Investigation of organ interaction in the innate immune system during inflammation

• Project/Area Number: 19591579
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: University of Yamanashi
• Principal Investigator: KONO Hiroshi University of Yamanashi, 大学院・医学工学総合研究部, 助教 (40322127)
• Co-Investigator(Kenkyū-buntansha): MATSUSA Masanori 山梨大学, 医学部・附属病院, 講師 (80242642) ; HIRAI Yu 山梨大学, 医学部・附属病院, 助教 (90436866)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: Kupffer細胞 / サイトカイン / 急性肺障害 / ケモカイン / 炎症性細胞腹膜炎 / 炎症性細胞 / 腹膜炎

Research Abstract

腹腔内感染に起因する急性肺障害発症機序におけるKupffer細胞(KC)の役割を検討。ラットにKC抑制物質塩化ガドリニウム(GD)を投与後、盲腸結紮穿刺腹膜炎(CLP)モデルを作成、死亡率と肺障害を検討。死亡率はコントロール(C)群で60%、GD群は12時間以内に全て死亡。GD群でC群と比較し急性肺障害が急性増悪。IL-10の発現は、GD群では発現が低下。炎症時に肺と肝臓の自然免疫系は、相互に関連しあっている事実が明らかとなった。

Research Products

• [Journal Article] Inhibition of the Kupffer cell increased serum HMGB1 levels and the mortality in a rat septic peritonitis model (2009)
  • Author(s): Kono H, Fujii H, Tsuchiya M, Hirai Y, Ishii K, Hosomura N.
  • Journal Title: J. Surg. Res In press
  • Peer Reviewed
• [Journal Article] Inhibition of the Kupffer cell increases serum high mobility group box 1 levels in a rat septic peritonitis model (2009)
  • Author(s): Hiroshi Kono
  • Journal Title: Journal of Surgical Research (In press)
  • Peer Reviewed
• [Journal Article] Inhibition of the Kupffer cell and neutralization of IL-10 increase the expression of chemokines in the lung in a rat peritonitis model (2008)
  • Author(s): Kono H, Fujii H, Tsuchiya M, Hirai Y, Ishii K, Hosomura N.
  • Journal Title: J. Surg. Res 150 Pages: 169-82
  • Peer Reviewed
• [Journal Article] Inhibition of the Kupffer cell and neutralization of IL-10 increase the expression of chemokines in the lung in a rat peritonitis model (2008)
  • Author(s): Hiroshi Kono
  • Journal Title: Journal of Surgical Research 150 Pages: 169-182
  • Peer Reviewed
• [Journal Article] Inhibition of the Kupffer cell and neutralization of IL-10 increase the expression of chemokines in the lung in a rat peritonitis model (2008)
  • Author(s): Hiroshi Kono
  • Journal Title: Journal of Surgical Research (In press)
  • Peer Reviewed
• [Presentation] 細菌性腹膜炎において、肝類洞免疫能の低下はHigh-mobility group protein (HMGB)1発現を増加させる (2008)
  • Author(s): 河野寛
  • Organizer: 第44回日本肝臓学会総会
  • Place of Presentation: 愛媛県県民文化会館
  • Year and Date: 2008-06-05
• [Presentation] 細菌性腹膜炎において、肝類洞免疫能の低下はHigh-mobility group protein (HMGB) 1発現を増加させる (2008)
  • Author(s): 河野寛
  • Organizer: 第20回日本肝胆膵外科学会・学術集会
  • Place of Presentation: 山形国際ホテル
  • Year and Date: 2008-05-29
• [Presentation] 細菌性腹膜炎において、肝類洞免疫能の低下はHigh-mobility group protein (HMGB) 1発現を増加させる (2008)
  • Author(s): 河野寛
  • Organizer: 第108回日本外科学会定期学術集会
  • Place of Presentation: 長崎ブリックホール
  • Year and Date: 2008-05-15
• [Presentation] Inhibition of the Kupffer cell increases serum high-mobility group protein levels and mortality in a rat septic peritonitis model ; organ interaction between the liver and the spleen (2008)
  • Author(s): Hiroshi Kono, Kenichi Ishii, Naohiro Hosomura, Maasto Ogiku, Hideki Fujii
  • Organizer: 59th Annual Meeting of the American Association for the Study of Liver Disease
  • Place of Presentation: San Francisco, CA, U.S.A
• [Presentation] 細菌性腹膜炎において, 肝類洞免疫能の低下はHigh-mobility group protein(HMGB)1発現を低下させる (2008)
