Treatment for primary lung graft dysfunction by transfusion of endothelial progenitor cells transferred with intreleukin-10 gene.

• Project/Area Number: 19591608
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Tohoku University
• Principal Investigator: OKADA Yoshinori Tohoku University, 加齢医学研究所, 准教授 (90323104)
• Co-Investigator(Kenkyū-buntansha): KUBO Hiroshi 東北大学, 医学系研究科, 准教授 (20332504) ; SADO Tetsu 東北大学, 病院, 助教 (20396485) ; HOSHIKAWA Yasushi 東北大学, 加齢医学研究所, 助教 (90333814) ; KONDO Takashi 東北大学, 加齢医学研究所, 教授 (10195901) ; 松村 輔二 東北大学, 加齢医学研究所, 准教授 (80281997)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 肺移植 / 血管内皮前駆細胞 / インターロイキン-10 / GFPラット / 虚血・再灌流傷害 / 細胞移植 / 血管内皮前前駆細胞 / IL-10 / 組胞移植

Research Abstract

The purpose of this research project was to examine whether regeneration of pulmonary vascular endothelial cells that are derived from recipient cells can be observed in the rat lung in a rat model of lung transplantation, and whether transplantation of endothelial progenitor cells derived from bone marrow can facilitate regeneration of pulmonary endothelial cells. We performed rat lung transplantation from the SD rat donor to the GFPtg SD rat recipient and observed GFP(+), CD31(+) cell in the lung isograft, suggesting regeneration of endothelial cells derived from recipient cells occurs. We are planning recipient bone marrow cell transplantation to examine whether it can facilitate regeneration of pulmonary endothelial cells and also reduce ischemia-reperfusion injury of the lung isograft.

Research Products

• [Journal Article] Transbronchial human interleukin-10 gene transfer reduces acute inflammation associated with allograft rejection and intragraft interleukin-2 and tumor necrosis factor-α gene expression in a rat model of lung transplantation (2010)
  • Author(s): Oishi H, Okada Y, Kikuchi T, Hoshikawa Y, Sado T, Noda M, Endo C, Sakurada A, Matsumura Y, Kondo T
  • Journal Title: J Heart Lung Transplant 29 Pages: 360-367
  • Peer Reviewed
• [Journal Article] Preservation Solution for Endogenous Tisssue Stem Cells. (2009)
  • Author(s): T.Suzuki, H.Kubo, N.Fujino, A.E.Hegab, M.He, Y.Okada, M.Yamaya, T.Kondo.
  • Journal Title: Am J Respir Crit Care Med 179
  • Peer Reviewed
• [Journal Article] CD117 positive cells without mast cell lineage are localized in the lung parenchyma and alveolar wall in adult human lungs. (2009)
  • Author(s): T.Suzuki, H.Kubo, N.Fujino, A.E.Hegab, M.He, Y.Okada, M.Yamaya, T.Kondo
  • Journal Title: Am J Respir Crit Care Med 179
  • Peer Reviewed
• [Presentation] Preservation Solution for Endogenous Tisssue Stem Cells (2009)
  • Author(s): Suzuki T, Kubo H, Fujino N, Hegab AE, He M, Okada Y, Yamaya M, Kondo T.
  • Organizer: Am J Respir Crit Care Med 179 : 1997. American Thoracic Society
  • Place of Presentation: San Francisco
  • Year and Date: 2009-05-18