| Project/Area Number |
19591466
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Toho University |
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Principal Investigator |
ISHIKAWA Fumio Toho University, 医学部, 講師 (10130345)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAZAKI Shuichi 東邦大学, 医学部, 准教授 (30120314)
岡田 弥生 東邦大学, 医学部, 助教 (60256758)
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| Co-Investigator(Renkei-kenkyūsha) |
OKADA Yayoi 東邦大学, 医学部, 助教 (60256758)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 放射線 / CD80 / プロテオーム解析 / super-induction / 抗原提示 / サイクロヘキシミド / NF-kB転写因子 / A20-HL細胞 / Lynチロシンキナーゼ / シグナル伝達経路 / superinduction / エタノール / NF-kB / 細胞内シグナル伝達系 / CD80抗原 |
|---|
| Research Abstract |
We have previously demonstrated irradiation-induced up-regulation of CD80 expression in B lymphoma cells such as A20-HL cells. However, the mechanism of this up-regulation is unknown. In this present study, we investigated whether low-dose exposure of irradiation induces CD80 expression in mouse splenic B cells and bone marrow cells derived dendritic cells (BMDC). Selective CD80 up-regulation not only in splenic B cells but also in BMDCs. The up-regulation of CD80 expression was accompanied with nuclear factor-kB activation (NF-kB). The activation-pathway in splenic B cells was dependent on the generation of reactive oxygen species, while that in A20-HL cells and BMDCs were not.
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