Functional analysis of molecules identified by comprehensive geneexpressive analysis for development of diagnosis and treatment ofmelanoma

• Project/Area Number: 19591327
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Keio University
• Principal Investigator: MATSUZAKI Yuriko Keio University, 医学部, 助教 (40255435)
• Co-Investigator(Kenkyū-buntansha): KAWAKAMI Yutaka 慶應義塾大学, 医学部, 教授 (50161287)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 皮膚腫瘍学 / 悪性黒色腫 / 網羅的遺伝子発現解析 / 癌悪性形質 / RNA干渉

Research Abstract

本研究では、DNAチップやSAGE法などの網羅的遺伝子発現解析により、我々が同定したヒト悪性黒色腫で選択的或いは高発現する新規分子のうちKU-MEL-4、KU-MEL-5、KU-MEL-6、及びKU-MEL-7について、癌細胞悪性形質への関与や免疫学的意義を明らかにし臨床応用の可能性を検討した。その結果、いずれの悪性黒色腫高発現遺伝子も悪性黒色腫の形成や転移などに関わる基本的悪性形質に関与していることが明らかとなり、診断マーカーや治療に応用できる可能性が示唆された。

Research Products

• [Journal Article] Dendritic cell based personalizedimmunotherapy based on cancerantigen research (2008)
  • Author(s): Kawakami Y, and Matsuzaki Y. (他13名)
  • Journal Title: Front Biosci 1 Pages: 1952-1958
  • Peer Reviewed
• [Journal Article] Dendritic cell based personalized immunotherapy based on cancer antigenresearch. (2008)
  • Author(s): Kawakami Y, Matsuzaki Y, and, et al.
  • Journal Title: Frontier in Bioscience 13 Pages: 1952-1958
  • Peer Reviewed
• [Journal Article] Identification of a novelcancer-testis antigen CRT2 frequently expressed in various cancers using rep-resentational differential analysis (2007)
  • Author(s): Hayashi E, Matsuzaki Y, Kawakami Y. (他6名)
  • Journal Title: Clin Cancer Res 13(21) Pages: 6267-6274
  • Peer Reviewed
• [Journal Article] Dendritic cells transducedwith autoantigen FCRLA induce cytotoxiclymphocytes and vaccinate against murineB-cell lymphoma (2007)
  • Author(s): Inozume T, Matsuzaki Y, Kawakami Y. (他4名)
  • Journal Title: J Invest Dermatol 27(12) Pages: 2818-2822
  • Peer Reviewed
• [Journal Article] Development of individualized immunotherapy based on the analysis on antitumorimmune responses to the humantumor antigens identified using immunological and genetic methods (2007)
  • Author(s): Kawakami Y, Matsuzaki Y. (他25名)
  • Journal Title: Gene Therapyapy Pages: 240-248
• [Journal Article] Dendritic cells transduced with autoantigen FCRLA induce cytotoxic lymphocytes and vaccinate against murine B-cell lymphoma (2007)
  • Author(s): Inozume T, Matsuzaki Y, Kawakami Y, et. al.
  • Journal Title: Jornal of Unvestigative Dermatology 127(12) Pages: 2818-2822
  • Peer Reviewed
• [Presentation] KU-MEL-6による細胞増殖とインテグリンを介した細胞接着によるヒト悪性黒色腫転移機構 (2008)
  • Author(s): 藤田知信、松崎ゆり子、河上裕, 他
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] KU-MEL-6(MMA1)による細胞増殖とインテグリンを介した細胞接着増強によるヒト悪性黒色腫転移機構 (2008)
  • Author(s): 藤田知信, 松崎ゆり子, 河上裕, 他
  • Organizer: 第67回 日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] Dendritic cells transduced with autoantigenFCRLA induce cytotoxic lympho- cytesand vaccinate against B-cell lym- phoma (2007)
  • Author(s): 柴垣直孝、松崎ゆり子、河上裕, 他
  • Organizer: 第11回基盤的癌免疫研究会
  • Place of Presentation: 東京
  • Year and Date: 2007-07-11
• [Presentation] Dendritic cells transduced with autoantigen FCRLA induce cytotoxic lymphocytes and vaccinate against murine B-cell lymphoma (2007)
  • Author(s): 柴垣 直孝、松崎 ゆり子、河上 裕, 他
  • Organizer: 第11回基盤的癌免疫研究会総会
  • Place of Presentation: 東京・東京大学・鉄門講堂
  • Year and Date: 2007-07-11