| Project/Area Number |
19591500
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
TAKAHASI Masato Hokkaido University, 病院, 助教 (50374343)
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| Co-Investigator(Kenkyū-buntansha) |
高橋 弘昌 北海道大学, 大学院・医学研究科, 准教授 (30226881)
細田 充主 北海道大学, 大学病院, 医員 (40443931)
藤堂 省 北海道大学, 大学院・医学研究科, 教授 (60136463)
中西 一彰 北海道大学, 大学院・医学研究科, 特任助教 (80374338)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 乳癌 / ISET / 遺伝子解析 / 遊離癌細胞 / HER2 / FISH |
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| Research Abstract |
Exact identification of circulation tumor cells (CTC) brings the choice of patients who should take heavy treatment. As a result, Survival will be improved. At first, we confirmed the detection of very small number of breast cancer cells in blood by the isolation by size of epithelial tumor cells (ISET) method. The next, we compared the sensitivity of ISET method with that of quantitative real-time RT-PCR assay for the evaluation of CK-19 mRNA expression. The sensitivity of ISET was not comparable with those obtained using RT-PCR. Immunohistochemistry of ER, PgR, HER2 on the filter of ISET showed same behavior like the original cell lines. The DNA extracted from ISET-positive filter could detect the mutation of p53 using direct sequence method. HER-2 amplification could be measured by FISH analysis. Unfortunately none of 17 breast cancer samples from peripheral blood and bone marrow just before surgical operation, showed the presence of CTC cells on the filter of ISET method. Some devices for sensitivity improvement of ISET method should be required in the future.
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