Quantitative and functional analysis of mRNA using formalin fixedparaffin embedded samples of IPMN -Innovation for new treatmentof IPMN-

• Project/Area Number: 19591593
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyushu University
• Principal Investigator: NAGAI Eishi Kyushu University, 大学院医学研究院, 准教授 (30264021)
• Co-Investigator(Kenkyū-buntansha): TANAKA Masao 九州大学, 大学院・医学研究院, 教授 (30163570) ; SATO Norihiro 九州大学, 大学院・医学研究院, 助教 (20423527) ; TAKAHATA Shunichi 九州大学, 大学院・大学病院, 助教 (50437779) ; OHUCHIDA Kenoki 九州大学, 大学院・医学研究院, 特任助教 (20452708)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 膵臓外科学IPMN / FFPE / IPMN / qRT-PCR / パラフィン包埋サンプル / 膵癌 / Reg4 / DNA damage checkpoint / S100A2

Research Abstract

IPMNパラフィン包埋組織(FFPE)サンプルを用いて、その発生から浸潤・転移の各段階における腫瘍細胞を対象とし、独自に開発したmRNA定量解析法により候補遺伝子群の詳細な発現解析を行った。その結果腸型IPMNとその他のtypeのIPMNに特徴的な遺伝子を同定し、FFPEサンプルからのmRNA発現解析にも成功した。FFPEサンプルによるmRNAの発現が予後判定に有用であることを報告した。