  • Author(s): 河野寛, 石井健一, 細村直弘, 田中暢之, 藤井秀樹
  • Organizer: 第44 回日本肝臓学会総会
  • Place of Presentation: 愛媛
• [Presentation] 細菌性腹膜炎において、肝類洞免疫機能の低下はHigh-mobility group protein(HMGB)1発現を増加させる (2008)
  • Author(s): 河野寛, 石井健一, 細村直弘, 田中暢之, 藤井秀樹
  • Organizer: 第108 回日本外科学会定期学術集会
  • Place of Presentation: 長崎
• [Presentation] 炎症性腸疾患の発症機序におけるマクロファージの関与 : macrophage colony stimulating factor(M-CSF)役割 (2008)
  • Author(s): 河野寛, 石井健一, 藤井秀樹
  • Organizer: 第94 回日本消化器病学会総会
  • Place of Presentation: 福岡
• [Presentation] 細菌性腹膜炎において、肝類洞免疫機能の低下はHigh-mobility group protein (HMGB)1発現を増加させる (2008)
  • Author(s): 河野寛, 藤井秀樹
  • Organizer: 第44 回日本腹部救急医学会総会
  • Place of Presentation: 横浜
• [Presentation] 肝類洞機能低下は肺傷害を惹起する-肝肺臓器相関- (2007)
  • Author(s): 河野 寛
  • Organizer: 第19回日本肝胆膵外科学会総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-06-07
• [Presentation] 肝類洞免疫能の低下が急性肺傷害を惹起する-肝・肺臓器相関- (2007)
  • Author(s): 河野 寛
  • Organizer: 第43回日本肝臓学会総会
  • Place of Presentation: ホテルグランパシフィックメリディアン
  • Year and Date: 2007-05-31
• [Presentation] 肝類洞機能低下は肺障害を惹起する-肝肺臓器相関- (2007)
  • Author(s): 河野 寛
  • Organizer: 日本外科学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2007-05-13
• [Presentation] HCV-related proteins induce proinflammatory cytokine production by the human Kupffer cell via the MyD88-dependent signaling cascade (2007)
  • Author(s): Hiroshi Kono, Ishii Kenichi, Naohiro Hosomura, Nobuyuki Tanaka, Masanori Matsuda, Hideki Fujii
  • Organizer: 58th Annual Meeting of the American Association for the Study of Liver Disease
  • Place of Presentation: Boston
• [Presentation] Inhibition of the Kupffer cell increases serum HMGB1 levels and the mortality in a rat septic peritonitis model (2007)
  • Author(s): Hiroshi Kono, Ishii Kenichi, Naohiro Hosomura, Nobuyuki Tanaka, Masanori Matsuda, Hideki Fujii
  • Organizer: 58th Annual Meeting of the American Association for the Study of Liver Disease
  • Place of Presentation: Boston
• [Presentation] 肝類洞機能低下は肺障害を惹起する-肝肺臓器相関- (2007)
  • Author(s): 河野寛, 土屋雅人, 平井優, 雨宮秀武, 浅川真巳, 松田政徳, 藤井秀樹
  • Organizer: 第19 回日本肝胆膵外科学会総会
  • Place of Presentation: 横浜
• [Presentation] 肝類洞免疫能の低下が急性肺傷害を惹起する-肝・肺臓器相関- (2007)
  • Author(s): 河野寛, 平井優, 細村直弘, 石井健一, 田中暢之, 松田政徳, 藤井秀樹
  • Organizer: 第43 回日本肝臓学会総会
  • Place of Presentation: 東京
• [Presentation] 肝類洞免疫能の低下が急性肺障害を惹起する-肝・肺臓器相関- (2007)
  • Author(s): 河野寛, 土屋雅人, 平井優, 雨宮秀武, 浅川真巳, 松田政徳, 藤井秀樹
  • Organizer: 第107 回日本外科学会定期学術集会
  • Place of Presentation: 大阪
• [Presentation] HCV-related proteins directly activate the Kupffer cell isolated from human liver tissues (2007)
  • Author(s): Hiroshi Kono, Masato Tsuchiya, Naohiro Hosomura, Kenichi Ishii, Nobuyuki Tanaka, Masanori Matsuda, Hideki Fujii
  • Organizer: The 17th Conference of the Asian Pacific Association for the Study of the Liver
  • Place of Presentation: Kyoto
• [Presentation] Edaravone prevents acute ischemia-reperfusion injury of the liver by limiting free radical-mediated tissue damage (2007)
  • Author(s): Hiroshi Kono, Masanori Matsuda, Hideki Fujii
  • Organizer: 1st Biennial Congress of APHPBA
  • Place of Presentation: Fukuoka, Japan
• [Presentation] 診療と研究のトピックス肝類洞機能低下は肺障害を惹起する-肝肺臓器相関- (2007)
  • Author(s): 河野寛, 松田政徳, 藤井秀樹
  • Organizer: 第43回日本腹部救急医学会総会
  • Place of Presentation: 東京