Effects of miRNA alterations on the chemosensitivity against anticancer agents in gastrointestinal tract tumors

• Project/Area Number: 19591558
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Iwate Medical University
• Principal Investigator: IKEDA Kenichiro Iwate Medical University, 医学部, 准教授 (20254758)
• Co-Investigator(Kenkyū-buntansha): MAESAWA Chihaya 岩手医科大学, 医学部, 教授 (10326647)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 消化器癌 / 化学療法 / REST / TUBB3 / miRNA / microRNA / がん / がん遺伝子 / がん抑制遺伝子 / 薬剤耐性 / ABCB1 / epigenetics / CDDP / 5-FU / Paclitaxel

Research Abstract

To investigate the relationship of miRNA alterations and chemosensitity, we investigated hsa-miR-451 and hsa-miR-498 expression in gastrointestinal tract tumors. Further, we examined also methylation and acetylation statues of TUBB3 promoter region. Demethylation and hyperacetylation at the promoter well correlated with overexpression of TBBU3 proteinn expression. Down-regulation of hsa-miR-451 and hsa-miR-498 induced ABCG2 overexpression. These results suggested that alterations of miRNA expression may contribute to chemosensitivity for anticancer drugs.

Research Products

• [Journal Article] Detection of human papillomavirus in a urothelial carcinoma mimicking urethral caruncle (2010)
  • Author(s): Tatemichi Y, Oikawa H, Maesawa C, Ambo J, Sato M, Koike H, Sata T, Fujioka T, Masuda T.
  • Journal Title: Int J Urol 17 Pages: 189-91
  • NAID: 10027257188
• [Journal Article] Association of the TNF-{alpha}-C-857T polymorphism with resistance to the cholesterol-lowering effect of HMG-CoA reductase inhibitors in type 2 diabetic subjects (2010)
  • Author(s): Takahashi T, Takahashi K, Yamashina M, Maesawa C, Kajiwara T, Taneichi H, Takebe N, Kaneko Y, Masuda T, Satoh J.
  • Journal Title: Diabetes Care 33 Pages: 463-6
• [Journal Article] Expression profiles of nestin in vascular smooth muscle cells in vivo and in vitro (2010)
  • Author(s): Oikawa H, Hayashi K, Maesawa C, Masuda T, Sobue K.
  • Journal Title: Exp Cell Res 316 Pages: 940-50
• [Journal Article] Minimally invasive surgical enucleation for esophageal leiomyoma: report of seven cases (2010)
  • Author(s): Obuchi T, Sasaki A, Nitta H, Koeda K, Ikeda K, Wakabayashi G.
  • Journal Title: Dis Esophagus 23
• [Journal Article] Enhanced goblet cell hyperplasia in HDC knockout mice with allergic airway inflammation (2009)
  • Author(s): Yamauchi K, Piao HM, Nakadate T, Shikanai T, Nakamura Y, Ito H, Mouri T, Kobayashi H, Maesawa C, Sawai T, Ohtsu H, Inoue H.
  • Journal Title: Allergol Int 58 Pages: 125-34
  • NAID: 10025109331
• [Journal Article] [A novel formulation of cisplatin (CDDP)] (2009)
  • Author(s): Sugitachi A, Kimura Y, Itabashi T, Noda H, Ishida K, Nishizuka S, Otsuka K, Kashiwaba M, Nitta H, Koeda K, Mizuno M, Sasaki A, Ikeda K, Takamori Y, Wakabayashi G.
  • Journal Title: Gan To Kagaku Ryoho 36 Pages: 2294-6
• [Journal Article] Spontaneous [Ca2+]i oscillations in G1/S phase-synchronized cells (2009)
  • Author(s): Russa AD, Maesawa C, Satoh Y.
  • Journal Title: J Electron Microsc (Tokyo) 58 Pages: 321-9
• [Journal Article] Local recurrence after surgical resection of pancreatic cancer effectively treated with combined chemoradiotherapy (2009)
  • Author(s): Obuchi T, Sasaki A, Shimooki O, Minakawa Y, Abe T, Nitta H, Otsuka K, Koeda K, Ikeda K, Wakabayashi G.
  • Journal Title: Gan To Kagaku Ryoho 36 Pages: 991-4
• [Journal Article] Differential microRNA expression between bone marrow side population cells and hepatocytes in adult mice (2009)
  • Author(s): Nagata Y, Maesawa C, Tada H, Takikawa Y, Yashima-Abo A, Masuda T.
  • Journal Title: Int J Mol Med 24 Pages: 35-43
• [Journal Article] Effect of atorvastatin on microRNA 221 / 222 expression in endothelial progenitor cells obtained from patients with coronary artery disease (2009)
  • Author(s): Minami Y, Satoh M, Maesawa C, Takahashi Y, Tabuchi T, Itoh T, Nakamura M.
  • Journal Title: Eur J Clin Invest 39 Pages: 359-67
• [Journal Article] DNA hypomethylation at the CpG island is involved in aberrant expression of the L1 cell adhesion molecule gene in colorectal cancer (2009)
  • Author(s): Kato K, Maesawa C, Itabashi T, Fujisawa K, Otsuka K, Kanno S, Tada H, Tatemichi Y, Kotani K, Oikawa H, Sugai T, Wakabayashi G, Masuda T.
  • Journal Title: Int J Oncol 35 Pages: 467-76
• [Journal Article] Loss of class III beta-tubulin induced by histone deacetylation is associated with chemosensitivity to paclitaxel in malignant melanoma cells (2009)
