Development of a method of prediction for tumor cell radiosensitivity using Premature Chromosome Condensation

• Project/Area Number: 19591454
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Radiation science
• Research Institution: Niigata University
• Principal Investigator: SASAI Keisuke Niigata University, 医歯学系, 教授 (20225858)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 放射線感受性 / 未成熟染色体凝集 / HVJ-envelope / HVJ envelope / 染色体障害 / HVJ Envelope

Research Abstract

センダイウイルスエンベロープ(HVJ-E)を用いて、未成熟染色体凝集(PCC)を簡便に誘導できた。放射線照射24時間後、すなわちDNA修復がほぼ完全に終了した時点で誘導したPCCから第4染色体断片数を測定し、さらに照射前の第4染色体数で補正したところ、断片数と細胞生存率の関係は、従来の報告とほぼ一致した。HVJ-Eを用いる本方法でも放射線感受性が測定できることが示された。

Research Products

• [Journal Article] Diagnostic limitations of 10 mm thickness single-slice computed tomography for patients with suspected appendicitis (2008)
  • Author(s): Kaidu M, Oyamatu M, Sato K, Saitou A, Yamamoto S, Yoshimura N, Sasai K
  • Journal Title: Radiat Med 26(2) Pages: 63-69
  • NAID: 10024137890
  • Peer Reviewed
• [Journal Article] Dynamic computed tomography findings of malignant intraductal papillary mucinous tumor compared with invasive ductal adenocarcinoma (2007)
  • Author(s): Ozaki T, Kamura T, Ajioka Y, Shirai Y, Kurosaki I, Yamamoto S, Sasai K
  • Journal Title: Radiat Med 25(9) Pages: 446-452
  • NAID: 10019969671
  • Peer Reviewed
• [Presentation] PCC-FISH 法による放射線感受性予測法の改良 (2009)
  • Author(s): 後藤直樹、高橋俊博、笹井啓資
  • Organizer: 第11回癌治療増感研究シンポジウム
  • Place of Presentation: 奈良県奈良市
  • Year and Date: 2009-02-15
• [Presentation] PCC-FISH法による放射線感受性予測法の改良 (2009)
  • Author(s): 後藤直樹, 高橋俊博, 笹井啓資
  • Organizer: 第11回癌治療増感研究シンポジウム
  • Place of Presentation: 奈良県奈良市
  • Year and Date: 2009-02-15
• [Presentation] Chemoradiotherapy using low-dose protracted infusion of 5-FU alone is as effective as 5-FU combined with cisplatin for advanced esophageal cancer (2007)
  • Author(s): SASAMOTO R, TSUCHIDA E, ABE E, FUKUDA T, KAWAGUCHI G, SASAI K
  • Organizer: 49th Annual Meeting of the ASTRO
  • Place of Presentation: Los Angeles, CA, USA