The investigation of mechanisms about apoptosis induction by transcription factor NF-κB inhibitor parthenolide.

• Project/Area Number: 19591456
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Radiation science
• Research Institution: University of Fukui
• Principal Investigator: HAYASHI Sachiko University of Fukui, 医学部, 助教 (00218570)
• Co-Investigator(Kenkyū-buntansha): MATSUMOTO Hideki 福井大学, 高エネルギー医学研究センター, 准教授 (40142377)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: アポトーシス / NF-κB / パルテノライド / A549細胞 / 放射線増感効果

Research Abstract

PTLはwtp53遺伝子を保有するヒト肺癌A549細胞において有意な抗腫瘍増感効果を示した。そのメカニズムは転写因子NF-κB活性の阻害により細胞周期のG_1 arrest、apoptosisの誘導によるがNF-κBシグナル経路にはp53-independentであった。

Research Products

• [Journal Article] Inhibition of NF-κB by combination therapy with parthenolide and hyperthermia and kinetics of apoptosis induction and cell cycle arrest in human lung adenocarcinoma cells (2010)
  • Author(s): Hayashi S, Sakurai H, Hayashi A, Tanaka Y, Hatashita M, Shioura H
  • Journal Title: Int J Mol Med 25 Pages: 81-87
  • Peer Reviewed
• [Journal Article] Inhibition of NF-κB by combination therapy with parthenolide and hyperthermia and kinetics of apoptosis induction and cell cycle arrest in human lung adenocarcinoma cells. (2010)
  • Author(s): S Hayashi, et al.
  • Journal Title: Int.J.Mol.Med. 25 Pages: 81-87
  • Peer Reviewed
• [Journal Article] A new paradigm in radioadaptive response developing from microbeam research (2009)
  • Author(s): Matsumoto H, Tomita M, Ohtsuka K, Hatashita M
  • Journal Title: J Radiat Res 50
  • NAID: 10025913536
  • Peer Reviewed
• [Journal Article] Thermosensitization by parthenolide in human lung adenocarcinoma A549 cells and p53-and hsp72-independent apoptosis induction via the nuclear factor-κB signal pathway. (2008)
  • Author(s): S Hayashi, et al.
  • Journal Title: Int. J. Mol. Med. 21 Pages: 585-592
  • Peer Reviewed
• [Journal Article] Thermosensitization by parthenolide in human lung adenocarcinoma A549 cells and p53-and hsp72-independent apoptosis induction via the nuclear factor-κB signal pathway. (2008)
  • Author(s): S Hayashi, et. al.
  • Journal Title: Int. J. Mol. Med. 21 Pages: 585-592
  • Peer Reviewed
• [Presentation] ヒト肺癌A549細胞におけるパルテノライド及び温熱併用によるNF-κBの阻害効果とアポトーシス誘導 (2009)
  • Author(s): 林幸子、櫻井宏明.
  • Organizer: 日本放射線影響学会第52回大会
  • Place of Presentation: 広島
  • Year and Date: 2009-11-13
• [Presentation] ヒト肺癌A549細胞におけるパルテノライド及び温熱併用によるNF-κBの阻害効果とアポトーシス誘導 (2009)
  • Author(s): 林幸子
  • Organizer: 日本放射線影響学会第52回大会
  • Place of Presentation: 広島市
  • Year and Date: 2009-11-13
• [Presentation] A549細胞における温熱及びパルテノライド併用によるNF-κBの阻害効果 (2009)
  • Author(s): 林幸子
  • Organizer: 日本ハイパーサーミア学会第26回大会
  • Place of Presentation: 千葉市
  • Year and Date: 2009-09-19
• [Presentation] A549細胞における温熱及びパルテノライド併用によるNF-κBの阻害効果 (2009)
  • Author(s): 林幸子、櫻井宏明.
  • Organizer: 日本ハイパーサーミア学会第26回大会
  • Place of Presentation: 千葉
  • Year and Date: 2009-09-12
• [Presentation] ヒト肺癌A549細胞におけるパルテノライド及び温熱併用によるNF-κB活性阻害効果とアポトーシス誘導 (2009)
  • Author(s): 林幸子、畑下昌範
  • Organizer: 第11回関西ハイパーサーミア研究会第20回高温度療法臨床研究会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-25
• [Presentation] ヒト肺癌A549細胞におけるパルテノライド及び温熱併用によるNF-κB活性阻害効果とアポトーシス誘導 (2009)
  • Author(s): 林幸子
  • Organizer: 第11回関西ハイパーサーミア研究会 第20回高温度療法臨床研究会 合同研究会
  • Place of Presentation: 大阪市
  • Year and Date: 2009-07-25
• [Presentation] The effects of combination therapy with hyperthermia and parthenolide on survivals of human lung cancer A549 cells. (2009)
  • Author(s): 林幸子, et al.
  • Organizer: 25th European Society of Hyperthermic Oncology
  • Place of Presentation: Verona, Italy
  • Year and Date: 2009-06-06
• [Presentation] The effects of combination therapy with hyperthermia and parthenolide on survivals of human lung cancer A549 cells (2009)
  • Author(s): Hayashi S, Hatashita M.
  • Organizer: 25th Annual Meeting of the European Society for Hyperthermic Oncology
  • Place of Presentation: Verona in Italy
  • Year and Date: 2009-06-04
• [Presentation] The thermo-enhancement effects of NF-κB inhibitor, Parthenolide, and the mechanisms of apoptosis induction. (2008)
  • Author(s): 林幸子
  • Organizer: 第51回日本放射線影響学会
  • Place of Presentation: 北九州市
  • Year and Date: 2008-11-19
• [Presentation] Parthenolideの温熱増感効果とNF-κB阻害経路によるアポトーシス誘導への影響 (2008)
  • Author(s): 林幸子
  • Organizer: 第25回日本ハイパーサーミア学会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-09-12
• [Presentation] Thermosensitization by parthenolide in human lung adenocarcinoma A549 cells and apoptosis induction via NF-κB signal pathway. (2008)
  • Author(s): S Hayashi, et al.
  • Organizer: 10th Int. Congr. Hyperthermic Oncol.
  • Place of Presentation: Munich, Germany
  • Year and Date: 2008-04-09
• [Presentation] ヒト肺癌A549細胞におけるNF-κB阻害剤Parthenolideの温熱増感効果 (2007)
  • Author(s): 林 幸子
  • Organizer: 日本放射線影響学会 第50回大会
  • Place of Presentation: 千葉市
  • Year and Date: 2007-11-15
• [Presentation] ヒト肺癌A549細胞におけるNF-κB阻害剤Parthenolideの温熱増感効果 (2007)
  • Author(s): 林 幸子
  • Organizer: 日本ハイパーサーミア学会 第24回大会
  • Place of Presentation: 前橋市
  • Year and Date: 2007-09-14
• [Presentation] The relation of p53 status to the radio- or thermo-enhancement effect by Adriamycin(ADM)in human lung adenocarcinoma A549 cells and kinetics of apoptosis and hsp72 protein. (2007)
  • Author(s): S. Hayashi
  • Organizer: 13th Int. Congr. Radiat. Res.
  • Place of Presentation: SanFrancisco, USA
  • Year and Date: 2007-07-11
• [Presentation] ヒト肺癌A549細胞におけるNF-κB阻害剤Parthenolideの温熱増感効果 (2007)
  • Author(s): 林 幸子
  • Organizer: 第9回関西ハイパーサーミア研究会、第17回高温度療法臨床研究会 合同研究会
  • Place of Presentation: 大阪市
  • Year and Date: 2007-06-23