Development of new cancer-immunothereapy targeting cancer stem cells

• Project/Area Number: 19591509
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Kyushu University
• Principal Investigator: FUMIAKI Tanaka Kyushu University, 大学病院, 助教 (30332836)
• Co-Investigator(Kenkyū-buntansha): HIROSHI Inoue 九州大学, 生体防御医学研究所, 共同研究員 (90203249) ; SHINICHIROU Yoshitake 九州大学, 大学病院, 講師 (80315142)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 癌幹細胞 / 特異的認識 / 免疫治療 / SP / GO / 腫瘍増殖 / 多分化能

Research Abstract

消化器癌における癌幹細胞のマーカーを明らかにするとともに、その生物学的特徴を明らかにすることを第一目標として研究を行った。細胞周期を指標とした方法により癌幹細胞成分を抽出した。また、網羅的遺伝子発現解析により、癌幹細胞に特徴的な遺伝子候補を同定した。それらの遺伝子が癌幹細胞に対する新規免疫治療の実施に有用な分子である可能性が示唆された。

Research Products

• [Journal Article] Over-and under-expressed microRNAs in human colorectal cance (2009)
  • Author(s): Motoyama K, Inoue H, Takatsuno Y,Tanaka F, Mimori K, Uetake H, Sugihara K, Mori M.
  • Journal Title: Int J Oncol 34 Pages: 1069-75
• [Journal Article] Clinical significance of LAMB3 and COL7A1 mRNA in esophageal squamous cell carcinoma (2009)
  • Author(s): Kita Y, Mimori K, Tanaka F, Matsumoto T, Haraguchi N, Ishikawa K, Matsuzaki S, Fukuyoshi Y, Inoue H, Natsugoe S, Aikou T, Mori M.
  • Journal Title: Eur J Surg Oncol 35 Pages: 52-8
  • Peer Reviewed
• [Journal Article] Clinical significanceof ApoE expression in human gastriccancer (2008)
  • Author(s): Sakashita K, Tanaka F, Zhang X, MimoriK, Kamohara Y, Inoue H, Sawada T,Hirakawa K, Mori M.
  • Journal Title: Oncol Rep 20 Pages: 1313-9
• [Journal Article] Potential role of dendritic cell vaccination with MAGE peptides in gastrointestinal carcinomas (2008)
  • Author(s): Tanaka F, Haraguchi N, Isikawa K, InoueH, Mori M
  • Journal Title: Oncol Rep 20 Pages: 1111-1116
• [Journal Article] Clinical significance of lowexpression of Prostasin mRNA in human gastric cancer (2008)
  • Author(s): Sakashita K, Mimori K, Tanaka F, TaharaK, Inoue H, Sawada T, Ohira M, HirakawaK, Mori M
  • Journal Title: J Surg Oncol
• [Journal Article] CD133+CD44+ population efficiently enriches colon cancer initiating cells (2008)
  • Author(s): Haraguchi N, Ohkuma M, Sakashita H, Matsuzaki S, Tanaka F, Mimori K, Kamohara Y, Inoue H, Mori M.
  • Journal Title: Ann Surg Oncol 15 Pages: 2927-33
• [Journal Article] The search for cancer stem cells in hepatocellular carcinoma (2008)
  • Author(s): Kamohara Y, Haraguchi N, Mimori K,Tanaka F, Inoue H, Mori M, Kanematsu T
  • Journal Title: Surgery 144 Pages: 119-24
• [Journal Article] Mitotic centromere-associated kinesin is a novel marker for prognosis and lymph node metastasis in colorectal cancer (2008)
  • Author(s): Ishikawa K, Kamohara Y, Tanaka F,Haraguchi N, Mimori K, Inoue H, Mori M.
  • Journal Title: Br J Cancer 3-98 Pages: 1824-9
• [Journal Article] PDGF-BB is a novel prognostic factor in colorectal cancer (2008)
  • Author(s): Nakamura Y, Tanaka F, Yoshikawa Y,Mimori K, Inoue H, Yanaga K, Mori M
  • Journal Title: Ann Surg Oncol 15 Pages: 2129-36
• [Journal Article] Efficient identification of a novel cancer/testis antigen for immunotherapy using three-step microarray analysis (2008)
  • Author(s): Yokoe T, Tanaka F, Mimori K, Inoue H, Ohmachi T, Kusunoki M, Mori M.
  • Journal Title: Cancer Res 15-68 Pages: 1074-82
• [Journal Article] Clinical significance of BMP7 in human colorectal cancer (2008)
  • Author(s): Motoyama K, Tanaka F, Kosaka Y,Mimori K, Uetake H, Inoue H, Sugihara K,Mori M.
  • Journal Title: Ann Surg Oncol 15 Pages: 1530-7
• [Journal Article] Biological and Genetic Characteristics of Tumor-Initiating Cells in Colon Cancer (2008)
  • Author(s): Ieta K, Tanaka F, Haraguchi N, Kita Y, Sakashita H, Mimori K, Matsumoto T Inoue H, Kuwano H, Mori M.