Research Products

• [Journal Article] Reg4 expression is associated with carciniogenesis in the'intestinal'pathway of intraductalpapillary mucinous neoplasms (2009)
  • Author(s): Kohei Nakata, Eishi Nagai, KenokiOhuchida, Shinichi Aishima, AkifumiHayashi, Yoshihiro Miyasaka, Jun Yu,Kazuhiro Mizumoto, Masao Tanaka andMasazumi Tsuneyoshi
  • Journal Title: Modern Pathology 22 Pages: 460-468
  • Peer Reviewed
• [Journal Article] LMO2 is a novel predictive marker for a better prognosis in pancreatic cancer (2009)
  • Author(s): Kohei Nakata, Kenoki Ohuchida, EishiNagai, Akifumi Hayashi, YoshihiroMiyasaka, Tadashi Kayashima, Jun Yu,Shinichi Aishima, Yoshinao Oda, KazuhiroMizumoto, Masao Tanaka and MasazumiTsuneyoshi
  • Journal Title: Neoplasia (In press)
  • Peer Reviewed
• [Journal Article] Over-expression of S100A2 in pancreaticcancer correlates with progression andpoor prognosis (2009)
  • Author(s): Kenoki Ohuchida, Kazuhiro Mizumoto,Yoshihiro Miyasaka, Yu J, L Cui, HiroshiYamaguchi, Hiroki Toma, Shunichi Takahata,Norihiro Sato, Eishi Nagai, Koji Yamaguchi,Masazumi Tsuneyoshi, Masao Tanaka
  • Journal Title: J Pathol 213 Pages: 275-282
  • Peer Reviewed
• [Journal Article] Reg4 is associated with carcinogenesis in the 〓intestinal〓 pathway of intraductal papillary mucinous neoplasms (2009)
  • Author(s): Nakata K, et al
  • Journal Title: Modern Pathology 22 Pages: 460-468
  • Peer Reviewed
• [Journal Article] LIM only 4 is overexpressed in late stage pancreas cancer (2008)
  • Author(s): Yu J et.al.
  • Journal Title: Mol Cancer 7
• [Journal Article] 膵液中腫瘍マーカーと膵疾患 (2008)
  • Author(s): 大内田研宙ら
  • Journal Title: 胆と膵 29 Pages: 751-756
• [Journal Article] S100A6 is increasedin a stepwise manner during pancreaticcarcinogenesis : Clinical values ofexpression analysis in 98 pancreatic juicesamples (2007)
  • Author(s): Ohuchida K et.al.
  • Journal Title: Cancer Epidemiol Biomarkers Prev 16(4) Pages: 649-654
• [Journal Article] a noveloncogene, is upregulated in pancreaticcancer : Clinical implication of Twistexpression in pancreatic juice (2007)
  • Author(s): Ohuchida K et.al. Twist
  • Journal Title: Int J Cancer 120 Pages: 1634-1640
• [Journal Article] Twist,a novel oncogene,is upregulated in pancreatic cancer:Chnical implication of Twist expression in pancreatic juice (2007)
  • Author(s): Ohuchida K, et. al.
  • Journal Title: Int.J.Gancer 120 Pages: 1634-1640
• [Journal Article] The Role of the DNA Damage Checkpoint Pathway in Intraductal Papillary Mucinous Neoplasms of the Pancreas (2007)
  • Author(s): Miyasaka Y, et. al.
  • Journal Title: Clin Cancer Res 13 Pages: 4371-4377
• [Journal Article] Over-expression of S100A2 in pancreatic cancer correlates with progression and poor prognosis (2007)
  • Author(s): Ohuchida K, et. al.
  • Journal Title: J Pathology 213 Pages: 275-282
• [Journal Article] S100A6 is increased in a stepwise manner during pancreatic carcinogenesis: Clinical Value of expression analysis in 98 pancreatic juice samples (2007)
  • Author(s): Ohuchida K, et. al.
  • Journal Title: Cancer Epidemiol Biomarkers Prev 16 Pages: 649-654
• [Journal Article] Quantitative analysisof MUC1 and MUC5AC mRNA inpancreatic juice for preoperative diagnosisof pancreatic cancer (2006)
  • Author(s): Ohuchida K et.al.
  • Journal Title: Int J Cancer 118 Pages: 405-411
• [Journal Article] Quantitative Analysis of Human Telomerase Reverse Transcriptase in Pancreatic Cancer (2006)
  • Author(s): Ohuchida K et.al.
  • Journal Title: Clin Cancer Res 12(7) Pages: 2066-2069
• [Journal Article] S100P Is an Early Developmental Marker of Pancreatic Carcinogenesis (2006)
  • Author(s): Ohuchida K et.al.
  • Journal Title: Clin Cancer Res 12(18) Pages: 5411-5416
• [Journal Article] S100A11, A Putative Tumor Suppressor Gene, Is Overexpressed in Pancreatic Carcinogenesis (2006)
  • Author(s): Ohuchida K et.al.
  • Journal Title: Clin CancerRes 12(18) Pages: 5417-5422
• [Journal Article] Sonic hedgehog is an early developmental marker of intraductal papillary mucinous neoplasms (2006)
  • Author(s): Ohuchida K et.al.
  • Journal Title: clinical implications of mRNA levels in pancreatic juice, J Pathol 210 Pages: 42-48
• [Presentation] RegIV expression is involved in 'intestinal type'carcinogenesis of intraductal papillary mucinous neoplasm of the pancreas(poster W1407) (2008)
  • Author(s): Kohei Nakata, Eishi Nagai, KenokiOhuchida, Yoshihiro Miyasaka, AkifumiHayashi, Kazuhiro Mizumoto, Masao Tanakaand Masazumi Tsuneyoshi
  • Organizer: Digestive Disease Week 2008(San Diego)
• [Presentation] Overexpression of LMO2 in pancreatic cancer and its clinical implication as a therapeutic target (2008)
  • Author(s): Kohei Nakata, Eishi Nagai, KenokiOhuchida, Yoshihiro Miyasaka, AkifumiHayashi, Kazuhiro Mizumoto, MasazumiTsuneyoshi and Masao Tanaka
  • Organizer: Digestive Disease Week 2008(San Diego), poster W1391
• [Presentation] 膵癌におけるLMO2発現と癌化における機能解析 (2008)
  • Author(s): 仲田興平、永井英司、大内田研宙、宮坂義浩、水元一博、田中雅夫、恒吉正澄
  • Organizer: 第108回日本外科学会定期学術総会
  • Place of Presentation: 長崎
• [Presentation] Reg4 is involved in'intesinal type' carcinogenesis of intraductal papillary mucinous neoplasmof the pancreas (2008)
  • Author(s): 仲田興平、永井英司、大内田研宙、相島慎一、宮坂義浩、林 晃史、水元一博、田中雅夫、恒吉正澄
  • Organizer: 第67回日本癌学会定期学術総会
  • Place of Presentation: 名古屋
• [Presentation] RegIV expression is involved in 〓intestinal type〓 carcinogenesis of intraductal papillary mucinous neoplasm of the pancreas (2008)
  • Author(s): Nakata K, et al
  • Organizer: Digestive Disease Week 2008
  • Place of Presentation: San Diego
• [Presentation] 膵管内乳頭粘液腫瘍におけるDNA damage check point 経路の役割の検討 (2007)
  • Author(s): 宮坂義浩、永井英司、山口 浩、藤井 圭、井上崇弘、大内田研宙、山田知美、水元一博、恒吉正澄、田中雅夫
  • Organizer: 第107回日本外科学会定期学術総会
  • Place of Presentation: 大阪