer Reviewed
• [Journal Article] Specific detection of buckwheat residues in processed foods by polymerase chain reaction (2008)
  • Author(s): Yamakawa H, Akiyama H, Endo Y, Miyatake K, Sakai S, Kondo K, Toyoda M, Urisu A, Teshima R
  • Journal Title: Biosci Biotechnol Biochem 72 Pages: 2228-2231
  • NAID: 10027531449
  • Peer Reviewed
• [Journal Article] Reliable enzyme-linked immunosorbent assay for the determination of soybean proteins in processed foods (2008)
  • Author(s): Morishita N, Kamiya K, Matsumoto T, Sakai S, Teshima R, Urisu A, Moriyama T, Ogawa T, Akiyama H, Morimatshu F
  • Journal Title: J. Agric. Food Chem 56 Pages: 6818-6824
  • Peer Reviewed
• [Journal Article] Reliable enzyme-linked immunosorbent assay for the determination of walnut proteins in processed foods (2008)
  • Author(s): Doi H, Touhata Y, Shibata H, Sakai S, Urisu A, Akiyma H, Teshima R
  • Journal Title: J Agri Food Chem 56 Pages: 7625-7630
  • Peer Reviewed
• [Journal Article] Utility of ovomucoid-specific IgE concentrations in predicting symptomatic egg allergy (2008)
  • Author(s): Ando H, Moverare R, Kondo Y, Tsuge I, Tanaka A, Borres M, Urisu A
  • Journal Title: J Allergy Clin Immunol 122 Pages: 583-588
  • Peer Reviewed
• [Journal Article] Up-regulated cytokine inducible SH2-containing protein expression in allergen-stimulated T cells from hen's egg-allergic patients. (2008)
  • Author(s): Nakajima Y, Tsuge I, Kondo Y, Komatsubara R, Hirata N, Kakami M, Kato M, Kurahashi H, Urisu A, Asano Y
  • Journal Title: Clin Exp Allergy 38 Pages: 1499-1506
  • Peer Reviewed
• [Journal Article] Interlaboratory evaluation of two enzyme-linked immunesorbent assay kits for the determination of crustacean protein in processed foods (2008)
  • Author(s): Sakai S, Matsuda, R, Adachi R, Akiyama H, Maitani T, Ohno, Y., Oka, M., Abe, A., Seiki, K., Oda, H., Shiomi, K., Urisu, A.
  • Journal Title: J AOAC Int 91 Pages: 123-129
  • Peer Reviewed
• [Journal Article] Interlaboratory Evaluation of two enzyme-linked immunesorbent assay kits for the determination of crustacean protein in processed foods (2008)
  • Author(s): Sakai S
  • Journal Title: J AOAC Int 91 Pages: 123-129
  • Peer Reviewed
• [Journal Article] Up-regulated cytokine inducible SH2-containing protein expression in allergen-stimulated T cells from hen's egg-allergic patients (2008)
  • Author(s): Nakajima Y
  • Journal Title: Clin Exp Allergy 38 Pages: 1499-1506
  • Peer Reviewed
• [Journal Article] Utility of ovomucoid-specific IgE concentrations in predicting symptom atic egg allergy (2008)
  • Author(s): Ando H
  • Journal Title: J Allergy Clin Immunol 122 Pages: 583-588
  • Peer Reviewed
• [Journal Article] Allergen-specific T-cell response in patients with phenytoin hypersensitivity; simultaneous analysis of proliferation and cytokine production by carboxyfluores (2007)
  • Author(s): Tsuge I, Okumura A, Kondo Y, Itomi S, Kakami M, Kawamura M, Nakajima Y, Komatsubara R, Urisu A.