  • Journal Title: Ann Surg Oncol 15 Pages: 638-48
• [Journal Article] Prognostic value of tumor-infiltratingFOXP3(+)regulatory T cells in patientswith hepatocellular carcinoma (2008)
  • Author(s): Sasaki A, Tanaka F, Mimori K, Inoue H, Kai S, Shibata K, Ohta M, Kitano S, Mori M.
  • Journal Title: Eur J Surg Oncol 34 Pages: 173-9
• [Journal Article] Efficient identification of a novel cancer/testis antigen for immunotherapy using three-step microarray analysis (2008)
  • Author(s): Yokoe T, Tanaka F, Mimori K, Inoue H, Ohmachi T, Kusunoki M, Mori M
  • Journal Title: Cancer Res 68 Pages: 1074-82
  • Peer Reviewed
• [Journal Article] PDGF-BB is a novel prognostic factor in colorectal cancer (2008)
  • Author(s): Nakamura Y, Tanaka F, Yoshikawa Y, Mimori K, Inoue H, Yanaga K, Mori M.
  • Journal Title: Ann Surg Oncol 15 Pages: 2129-36
  • Peer Reviewed
• [Journal Article] Mitotic centromere-associated kinesin is a novel marker for prognosis and lymph node metastasis in colorectal cancer (2008)
  • Author(s): Ishikawa K, Kamohara Y, Tanaka F, Haraguchi N, Mimori K, Inoue H, Mori M
  • Journal Title: Br J Cancer 98 Pages: 1824-9
  • Peer Reviewed
• [Journal Article] CD133+CD44+ population efficiently enriches colon cancer initiating cells. (2008)
  • Author(s): Haraguchi N, Ohkuma M, Sakashita H, Matsuzaki S, Tanaka F, Mimori K, Kamohara Y, Inoue H, Mori M
  • Journal Title: Ann Surg Oncol 15 Pages: 2927-33
  • Peer Reviewed
• [Journal Article] Potential role of dendritic cell vaccination with MAGE peptides in gastrointestinal carcinomas (2008)
  • Author(s): Tanaka F, Haraguchi N, Isikawa K, Inoue H, Mori M
  • Journal Title: Oncol Rep 20 Pages: 1111-1116
  • Peer Reviewed
• [Journal Article] Clinical significance of LAMB3 and COL7A1 mRNA in esophageal squamoos cell carcinoma (2008)
  • Author(s): Kita Y, Mimori K, Tanaka E, Matsumoto T, Haraguchi N, Ishikawa K, Matsuzaki S, Fukuyoshi Y, Inoue H, Natsugoe S, Aikou T, Mori M.
  • Journal Title: Eur J Surg Oncol
  • Peer Reviewed
• [Journal Article] Efficient identification of a novel cancer/testis antigen for immunotherapy using three-step microarray analysis (2008)
  • Author(s): Yokoe T, Tanaka F, Mimori K, Inoue H, Ohmachi T, Kusunoki M, Mori M.
  • Journal Title: Cancer Res 68 Pages: 1074-1082
  • Peer Reviewed
• [Journal Article] Clinical Significance of BMP7 in Human Colorectal Cancer (2008)
  • Author(s): Motoyama K, Tanaka F, Kosaka Y, Mimori K, Uetake H, Inoue H, Sugihara K, Mori M.
  • Journal Title: Ann Surg Oncol
  • Peer Reviewed
• [Journal Article] Prognostic value of tumor-infiltrating FOXP3+ regulatory T cells in patients with hepatocellular carcinoma (2008)
  • Author(s): Sasaki A, Tanaka F, Mimori K, Inoue H, Kai S, Shibata K, Ohta M, Kitano S, Mori M.
  • Journal Title: Eur J Surg Oncol 34 Pages: 173-179
  • Peer Reviewed
• [Journal Article] Activin a causes cancer cell aggressiveness in esophageal squamous cell carcinoma cells (2008)
  • Author(s): Yoshinaga K, Yamashita K, Mimon K, Tanaka F, Inoue H, Mori M.
  • Journal Title: Ann Surg Oncol 15 Pages: 96-103
  • Peer Reviewed
• [Journal Article] Clinical significance of the reduced expression of G protein gamma 7(GNG7)in oesophageal cancer (2008)
  • Author(s): Ohta M, Mimori K, Fukuyoshi Y, Kita Y, Motoyama K, Yamashita K, Ishii H, Inoue H, Mori M.
  • Journal Title: Br J Cancer 98 Pages: 410-417
  • Peer Reviewed
• [Journal Article] Biological and genetic characteristics of tumor-initiating cells in colon cancer (2007)
  • Author(s): Ieta K, Tanaka F, Haraguchi N, Kita Y, Sakashita H, Mimori K, Matsumoto T, Inoue H, Kuwano H, Mori M.
  • Journal Title: Ann Surg Oncol 15 Pages: 638-648
