Genetically engineered Bifidobacterium as a drug delivery system for systemic therapy of metastatic cancer patients

• Project/Area Number: 19591505
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Tokyo Medical University (2008); Shinshu University (2007)
• Principal Investigator: FUJIMORI Minoru Tokyo Medical University, 医学部, 教授 (00262725)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 腫瘍選択的治療法 / 嫌気性菌ベクター / Prodrug / Enzyme 療法 / Bifidobacterium breve / Cytosine Deaminase / Enzyme療法

Research Abstract

今回われわれは、固形癌は、その腫瘍内が正常組織に比べて嫌気的環境であることに着目し、新しい腫瘍選択的治療として嫌気性菌に遺伝子を導入する事を着想した。ビフィズス菌の一種Bifidobacterium breve( B. breve)に抗がん剤の前駆物質5FC を5FU に変換する酵素であるサイトシンデアミナーゼ遺伝子を導入しタンパク発現を確認した。さらに、担癌マウスへB. breveを全身投与した結果、腫瘍選択的成育性を証明した。

Research Products

• [Journal Article] 組換えビフィズス菌製剤を用いた固形がんの嫌気的環境を標的とした腫瘍選択的治療 (2008)
  • Author(s): 藤森実
  • Journal Title: Drug Deliverry System 23-5 Pages: 560-566
  • NAID: 10024310010
• [Journal Article] 組換えビフィズス菌製剤を用いた乳癌の腫瘍選択的治療 (2008)
  • Author(s): 藤森実
  • Journal Title: 日本臨床 66 Pages: 1211-1218
• [Journal Article] 組換えビフィズス菌製剤を用いた乳癌の腫瘍選択的治療 (2008)
  • Author(s): 藤森 実
  • Journal Title: 日本臨床 66 Pages: 1211-1218
• [Journal Article] 組換えビフィズス菌製剤を用いた固形がんの嫌気的環境を標的とした腫瘍選択的治療 (2008)
  • Author(s): 藤森 実
  • Journal Title: Drug Delivery System 23-5 Pages: 560-566
  • NAID: 10024310010
• [Journal Article] Exogeneus Cytosine Deaminase Gene Expression in Bifidobacterium breve I-53-8w for Tumor-Targeting Enzyme/Prodrug Therapy.、 Biosci (2007)
  • Author(s): Hidaka A,Hamaji Y,Sasaki T, Taniguchi S,Fujimori M
  • Journal Title: Biotechnol. Biochem 71 Pages: 2921-2926
  • Peer Reviewed
• [Journal Article] Exogeneus Cytosine Deaminase Gene Expression in Bifidobacterium breve I-53-8w for Tumor-Targeting Enzyme/Prodrug Therapy. (2007)
  • Author(s): Hidaka A, Hamaji Y, Sasaki T, Taniguchi S, Fujimori M
  • Journal Title: Biosci. Biotechnol. Biochem 71 Pages: 2921-2926
  • Peer Reviewed
• [Presentation] 嫌気的環境を標的とした転移性乳癌の腫瘍選択的治療 (2008)
  • Author(s): 藤森実
  • Organizer: 日本乳癌学会総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2008-09-27
• [Presentation] 嫌気的環境を標的とした転移性乳癌の腫瘍選択的治療 (2008)
  • Author(s): 藤森 実
  • Organizer: 第16回 日本乳癌学会総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2008-09-27
• [Presentation] Genetically engineered Bifidobacterium as a drug delivery system for systemic therapy of metastatic breast cancer patients Second JCA-AACR Special Joint Conference (2008)
  • Author(s): Fujimori M
  • Organizer: The Latest Advances in Breast Cancer Research From Basic Science to Therapeutics
  • Place of Presentation: The Westin Hotel Awaji Island Hyogo, Japan
  • Year and Date: 2008-07-15
• [Presentation] Genetically engineered Bifidobacterium as a drug delivery system for systemic therapy of metastatic breast cancer patients (2008)
  • Author(s): Minoru Fujimori
  • Organizer: Second JCA-AACR Special Joint Conference The Latest Advances in Breast Cancer Research From Basic Science to Therapeutics
  • Place of Presentation: The West in Hotel Awaji Island Hyogo, Japan
  • Year and Date: 2008-07-15
• [Presentation] Hypoxia を分子標的とした腫瘍選択的治療薬 : APS001 におけるTranslational research の現状 (2007)
  • Author(s): 藤森実
  • Organizer: 第15回日本乳癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-06-30
• [Presentation] Hypoxiaを分子標的とした腫瘍選択的治療薬:APS001におけるtranslational researchの現状 (2007)
  • Author(s): 藤森 実、佐々木貴之、浜地芳典、伊藤研一、浜 善久、天野 純、谷口純一郎
  • Organizer: 第15回日本乳癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-06-30
• [Presentation] Strong enhancement of recombinant cytosine deaminase activity in Bifidobacterium longum for tumor-targeting enzyme/prodrug therapy American Association for Cancer (2007)
  • Author(s): Hamaji Y、FujimoriM
  • Organizer: Research Annual Meeting 2007
  • Place of Presentation: Los Angeles convention center
  • Year and Date: 2007-04-15
• [Presentation] Strong enhancement of recombinant cytosine deaminase activity in Bifidobacterium longum for tumor-targeting enzyme/prodrug therapy (2007)
  • Author(s): Hamaji Y, Fujimori M, Sasaki T, Matsuhashi H, Matsui-Seki K, Shimatani-Shibata Y, Kano Y, Amano J, Taniguchi S
  • Organizer: American Association for Cancer Research Annual Meeting 2007
  • Place of Presentation: Los Angeles convention center
  • Year and Date: 2007-04-15