Research of proteins involved in repair of DNA double strand breaks for individualization of radi ation therapy

• Project/Area Number: 19591462
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Radiation science
• Research Institution: Sapporo Medical University
• Principal Investigator: SAKATA Koh-ichi Sapporo Medical University, 医学部, 准教授 (10235153)
• Co-Investigator(Kenkyū-buntansha): TAKAGI Masaru 札幌医科大学, 医学部, 研究員 (10404716)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 放射線治療学 / DNA-PK活性 / 染色体不安定性 / 発癌

Research Abstract

これまでの我々のDNA-PK(DNA依存性プロテインキナーゼ)の研究成果を臨床応用に近づけることを目標として、DNA-PK活性を阻害して、放射線による殺細胞効果を増感する方法の開発を目指した。そこで、ギメラシルに注目して研究を行った。ギメラシルは、抗癌剤TS-1の一成分であり、肝臓での5-FUの分解を阻害し、血中5-FU濃度を高値に保つ作用をもつ。しかし、ギメラシル自体が放射線増感効果を持つことが報告されており、その放射線増感の分子メカニズムを検討した。

Research Products

• [Journal Article] Brachytherapy for oral tongue cancer : An analysis of treatment results with various biological markers (2008)
  • Author(s): K Sakata, M Someya, H Nagakura, K Nakata, A Oouchi, M Takagi, and M Hareyama
  • Journal Title: Jap J Clin Oncol 38 Pages: 402-407
  • Peer Reviewed
• [Journal Article] Results of concomitant chemoradiation for cervical cancer using high dose rate intracavitary brachytherapy : Study of JROSG (Japan Radiation Oncology Study Group) (2008)
  • Author(s): K. Sakata, H Sakurai, Y Suzuki, S Katoh,, T Ohno, T Toita, M Kataoka, E Tanaka, Y Kaneyasu, T Uno, Y Harima, and T Nakano
  • Journal Title: Acta Oncologica 47 Pages: 434-441
  • Peer Reviewed
• [Journal Article] Tissue oxygenation in a murine SCC VII tumor after X-ray irradiation as determined by EPR spectroscopy (2008)
  • Author(s): H. Fujii, K. Sakata, Y. Katsumata, R. Sato, M. Kinouchi, M. Someya, S. Masunaga, M. Hareyama, H. M. Swartz and H. Hirata
  • Journal Title: Radiother Oncol 86(23) Pages: 354-360
  • Peer Reviewed
• [Journal Article] Brachytherapy for oral tongue cancer : An analysis of treatment results with various biological markers (2008)
  • Author(s): Sakata K
  • Journal Title: Jpn J Clin Oncol 38(6) Pages: 402-407
  • Peer Reviewed
• [Journal Article] Ability to repair DNA double-strand breaks related to cancer susceptibility and radiosensitivity (2007)
  • Author(s): K Sakata, M Someya, Y Matsumoto, and M Hareyama
  • Journal Title: Radiation Medicine 25(9) Pages: 433-438
  • Peer Reviewed
• [Journal Article] 多分割照射の基礎と臨床-多分割照射の理論の臨床への応用 (2007)
  • Author(s): 坂田耕一、染谷正則、中田健生、高木克、小島一男、晴山雅人
  • Journal Title: 頭頚部癌 33(3) Pages: 280-282
  • NAID: 10019775793
• [Journal Article] Immunohistochemical analysis of Ku70/86 expression of breast cancer tissues (2007)
  • Author(s): M Someya, K Sakata, Y Matsumoto, M Satoh, H Narimatsu, and M Hareyama
  • Journal Title: Oncology Reports 18(6) Pages: 1483-1487
  • Peer Reviewed
• [Journal Article] Association of DNA-PK activity and radiation-induced NBS1 foci formation in lymphocytes with clinical malignancy in breast cancer patients (2007)
  • Author(s): M Someya, K Sakata, Y Matsumoto, H Tauchi, H Narimatsu, and M Hareyama
  • Journal Title: Oncology Reports 18(4) Pages: 873-878
  • Peer Reviewed
• [Journal Article] 発癌、放射線感受性とDNA2本鎖切断修復能 (2007)
  • Author(s): 坂田耕一, 染谷正則, 晴山雅人
  • Journal Title: 癌の臨床 53(6) Pages: 361-364
  • Peer Reviewed
• [Journal Article] cDNA analysis of gene expression associated with DNA-dependent protein kinase activity (2007)
  • Author(s): K. Sakata, H. Yamamoto, Y. Matsumoto, M. Someya , and M. Hareyama
  • Journal Title: Int J Oncol 30(2) Pages: 413-420
  • Peer Reviewed
• [Journal Article] cDNA analysis of gene expression associated with DNA-dependent protain kinase activity. (2007)
  • Author(s): K. Sakata
  • Journal Title: Int J Oncol 30 Pages: 413-420
• [Journal Article] 発癌、放射線感受性とDNA 2本鎖切断修復能 (2007)
  • Author(s): 坂田耕一
  • Journal Title: 癌の臨床 53 Pages: 361-364
  • Peer Reviewed
• [Journal Article] Association of DNA-PK activity and radiation-induced NBS1 foci formation in lymphocytes with clinical malignancy in breast cancer patients. (2007)
  • Author(s): M. Someya
  • Journal Title: Oncology Reports 18・4 Pages: 873-878
  • Peer Reviewed
• [Journal Article] Immunohistochemical analysis of Ku70/86 expression of breast cancer tissues. (2007)
  • Author(s): M. Someya
  • Journal Title: Oncology Reports 18・6 Pages: 1483-1487
  • Peer Reviewed
• [Journal Article] Ability to repair DNA double strand breaks related to cancer susceptibility and radiosensitivity (2007)
  • Author(s): K. Sakata
  • Journal Title: Radiation Medicine 25・9 Pages: 433-438
  • Peer Reviewed
• [Presentation] ギメラシルの放射線増感作用 (2008)
  • Author(s): 坂田耕一
  • Organizer: 第21回日本放射線腫瘍学会
  • Place of Presentation: 札幌パークホテル
  • Year and Date: 2008-10-18
• [Presentation] ギメラシルの放射線増感効果の分子メカニズム (2008)
  • Author(s): 坂田耕一
  • Organizer: 第66回日本医学放射線学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-04-06
• [Presentation] SCneoレポーター法によるギメラシル放射線増感効果の検討 (2007)
  • Author(s): 坂田耕一
  • Organizer: 第20回日本放射線腫瘍学会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2007-12-13