  • Journal Title: Allergol Int 56 Pages: 149-155
  • Peer Reviewed
• [Journal Article] Guidelines for Diagnosis and Management of Pediatric Food Allergy in Japan (2007)
  • Author(s): Mukoyama T
  • Journal Title: Allergol Int 56 Pages: 349-361
  • Peer Reviewed
• [Journal Article] Allergen-specific T-cell response in patients with phenytoin hypersensitivity; simultaneous an alysis of proliferation and cytokine production by carboxyfluorescein succinimidyl ester(CFSE)dilution assay (2007)
  • Author(s): Tsuge I
  • Journal Title: Allergol Int 56 Pages: 149-155
  • Peer Reviewed
• [Journal Article] Reliable and Sensitive Immunoassay for the Determination of Crustacean Protein in Processed Foods (2007)
  • Author(s): Seiki K
  • Journal Title: J Agric Food Chem 55 Pages: 9345-9350
  • Peer Reviewed
• [Presentation] 低アレルゲン化鶏卵を用いた経口免疫療法の検討 (2010)
  • Author(s): 柘植郁哉、近藤康人、安在根、湯川牧子、成瀬徳彦、小松原亮、平田典子、鈴木聖子、安藤仁志、山田一恵、小林幸子、木村守、宇理須厚雄
  • Organizer: 第22回, 日本アレルギー学会春季臨床大会
  • Place of Presentation: 京都
• [Presentation] Long-term oral immunotherapy by hypoallergenic heated and ovomucoid-reduced egg white in subjects with hen's egg allergy. (2009)
  • Author(s): Urisu A, Naruse N, Ahn J, Komatsubara R, Hirata N, Yukawa M, Suzuki S, Ando H, Kondo Y, Tsuge I, Yamada K, Kobayashi S, Kimura
  • Organizer: European Academy of Allergy and Clinical Immunology Pediatric Allergy & Asthma Meeting
  • Place of Presentation: Venice Italy
• [Presentation] 小児アレルギー研究の方向性を探る 食物アレルギー 経口免疫療法の今後 (2009)
  • Author(s): 宇理須厚雄
  • Organizer: 第46回, 日本小児アレルギー学会
  • Place of Presentation: 福岡
• [Presentation] 食物アレルギー経口負荷試験の標準化 (2009)
  • Author(s): 宇理須厚雄
  • Organizer: 第21回日本アレルギー学会春季臨床大会
  • Place of Presentation: 岐阜
• [Presentation] Long-term oral immunotherapy by hypoallergenic heated and ovomucoid-reduced egg white in subjects with hen's egg allergy (2009)
  • Author(s): Urisu A
  • Organizer: European Academy of Allergy and Clinical Immunology Pediatric Allergy & Asthma Meeting
  • Place of Presentation: Venice Italy
• [Presentation] Oral immunotherapy by heated and ovomucoid-reduced egg white to children with hen's egg hypersensitivity. (2008)
  • Author(s): Urisu A, Komatsubara R, Hirata N, Kakami M, Kawada Yasusuke, Nakajima Y, Yukawa M, Kondo Y, Tsuge I, Tokuda R, Yamada K, Kimura M
  • Organizer: Congress of European Academy of Allergology and Clinical Immunology (EAACI)
• [Presentation] Allergen analysis of canned tuna using patient's sera. (2008)
  • Author(s): Kondo Y, Nakajima Y, Komatsubara R, Tsuge I, Yasuda T, Urisu A
  • Organizer: Congress of European Academy of Allergology and Clinical Immunology (EAACI)
  • Place of Presentation: Barcelona, Spain
• [Presentation] Food Labeling and the Epidemiology of Food Allergy, Food Labeling in Japan and Food Allergen Purification (2008)
  • Author(s): Urisu A, Nakajima Y, Komatsubara R, Hirata N, Yukawa M, Kakami M, Kondo Y, Tsuge I
  • Organizer: Pediatric Academic Societies and Asian Society for Peditric Reaseach 2008 Joint Meeting
  • Place of Presentation: Honolulu, Hwaii, USA
• [Presentation] オボムコイド減量加熱全卵を含む全粥のアレルゲン性の評価 (2008)
  • Author(s): 山田一恵、平田典子、小松原亮、湯川牧子、各務美智子、鈴木聖子、安在根、近藤康人、柘植郁哉、木村守、宇理須厚雄
  • Organizer: 第58回, 日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京
• [Presentation] Oral immunotherapy by heated and ovomucoid-reduced egg white to children with hen's egg hypersensitivity (2008)
  • Author(s): Urisu A
  • Organizer: Congress of European Academy of Allergology and Clinical Immunoiogy
  • Place of Presentation: Barcelona, Spain
• [Presentation] 加熱脱オボムコイド卵白による経口免疫療法-卵白特異的Th・Th2サイトカインとIgG4の検討- (2007)
  • Author(s): 山田一惠
  • Organizer: 日本アレルギー学会秋季学術大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-11-01
• [Book] やさしい食物アレルギーの自己管理(改訂版)(編集 馬場実) (2009)
  • Author(s): 宇理須厚雄
  • Publisher: 医薬ジャーナル社
• [Book] やさしい食物アレルギーの自己管理(改訂版) (2009)
  • Author(s): 宇理須厚雄(分担)
  • Total Pages: 71
  • Publisher: 医薬ジャーナル社