  • Author(s): Akasaka K, Maesawa C, Shibazaki M, Maeda F, Takahashi K, Akasaka T, Masuda T.
  • Journal Title: J Invest Dermatol 129 Pages: 1516-26
• [Journal Article] Minimally invasive surgical enucleation for esophageal leiomyoma : report of sevencases. (2009)
  • Author(s): Obuchi T, Sasaki A, Nitta H, Koeda K, Ikeda K, Wakabayashi G.
  • Journal Title: Dis Esophagus 23
  • Peer Reviewed
• [Journal Article] DNA hypomethylation at the CpG island is involved in aberrant expression of the L1 cell adhesion molecule gene in colorectal cancer. (2009)
  • Author(s): Kato K, Maesawa C, Itabashi T, Fujisawa K, Otsuka K, Kanno S, Tada H, Tatemichi Y, Kotani K, Oikawa H, Sugai T, Wakabayashi G, Masuda T.
  • Journal Title: Int J Oncol 35 Pages: 467-476
  • Peer Reviewed
• [Journal Article] Minimally invasive surgical enucleation for esophageal leiomyoma : report of seven cases. (2009)
  • Author(s): Obuchi T, Sasaki A, Nitta H, Koeda K, Ikeda K, Wakabayashi G.
  • Journal Title: Dis Esophagus (in press)
  • Peer Reviewed
• [Journal Article] Loss of class III beta-tubulin induced by histone deacetylation is associated with chemosensitivity to paclitaxel in malignant melanoma cells. (2009)
  • Author(s): Akasaka K, Maesawa C, Shibazaki M, Maeda F, Takahashi K, Akasaka T, Masuda T.
  • Journal Title: J Invest Dermatol 129 Pages: 1516-1526
  • Peer Reviewed
• [Journal Article] Progress in allergy signal research on mast cells: the role of histamine in goblet cell hyperplasia in allergic airway inflammation-a study using the Hdc knockout mouse (2008)
  • Author(s): Yamauchi K, Piao HM, Nakadate T, Shikanai T, Nakamura Y, Niisato M, Ito H, Mouri T, Kobayashi H, Maesawa C, Sawai T, Ohtsu H, Inoue H.
  • Journal Title: J Pharmacol Sci 106 Pages: 354-60
  • NAID: 10024318852
• [Journal Article] Chitin/cisplatin-an adhesive anticancer drug (2008)
  • Author(s): Sugitachi A, Otsuka K, Itabashi T, Chiba T, Kimura Y, Sasaki A, Ikeda K, Uesugi N, Takamori Y, Kurozumi S, Mori T, Wakabayashi G.
  • Journal Title: Gan To Kagaku Ryoho 35 Pages: 2021-3
• [Journal Article] Endoscopic thyroidectomy by the breast approach: a single institution's 9-year experience (2008)
  • Author(s): Sasaki A, Nakajima J, Ikeda K, Otsuka K, Koeda K, Wakabayashi G.
  • Journal Title: World J Surg 32 Pages: 381-5
• [Journal Article] Downregulation of miR-138 is associated with overexpression of human telomerase reverse transcriptase protein in human anaplastic thyroid carcinoma cell lines (2008)
  • Author(s): Mitomo S, Maesawa C, Ogasawara S, Iwaya T, Shibazaki M, Yashima-Abo A, Kotani K, Oikawa H, Sakurai E, Izutsu N, Kato K, Komatsu H, Ikeda K, Wakabayashi G, Masuda T.
  • Journal Title: Cancer Sci 99 Pages: 280-6
  • NAID: 10024002213
• [Journal Article] Epigenetic modification is involved in aberrant expression of class III beta-tubulin, TUBB3, in ovarian cancer cells (2008)
  • Author(s): Izutsu N, Maesawa C, Shibazaki M, Oikawa H, Shoji T, Sugiyama T, Masuda T.
  • Journal Title: Int J Oncol 32 Pages: 1227-35
• [Journal Article] Angiotensin II and epidermal growth factor receptor cross-talk mediated by a disintegrin and metalloprotease accelerates tumor cell proliferation of hepatocellular carcinoma cell lines (2008)
  • Author(s): Itabashi H, Maesawa C, Oikawa H, Kotani K, Sakurai E, Kato K, Komatsu H, Nitta H, Kawamura H, Wakabayashi G, Masuda T.
  • Journal Title: Hepatol Res 38 Pages: 601-13
  • NAID: 10024148920
• [Journal Article] Upper gastrointestinal disorders induced by non-steroidal anti-inflammatory drugs (2008)
  • Author(s): Chiba T, Sato K, Kudara N, Shinozaki H, Ikeda K, Endo M, Orii S, Suzuki K.
  • Journal Title: Inflammopharmacology 16 Pages: 16-20
• [Journal Article] Epigenetic modification is involved in aberrant expression of class III beta-tubulin, TUBB3, in ovarian cancer cells. (2008)
  • Author(s): Izutsu N, Maesawa C, Masuda T., et al
  • Journal Title: Int J Oncol 32 Pages: 1227-1235
  • Peer Reviewed
• [Journal Article] Downregulation of miR-138 is associated with over expression of human telomerase reverse transcriptase protein in human anaplastic thyroid carcinoma cell lines (2008)
  • Author(s): Mitomo S, Maesawa C, Ogasawara S, Iwaya T, Shibazaki M, Yashima-Abo A, Kotani K, Oikawa H, Sakurai E, Izutsu N, Kato K, Komatsu H, Ikeda K, Wakabayashi G, Masuda T.
  • Journal Title: Cancer Sci 106 Pages: 354-360
  • NAID: 10024002213
  • Peer Reviewed