Role and regulation of STAT3 in constitutive production of IL-8 in malignant melanoma cells

• Project/Area Number: 19591305
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Kobe University
• Principal Investigator: OKA Masahiro Kobe University, 医学部附属病院, 講師 (30252761)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 色素細胞学 / 悪性黒色腫 / STAT3 / アデノウイルスベクター / IL-8 / ルシフェラーゼアッセイ / Gro-α

Research Abstract

悪性黒色腫細胞において、STAT3はIL-8遺伝子のプロモーター領域の-245 to -237 bpに存在する STAT3結合sequence TTCACCAAAに結合することにより、IL-8発現をgene transcriptionレベルで促進することができる可能性を示した。悪性黒色腫細胞では恒常的にSTAT3が活性化されているため、上記メカニズムでIL-8発現が恒常的に起こっていると考えられた。

Research Products

• [Journal Article] Protein kinase C-・II represses hepatocyte growth factor-induced invasion by preventing the association of adapter protein Gab1 and phosphatidylinositol 3-kinase in melanoma cells. (2008)
  • Author(s): Oka, M., Kikkawa, U., Nishigori, C.
  • Journal Title: J. Invest. Dermatol. 128(1) Pages: 188-195
  • Peer Reviewed
• [Journal Article] Anti-proliferative activity of IL-27 on melanoma. (2008)
  • Author(s): Yoshimoto, T., Morishima, N., Mizoguchi, I., Shimizu, M., Nagai, H., Oniki, S., Oka, M. Nishigori, C., Mizuguchi, J
  • Journal Title: J. Immunol 180(10) Pages: 6527-6535
  • Peer Reviewed
• [Journal Article] Protein kinase C-βII represses hepatocyte growth factor-induced invasion by preventing the association of adapter protein Gab1 and phosphatidylinositol 3-kinase in melanoma cells. (2008)
  • Author(s): Oka, M., Kikkawa, U., Nishigori, C.
  • Journal Title: J. Invest. Dermatol. 128 Pages: 188-195
  • Peer Reviewed
• [Presentation] Enhancement of ultraviolet B-induced skin tumor development in phospholipase C ・ knockout mice is associated with decreased cell death (2008)
  • Author(s): 岡 昌宏
  • Organizer: 第67回日本癌学会
• [Presentation] Enhancement of UVB-induced skin tumor development in phospholipase C ε knockout mice is associated with decreased cell death (2008)
  • Author(s): 岡 昌宏
  • Organizer: 34th Meeting of the American Society for Photobiology
• [Presentation] Phospholipase C・ plays a crucial role in ultraviolet B-induced skin inflammation (2008)
  • Author(s): Enhancement of UVB-induced skin tumor development in phospholipase C ε knockout mice is associated with decreased cell death
  • Organizer: 第5回国際研究皮膚科学会
• [Presentation] TPA inhibits melanoma growth by inactivation of STAT3 through PKC-activated tyrosine phosphatase(s) (2000)
  • Author(s): Enhancement of UVB-induced skin tumor development in phospholipase C ε knockout mice is associated with decreased cell death
  • Organizer: XXth International Pigment Cell Conference