Antitumor effects and promotion of liver regeneration by controlling angiogenetic signal transduction

• Project/Area Number: 19591575
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Chiba University
• Principal Investigator: SHIMIZU Shimizu Chiba University, 大学院・医学研究院, 講師 (80272318)
• Co-Investigator(Kenkyū-buntansha): MIYAZAKI Masaru 千葉大学, 大学院・医学研究院, 教授 (70166156) ; KIMURA Fumio 千葉大学, 大学院・医学研究院, 准教授 (70334208) ; OHTSUKA Masayuki 千葉大学, 医学部附属病院, 講師 (90334185) ; MITSUHASHI Noboru 千葉大学, フロンティアメディカル工学 研究開発センター, 准教授 (80400985) ; SUDA Kousuke 千葉大学, 医学部附属病院, 助教 (50400908)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 遺伝子 / 癌 / 再生医学 / 細胞 / 組織 / 細胞・組織

Research Abstract

(1)ChM-I cDNAを組み込んだ発現ベクターを作成後、肝細胞癌株に遺伝子導入したが,transfectantにおけるChM-Iがごく僅かであり、現在、再検討中である。(2)Wistar 系ラットを用い、総胆管結紮による黄疸肝モデルにて70%肝切除を行ったところ、閉塞性黄疸ではすでにHepatic stellate cellsの活性化を認め、肝切除後には残肝組織でのHGF産生のピークが消失、さらに抑制因子であるTGF-β1の産生が高く、肝再生の遅延がおこると考えられ、したがってこれらの因子のmanupulationすることにより肝再生促進の可能性が示唆された。

Research Products

• [Journal Article] Clinical Significance of Biliary Vascular Anatomy of the Right Liver for Hilar Cholangiocarcinoma Applied to Left Hemihepatectomy (2009)
  • Author(s): Shimizu H , Sawada H, Kimura F, Yoshidome H, Ohtsuka M, Kato A, Miyazaki M
  • Journal Title: Ann Surg 249 Pages: 435-439
• [Journal Article] Role of Kupffer Cells in Tolerance Induction After Portal Venous Administration of Alloantigens (2009)
  • Author(s): Shimizu H, Kataoka M, Kimura F, Yoshidome H, Ohtsuka M, Kato A, Miyazaki M.
  • Journal Title: Hepato- Gastroenterol (in press)
• [Journal Article] Induction of Antitumor Response by in Vivo Allogeneic Major Histocompatibility Complex Gene Transfer Using Electroporation (2009)
  • Author(s): Shimizu H, Nukui Y, Kimura F, Yoshidome H, Ohtsuka M, Kato A, Miyazaki M
  • Journal Title: J Surg Res (in press)
• [Journal Article] Clinical significance of biliary vascular anatomy of the right liver for hilar cholangiocarcinoma applied to left hemihepatectomy/ (2009)
  • Author(s): Shimizu H et al.
  • Journal Title: Ann Surg 249 Pages: 435-439
  • Peer Reviewed
• [Journal Article] Role oh Kupffer cells in tolerance induction after portal venous administration of alloantigens (2009)
  • Author(s): Shimizu H et al.
  • Journal Title: Hepato-Gastroenterol (In press)
  • Peer Reviewed
• [Journal Article] Induction of antitumor response by in vivo allogeneic major histocompatibility complex gene transfer using electroporation. (2009)
  • Author(s): Shimizu H et al.
  • Journal Title: J Surg Res (In press)
  • Peer Reviewed
• [Journal Article] Angiopoietin-2 levels in the hepatic vein as a useful predictor of tumor invasiveness and prognosis in human hepatocellular carcinoma (2008)
  • Author(s): Kuboki S, Shimizu H, Mitsuhashi N, Kusashio K, Kimura F, Yoshidome H, Ohtsuka M, Kato A, Yoshitomi H, Miyazaki M
  • Journal Title: J Gastroenterol Hepatol 23 Pages: 157-64
• [Journal Article] Surgical removal of occluded biliary expandable metallic stent for benig nstricture of bilioenteric anastomosis: Usefulness of Anterior Transhep atic Approarch (2008)
  • Author(s): Shinzu H, et. al.
  • Journal Title: Hepato-Gastroenterol (In press)
  • Peer Reviewed
• [Journal Article] Angiopoietin-2 1evels in the hepatic vein as a useful predictor of tumorinvasiveness and prognosisi in human hepatocellular carcinoma (2008)
  • Author(s): Kuboki S, Shimizu H, et. al.
  • Journal Title: J Gastroenterol Hepatol (In press)
  • Peer Reviewed
• [Journal Article] Aggressive surgical approach for Stage IV gallbladder carcinoma based on Japanese Society of Biliary Surgery Classification (2007)
  • Author(s): Shimizu H, Kimura F, Yoshidome F, Ohtsuka M, Kato A, Yoshitomi H, Nozawa S, Furukawa K, Mitsuhashi M, Takeuchi D,Suda K, Miyazaki M
  • Journal Title: J Hepatobiliary Pancreat Surg 14 Pages: 358-365
• [Journal Article] Aggressive surgical approach for Stage IV gallbladder carcinoma based on Japanese Society of Biliary Surgery Classification (2007)
  • Author(s): Shimizu H, et. al.
  • Journal Title: J Hepatobihary Pancreat Surg 14 Pages: 358-365
  • Peer Reviewed
• [Presentation] Induction of antitumor response by in vivo allogeneic major histocompatibility complex gene transfer using electroporation (2008)
  • Author(s): Shimizu H, Nukui Y, Kimura F, Yoshidome H, Ohtsuka M, Kato A, Miyazaki M
  • Organizer: 16th United European Gastroenterology Week
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2008-10-24
• [Presentation] Induction of antitumor response by in vivo allogeneic major histocompatibility complex gene transfer using electroporation. (2008)
  • Author(s): Shimizu H et al.
  • Organizer: 16th United European Gastroenterology Week
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2008-10-24
• [Presentation] 肝細胞癌還流領域の肝静脈血のAngiopoietin-2値と測定の意義 : 予後因子としての肝静脈血Angiopoietin-2の有用性について. (2007)
  • Author(s): 清水宏明、木村文夫、吉留博之、大塚將之、加藤厚、吉富秀幸、野沢聡志、古川勝規、三橋登、竹内男、須田浩介、吉岡伊作、宮崎勝
  • Organizer: 第107回日本外科学会定期学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2007-04-11
• [Presentation] 肝細胞癌還流領域の肝静脈血のAngiopoietin-2値と測定の意義:予後因子としての肝静脈血Angiopoietin-2の有用性について (2007)
  • Author(s): 清水 宏明, 他
  • Organizer: 第107回日本外科学会定期学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2007-04-11