  • Peer Reviewed
• [Journal Article] Clinical significance of growth differentiation factor 11 in colorectal cancer (2007)
  • Author(s): Yokoe T, Ohmachi T, Inoue H, Mimori K, Tanaka F, Kusunoki M, Mori M.
  • Journal Title: Int J Oncol 31 Pages: 1097-1101
  • Peer Reviewed
• [Journal Article] Clinicopathological and biological significance of mitotic centromere-associated kinesin overexpression in human gastric cancer (2007)
  • Author(s): Nakamura Y, Tanaka F, Haraguchi N, Mimori K, Matsumoto T, Inoue H, Yanaga K, Mori M.
  • Journal Title: Br J Cancer 97 Pages: 543-549
  • Peer Reviewed
• [Journal Article] Clinical significance of the loss of MATS1 mRNA expression in colorectal cancer (2007)
  • Author(s): Kosaka Y, Mimori K, Tanaka F, Inoue H, Watanabe M, Mori M
  • Journal Title: Int J Oncol 31 Pages: 333-338
  • Peer Reviewed
• [Journal Article] Tripartite motif-containing 29(TRIM29)is a novel marker for lymph node metastasis in gastric cancer (2007)
  • Author(s): Koska Y, Inoue H, Ohmachi T, Yokoe T, Matsumoto T, Mimori K, Tanaka F, Watanabe M, Mori M.
  • Journal Title: Ann Surg Oncol 14 Pages: 2543-2549
  • Peer Reviewed
• [Journal Article] Identification of overexpressed genes in hepatocellular carcinoma,with special reference to ubiquitin-conjugating enzyme E2C gene expression (2007)
  • Author(s): Ieta K, Ojima E, Tanaka F, Nakamura Y, Haraguchi N, Mimori K, Inoue H, Kuwano H, Mori M
  • Journal Title: Int J Cancer 121 Pages: 33-38
  • Peer Reviewed
• [Journal Article] TIMP-3 and Phosphatidylinositol 3-kinase genes were found to be related to the progression of colon cancer in a comparison of pneumoperitoneum and laparotomy in a murine model (2007)
  • Author(s): Tanaka F, Sonoda H, Okamoto M, Mimori K, Utsunomiya T, Inoue H, Mori M.
  • Journal Title: Surg Today 37 Pages: 220-225
  • NAID: 10018873190
  • Peer Reviewed
• [Journal Article] BAC clones related to prognosis in patients with esophageal squamous carcinoma:an array comparative genomic hybridization study (2007)
  • Author(s): Hirasaki S, Noguchi T, Mimori K, Onuki J, Morita K, Inoue H, Sugihara K, Mori M, Hirano T
  • Journal Title: Oncologist 12 Pages: 406-417
  • Peer Reviewed
• [Journal Article] Clinicopathological significance of stanniocalcin 2(STC2)gene expression in colorectal cancer
  • Author(s): Ieta K , Tanaka F, Yokobori T, Kita Y,Haraguchi N, Mimori K, Kato H,Asao T, Inoue H, Kuwano H, Mori M
  • Journal Title: Int J Cancer (in press)
• [Presentation] 樹状細胞と癌抗原を用いた進行癌に対する癌ワクチン治療 ; 未来への工夫 (2008)
  • Author(s): 田中文明、横江 毅、石川健二、三森功士、田原光一郎、井上 裕、森 正樹
  • Organizer: 第70回日本臨床外科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-29
• [Presentation] 樹状細胞と癌抗原を用いた進行癌に対する癌ワクチン治療 ; 未来への工夫 (2008)
  • Author(s): 田中文明, 横江 毅, 石川健二, 三森功士, 田原光一郎, 井上裕, 森正樹
  • Organizer: 第70回日本臨床外科学会総会
  • Place of Presentation: 東京都
  • Year and Date: 2008-11-29
• [Presentation] 消化器癌への樹状細胞と癌抗原を用いた癌ワクチン治療 ; これまでとこれからへ (2008)
  • Author(s): 田中文明、原口直紹、横江 毅、石川健二、三森功士、蒲原行雄、井上 裕、森正樹
  • Organizer: 第63回日本消化器外科学会総会、パネルディスカッション
  • Place of Presentation: 札幌
  • Year and Date: 2008-07-17
• [Presentation] 消化器癌幹細胞を標的とした新規癌治療の開発 (2008)
  • Author(s): 田中文明、原口直紹、石川健二、三森功士、蒲原行雄、井上 裕、森 正樹
  • Organizer: 第108回日本外科学会定期学術集会
  • Place of Presentation: 長崎
  • Year and Date: 2008-05-16
• [Presentation] 消化器癌幹細胞を標的とした新規癌治療の開発 (2008)
  • Author(s): 田中文明, 原口直紹, 石川健二, 三森功士, 蒲原行雄, 井上裕, 森 正樹
  • Organizer: 第108回日本外科学会定期学術集会
  • Place of Presentation: 長崎市
  • Year and Date: 2008-05-16
• [Presentation] 食道癌の発生・進展に関わる因子の解析 (2007)
  • Author(s): 田中 文明, 三森 功士, 蒲原 行雄, 井上 裕, 森 正樹
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-05
• [Remarks]
  • URL: http://www.mib-beppu.kyushu-u.ac.jp/MIB_res/Surgery/