The analysis of the gene cluster and biological function about the gastric cancer cell lines highly metastatic to liver and lymph node metastasis

• Project/Area Number: 19591550
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Sapporo Medical University
• Principal Investigator: YAMAGUCHI Koji Sapporo Medical University, 医学部, 助教 (60315512)
• Co-Investigator(Kenkyū-buntansha): KIMURA Yasutoshi 札幌医科大学, 医学部, 講師 (80311893) ; MIZUGUCHI Toru 札幌医科大学, 医学部, 講師 (30347174) ; HIRATA Koichi 札幌医科大学, 医学部, 講師 (50136959)
• Co-Investigator(Renkei-kenkyūsha): YAMAGUCHI Koji 札幌医科大学, 医学部, 助教 (60315512)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 癌 / 同所性移植 / 肝転移 / リンパ節転移

Research Abstract

胃癌細胞株の細胞浮遊液をマウス胃壁に接種する細胞注入法を用いた同所性移植により、リンパ節転移モデル、肝転移モデルを樹立した。高リンパ節転移株における細胞表面接着分子の発現ではインテグリンの関与が、高肝転移株における転移成立には血管新生作用の関与が示唆された。AZ521より樹立した高リンパ節転移株AZL5Gではendothelin-A receptor、TGF-beta IIR alphaの発現増強が転移成立に強く関与していることが判明した。

Research Products

• [Journal Article] Clinical and immunological evaluation of anti-apoptosis protein, survivin-derived peptide vaccine in phase I clinical study for patients with advanced or recurrent breast cancer (2008)
  • Author(s): Tsuruma T, Iwayama Y, Ohmura T, Katsuramaki T, Hata F, Furuhata T, Yamaguchi K, Kimura Y, Torigoe T, Toyota N, Yagihashi A, Hirohashi Y, Asanuma H, Shimozawa K, Okazaki M, Mizushima Y, Nomura N, Sato N, Hirata K
  • Journal Title: J Transl Med 6 Pages: 24-24
  • Peer Reviewed
• [Journal Article] Proliferation of hepatocyte progenitor cells isolated from adult human livers in serum-free medium (2008)
  • Author(s): Sasaki K, Kon J, Mizuguchi T, Chen Q, Ooe H, Oshima H, Hirata K, Mitaka T
  • Journal Title: Cell Transplant 17(10-11) Pages: 1221-30
  • Peer Reviewed
• [Journal Article] Efficient transformation of small hepatocytes into insulin-expressing cells by forced expression of Pdx1 (2008)
  • Author(s): Kawasaki H, Mizuguchi T, Oshima H, Nobuoka T, Shibata T, Kaji S, Kokai Y, Katsuramaki T, Mitaka T, Hirata K
  • Journal Title: J Hepatobiliary Pancreat Surg 15(4) Pages: 403-9
  • NAID: 10021948664
  • Peer Reviewed
• [Journal Article] Laminin alpha 5 mediates ectopic adhesion of hepatocellular carcinoma through integrins and/or Lutheran/basal cell adhesion molecule (2008)
  • Author(s): Kikkawa Y, Sudo R, Kon J, Mizuguchi T, Nomizu M, Hirata K, Mitaka T
  • Journal Title: Exp Cell Res 314(14) Pages: 2579-90
  • Peer Reviewed
• [Journal Article] Phase I study of S-1, docetaxel and cisplatin combination chemotherapy in patients with unresectable metastatic gastric cancer (2007)
  • Author(s): Takayama T, Sato Y, Sagawa T, Okamoto T, Nagashima H, Takahashi Y, Ohnuma H, Kuroiwa G, Miyanishi K, Takimoto R, Matsunaga T, Kato J, Yamaguchi K, Hirata K, Niitsu Y
  • Journal Title: Br J Cancer 97(7) Pages: 851-6
  • Peer Reviewed
• [Journal Article] Frequent epigenetic inactivation of SFRP genes and constitutive activation of Wnt signaling in gastric cancer (2007)
  • Author(s): Nojima M, Suzuki H, Toyota M, Watanabe Y, Maruyama R, Sasaki S, Sasaki Y, Mita H, Nishikawa N, Yamaguchi K, Hirata K, Itoh F, Tokino T, Mori M, Imai K, Shinomura Y
  • Journal Title: Oncogene 26(32) Pages: 4699-713
  • Peer Reviewed